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A comparison of two procedures for labelling the surface of the hydatid disease organism, Echinococcus granulosus, with 125I

Published online by Cambridge University Press:  05 June 2009

D. P. Mcmanus ,
Diane J. Mclaren ,
N. W. T. Clark  and
R. M. E. Parkhouse
D. P. Mcmanus
Affiliation:
Department of Pure and Applied Biology, Imperial college, Prince Consort Road, London SW7 2BB;
Diane J. Mclaren
Affiliation:
Divisions of Immunology and Parasitology, National Institue for medical Research, Mill Hill, London NW7 1AA, England
N. W. T. Clark
Affiliation:
Divisions of Immunology and Parasitology, National Institue for medical Research, Mill Hill, London NW7 1AA, England
R. M. E. Parkhouse
Affiliation:
Divisions of Immunology and Parasitology, National Institue for medical Research, Mill Hill, London NW7 1AA, England
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Abstract

Living, intact protoscoleces of the British horse and sheep strains of Echinococcus granulosus were subjected to surface radioiodination procedures using 125I and Iodogen and 125I-Bolton Hunter reagent. Subsequent combined electron microscopy and autoradiography revealed specific surface membrane labelling with the Iodogen procedure, but significant tegumental labelling with the Bolton-Hunter reagent. The two parasite strains yielded different profiles of electrophoretically separated labelled proteins; the Iodogen method, not surprisingly, resulted in a less complex pattern of labelled polypeptides than the Bolton and Hunter reagent.

Keywords

Echinococcus granulosushydatidradiolabelling techniquessurface proteins
Type
Research Papers
Information
Journal of Helminthology , Volume 61 , Issue 1 , March 1987 , pp. 47 - 52
Copyright
Copyright © Cambridge University Press 1987

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References

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