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Comparative analyses of the complete mitochondrial genomes of Cyathostomum pateratum and Cyathostomum catinatum provide new molecular data for the evolution of Cyathostominae nematodes

  • Y.Y. Qiu (a1), M.H. Zeng (a1), P.W. Diao (a1), X.X. Wang (a1), Q. Li (a1), Y. Li (a1), Y. Gao (a1) and C.R. Wang (a1)...


The parasite Cyathostomum pateratum, which occurs in the large intestine of equines, is a common species of the subfamily Cyathostominae. Cyathostominae nematodes are a complex nematode group for which only limited genetic information has been reported. To re-examine the phylogenetic relationships among Cyathostominae nematodes, we sequenced the complete mitochondrial (mt) genome of Cy. pateratum and compared it with the mt genome of the congeneric species Cyathostomum catinatum. The complete mtDNA sequence of Cy. pateratum was 13,822 bp in length, 16 bp shorter than that of Cy. catinatum. The mtDNA sequences of both species contained 12 protein-coding genes, two rRNA genes and 22 tRNA genes, and all 36 genes were transcribed in the same direction and in the same strand. Pairwise comparisons of the 12 predicted amino acid sequences between Cy. catinatum and Cy. pateratum revealed differences of 0.4–3.1%; the least conserved sequence was that of cytochrome c oxidase subunit 3 (cox3). Phylogenetic analysis of the concatenated amino acid sequences using Bayesian inference and maximum likelihood methods showed that Cy. catinatum and Cy. pateratum clustered together with very high nodal support, and Cylicostephanus goldi was closer to the Cyathostomum nematodes than to other Cyathostominae nematodes. The mtDNA sequence of Cy. pateratum is reported here for the first time. The study will shed some light on the genetic evolution among parasitic nematodes in Cyathostomum.


Corresponding author

Author for correspondence: C.R. Wang, E-mail:


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Bu, Y et al. (2009) Strongyloid nematodes in the caeca of donkeys in Henan Province, China. Acta Parasitologica 54, 263268.
Burland, TG (2000) DNASTAR's Lasergene sequence analysis software. Methods in Molecular Biology 132, 7191.
Chang, QC et al. (2016) Sequencing and characterization of the complete mitochondrial genome from the pancreatic fluke Eurytrema pancreaticum (Trematoda: Dicrocoeliidae). Gene 576, 160165.
Duan, H et al. (2015) Complete mitochondrial genome of an equine intestinal parasite, Triodontophorus brevicauda (Chromadorea: Strongylidae): the first characterization within the genus. Parasitology International 64, 429434.
Gao, JF et al. (2014) Comparative analyses of the complete mitochondrial genomes of the two ruminant hookworms Bunostomum trigonocephalum and Bunostomum phlebotomum. Gene 541, 92100.
Gao, JF et al. (2017) Complete mitochondrial genomes of Triodontophorus serratus and Triodontophorus nipponicus, and their comparison with Triodontophorus brevicauda. Experimental Parasitology 181, 8893.
Gao, Y et al. (2017a) Complete mitochondrial genome of parasitic nematode Cylicocyclus nassatus, and comparative analyses with Cylicocyclus insigne. Experimental Parasitology 172, 1822.
Gao, Y et al. (2017b) Mitochondrial DNA evidence supports the hypothesis that Triodontophorus species belong to Cyathostominae. Frontiers in Microbiology 8, 1444.
Hung, GC, Chilton, NB and Gasser, RB (2000) A molecular systematic framework for equine strongyles based on ribosomal DNA sequence data. International Journal for Parasitology 30, 95103.
Jex, AR et al. (2009) The mitochondrial genomes of Ancylostoma caninum and Bunostomum phlebotomum – two hookworms of animal health and zoonotic importance. BMC Genomics 10, 79.
Lavrov, DV and Brown, WM (2001) Trichinella spiralis mtDNA: a nematode mitochondrial genome that encodes a putative ATP8 and normally structured tRNAs and has a gene arrangement relatable to those of coelomate metazoans. Genetics 157, 621637.
Lichtenfels, JR (1987) Phylogenetic inference from adult morphology in the Nematoda; with emphasis on the bursate nematodes, the strongylida; advancements (1982–1985) and recommendations for further work. International Journal for Parasitology 17, 269279.
Lichtenfels, JR, Kharchenko, VA and Dvojnos, GM (2008) Illustrated identification keys to strongylid parasites (Strongylidae: Nematoda) of horses, zebras and asses (Equidae). Veterinary Parasitology 156, 4161.
Liu, GH et al. (2012a) Comparative analyses of the complete mitochondrial genomes of Ascaris lumbricoides and Ascaris suum from humans and pigs. Gene 492, 110116.
Liu, GH et al. (2012b) Clear genetic distinctiveness between human- and pig-derived trichuris based on analyses of mitochondrial datasets. PLoS Neglected Tropical Diseases 6, e1539.
McDonnell, A et al. (2000) Phylogenetic analysis of partial mitochondrial cytochrome oxidase c subunit I and large ribosomal RNA sequences and nuclear internal transcribed spacer I sequences from species of Cyathostominae and Strongylinae (Nematoda, Order Strongylida), parasites of the horse. Parasitology 121, 649659.
Mfitilodze, M and Hutchinson, G (1990) Prevalence and abundance of equine strongyles (Nematoda: Strongyloidea) in tropical Australia. Journal of Parasitology 76, 487–449.
Smets, K et al. (1999) Diagnosis of larval cyathostominosis in Belgium. Veterinary Record 144, 665668.
Stancampiano, L et al. (2017) Are small strongyles (Cyathostominae) involved in horse colic occurrence? Veterinary Parasitology 247, 3336.
Tamura, K et al. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 27312739.
Thompson, JD et al. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 48764882.
Wang, CR et al. (2016) Comparative analyses of the complete mitochondrial genomes of the two murine pinworms Aspiculuris tetraptera and Syphacia obvelata. Gene 585, 7175.
Xu, WW et al. (2015) The complete mitochondrial genome of Strongylus equinus (Chromadorea: Strongylidae): comparison with other closely related species and phylogenetic analyses. Experimental Parasitology 159, 9499.
Zhang, Y et al. (2015) The complete mitochondrial genome of Oxyuris equi: comparison with other closely related species and phylogenetic implications. Experimental Parasitology 159, 215221.


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