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Characterization of the complete mitochondrial genome of Setaria digitata (Nematoda: Setariidae) from China

  • G.-H. Liu (a1) (a2), J.-Y. Li (a2) and X.-Q. Zhu (a1) (a2)

Abstract

Setaria digitata is a filarial parasite that causes fatal cerebrospinal nematodiasis in goats, horses and sheep, resulting in substantial economic losses to livestock farmers. In the present study, the complete mitochondrial (mt) genome of S. digitata from China was determined, characterized and compared with that of S. digitata from Sri Lanka. The identity of the mt genomes was 98.3% between S. digitata from China and Sri Lanka, and the complete mt genome sequence of S. digitata from China was slightly shorter (25 bp) than that from Sri Lanka. For the 12 protein genes, this comparison revealed sequence differences at both the nucleotide (1.4%) and amino acid (2.2%) levels. The present study determined the complete mt genome sequence of S. digitata from China, providing novel genetic markers for the study of the population genetics and molecular epidemiology of S. digitata in animals.

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Corresponding author

*Fax: +86 931 8340977 E-mail: xingquanzhu1@hotmail.com

References

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Alasaad, S., Pascucci, I., Jowers, M.J., Soriguer, R.C., Zhu, X.Q., & Rossi, L. (2012) Phylogenetic study of Setaria cervi based on mitochondrial cox1 gene sequences. Parasitology Research 110, 281285.
Chamuah, J.K., Sakhrie, A., Lama, S., Chandra, S., Chigure, G.M., Bauri, R.K. & Jacob, S.S. (2015) Molecular characterization of Setaria digitata from Mithun (Bos frontalis). Acta Parasitologica 60, 391394.
Gao, J.F., Zhao, Q., Liu, G.H., Zhang, Y., Zhang, Y., Wang, W.T., Chang, Q.C., Wang, C.R. & Zhu, X.Q. (2014) Comparative analyses of the complete mitochondrial genomes of the two ruminant hookworms Bunostomum trigonocephalum and Bunostomum phlebotomum . Gene 541, 92100.
Gasser, R.B. (2006) Molecular tools – advances, opportunities and prospects. Veterinary Parasitology 136, 6989.
Hu, M., Gasser, R.B., Abs El-Osta, Y.G. & Chilton, N.B. (2003) Structure and organization of the mitochondrial genome of the canine heartworm, Dirofilaria immitis . Parasitology 127, 3751.
Jabbar, A., Littlewood, D.T., Mohandas, N., Briscoe, A.G., Foster, P.G., Müller, F., von Samson-Himmelstjerna, G., Jex, A.R. & Gasser, R.B. (2014) The mitochondrial genome of Parascaris univalens – implications for a ‘forgotten’ parasite. Parasites & Vectors 7, 428.
Keddie, E.M., Higazi, T. & Unnasch, T.R. (1998) The mitochondrial genome of Onchocerca volvulus: sequence, structure and phylogenetic analysis. Molecular and Biochemical Parasitology 95, 111127.
Khedri, J., Radfar, M.H., Borji, H. & Azizzadeh, M. (2014) An epidemiological survey of Setaria in the abdominal cavities of Iranian sistani and brahman cattle in the southeast of Iran. Iranian Journal of Parasitology 9, 249253.
Liu, G.H., Gasser, R.B., Su, A., Nejsum, P., Peng, L., Lin, R.Q., Li, M.W., Xu, M.J. & Zhu, X.Q. (2012) Clear genetic distinctiveness between human- and pig-derived Trichuris based on analyses of mitochondrial datasets. PLoS Neglected Tropical Diseases 6, e1539.
Liu, G.H., Gasser, R.B., Otranto, D., Xu, M.J., Shen, J.L., Mohandas, N., Zhou, D.H. & Zhu, X.Q. (2013) Mitochondrial genome of the eyeworm, Thelazia callipaeda (Nematoda: Spirurida), as the first representative from the family Thelaziidae. PLoS Neglected Tropical Diseases 7, e2029.
Liu, G.H., Zhao, L., Song, H.Q., Zhao, G.H., Cai, J.Z., Zhao, Q. & Zhu, X.Q. (2014) Chabertia erschowi (Nematoda) is a distinct species based on nuclear ribosomal DNA sequences and mitochondrial DNA sequences. Parasites &Vectors 7, 44.
Liu, G.H., Jia, Y.Q., Wang, Y.N., Zhao, G.H. & Zhu, X.Q. (2015) The complete mitochondrial genome of the gullet worm Gongylonema pulchrum: gene content, arrangement, composition and phylogenetic implications. Parasites &Vectors 8, 100.
Lowe, T.M. & Eddy, S.R. (1997) tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Research 25, 955964.
McNulty, S.N., Mullin, A.S., Vaughan, J.A., Tkach, V.V., Weil, G.J. & Fischer, P.U. (2012) Comparing the mitochondrial genomes of Wolbachia-dependent and independent filarial nematode species. BMC Genomics 13, 145.
Perumal, A.N., Gunawardene, Y.I. & Dassanayake, R.S. (2016) Setaria digitata in advancing our knowledge of human lymphatic filariasis. Journal of Helminthology 90, 129138.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 48764882.
Voronin, D., Abeykoon, A.M., Gunawardene, Y.I. & Dassanayake, R.S. (2015) Absence of Wolbachia endobacteria in Sri Lankan isolates of the nematode parasite of animals Setaria digitata . Veterinary Parasitology 207, 350354.
Wang, B.J., Gu, X.B., Yang, G.Y., Wang, T., Lai, W.M., Zhong, Z.J. & Liu, G.H. (2016) Mitochondrial genomes of Heterakis gallinae and Heterakis beramporia support that they belong to the infraorder Ascaridomorpha. Infection Genetics and Evolution 40, 228235.
Xu, W.W., Qiu, J.H., Liu, G.H., Zhang, Y., Liu, Z.X., Duan, H., Yue, D.M., Chang, Q.C., Wang, C.R. & Zhao, X.C. (2015) The complete mitochondrial genome of Strongylus equinus (Chromadorea: Strongylidae): comparison with other closely related species and phylogenetic analyses. Expermental Parasitology 159, 9499.
Yatawara, L., Wickramasinghe, S., Nagataki, M., Rajapakse, R.P. & Agatsuma, T. (2007) Molecular characterization and phylogenetic analysis of Setaria digitata of Sri Lanka based on CO1 and 12S rDNA genes. Veterinary Parasitology 148, 161165.
Yatawara, L., Wickramasinghe, S., Rajapakse, R.P. & Agatsuma, T. (2010) The complete mitochondrial genome of Setaria digitata (Nematoda: Filarioidea): mitochondrial gene content, arrangement and composition compared with other nematodes. Molecular and Biochemical Parasitology 173, 3238.
Yong, H.S., Song, S.L., Eamsobhana, P., Goh, S.Y. & Lim, P.E. (2015) Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae). Acta Tropica 152, 157164.
Zhao, G.H., Wang, H.B., Jia, Y.Q., Zhao, W., Hu, X.F., Yu, S.K. & Liu, G.H. (2016) The complete mitochondrial genome of Pseudanoplocephala crawfordi and a comparison with closely related cestode species. Journal of Helminthology 90, 588595.

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