Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-24T11:37:20.005Z Has data issue: false hasContentIssue false
  • English
  • Français

Characteristics of DNase activities in excretory/secretory products of infective larvae of Haemonchus contortus

Published online by Cambridge University Press:  30 August 2011

S.H. Yun ,
M.G. Seo ,
B.Y. Jung ,
T.H. Kim ,
O.D. Kwon ,
K.S. Jeong ,
M.H. Rhee ,
Y.J. Lee ,
S.J. Park  and
Y.S. Kwon
...Show all authors
S.H. Yun
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
M.G. Seo
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
B.Y. Jung
Affiliation:
Animal Disease Diagnostic Centre, National Veterinary Research and Quarantine Service, 335 Joongangro, Anyang, Kyunggi430-824, Republic of Korea
T.H. Kim
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
O.D. Kwon
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
K.S. Jeong
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
M.H. Rhee
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
Y.J. Lee
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
S.J. Park
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
Y.S. Kwon
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
D. Kwak*
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
*
*Fax: +82-53-950-5955 E-mail: dmkwak@knu.ac.kr
Get access Rights & Permissions [Opens in a new window]

Abstract

While multiple DNase activities occur in the excretory/secretory products (ESPs) of the adult Haemonchus contortus, the DNase activities in ESPs of the infective larvae (L3) have not been studied. Thus, the DNase activities in ESPs of H. contortus L3 were investigated and compared to those of adults for developmental stage-specific analysis. The DNase activities had relative molecular masses (Mrs) of 34 and 36 kDa upon zymographic analysis at pH 5.0 and 7.0 when the larvae were incubated for over 48 h. The 34 and 36 kDa DNases of L3 ESPs were also detected in adult ESPs with similar characteristics. However, the 37 and 38.5 kDa DNases of the adult ESPs were not detected in the L3 ESPs. Since the 37 and 38.5 kDa DNase activities were mainly detected in adult ESPs, these activities appear to be specific to the adult stage whereas the other ESP DNase activities appear to be expressed during multiple stages of the parasite's life cycle. While the difference in DNase activities of L3 and adults remains obscure, the role of DNase in larval development should be further clarified and the identification of stage-specific developmental markers will lead to the discovery of specific factors that stimulate larval development.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 3 , September 2012 , pp. 363 - 367
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Borgers, M., De Nollin, S., De Brabander, M. & Thienpont, D. (1975) Influence of the anthelmintic mebendazole on microtubules and intracellular organelle movement in nematode intestinal cells. American Journal of Veterinary Research 36, 1153–1166.Google ScholarPubMed
Coles, G.C., Jackson, F., Pomroy, W.E., Prichard, R.K., von Samson-Himmelstjerna, G., Silvestre, A., Taylor, M.A. & Vercruysse, J. (2006) The detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 136, 167–185.CrossRefGoogle ScholarPubMed
Dalton, J.P., Neill, S.O., Stack, C., Collins, P., Walshe, A., Sekiya, M., Doyle, S., Mulcahy, G., Hoyle, D., Khaznadji, E., Moiré, N., Brennan, G., Mousley, A., Kreshchenko, N., Maule, A.G. & Donnelly, S.M. (2003) Fasciola hepatica cathepsin 1-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines. International Journal for Parasitology 33, 1173–1181.CrossRefGoogle Scholar
Ghosh, U., Giri, K. & Bhattacharyya, N.P. (2009) Interaction of aurintricarboxylic acid (ATA) with four nucleic acid binding proteins DNase I, RNase A, reverse transcriptase and Taq polymerase. Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 74, 1145–1151.CrossRefGoogle ScholarPubMed
Jasmer, D.P. & McGuire, T.C. (1991) Protective immunity to a blood-feeding nematode (Haemonchus contortus) induced by parasite gut antigens. Infection and Immunology 59, 4412–4417.CrossRefGoogle ScholarPubMed
Jasmer, D.P., Yao, C., Rehman, A. & Johnson, S. (2000) Multiple lethal effects induced by a benzimidazole anthelmintic in the anterior intestine of the nematode Haemonchus contortus. Molecular and Biochemical Parasitology 105, 81–90.CrossRefGoogle ScholarPubMed
Karanu, F.N., Rurangirwa, F.R., McGuire, T.C. & Jasmer, D.P. (1993) Haemonchus contortus: identification of proteases with diverse characteristics in adult worm excretory-secretory products. Experimental Parasitology 77, 362–371.CrossRefGoogle ScholarPubMed
Kopp, S.R., Kotze, A.C., McCarthy, J.S., Morton, J.M. & Coleman, G.T. (2008) Strategies for the storage of Ancylostoma caninum third-stage larvae. Journal of Parasitology 94, 755–756.CrossRefGoogle ScholarPubMed
Kwak, D. & Jasmer, D.P. (2003) Non-classic characteristics define prominent DNase activities from the intestine and other tissues of Haemonchus contortus. Experimental Parasitology 104, 131–139.CrossRefGoogle ScholarPubMed
Kwak, D. & Jasmer, D.P. (2004) Intestinal DNases of 36 and 38.5 kDa from the parasitic nematode Haemonchus contortus have non-classic DNase characteristics and produce DNA fragments with 3′-hydroxyls. Experimental Parasitology 108, 142–153.CrossRefGoogle Scholar
Liu, M.F., Wu, X.P., Wang, X.L., Yu, Y.L., Wang, W.F., Chen, Q.J., Boireau, P. & Liu, M.Y. (2008) The functions of deoxyribonuclease II in immunity and development. DNA and Cell Biology 27, 223–228.CrossRefGoogle ScholarPubMed
Mak, C.H. & Ko, R.C. (1999) Characterization of endonuclease activity from excretory/secretory products of a parasitic nematode, Trichinella spiralis. European Journal of Biochemistry 260, 477–481.CrossRefGoogle ScholarPubMed
Maruyama, H., Nishimaki, A., Takuma, Y., Kurimoto, M., Suzuki, T., Sakatoku, Y., Ishikawa, M. & Ohta, N. (2006) Successive changes in tissue migration capacity of developing larvae of an intestinal nematode, Strongyloides venezuelensis. Parasitology 132, 411–418.CrossRefGoogle ScholarPubMed
Morales, M.E., Rinald, G., Gobert, G.N., Kines, K.J., Tort, J.F. & Brindley, P.J. (2008) RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade. Molecular and Biochemical Parasitology 157, 160–168.CrossRefGoogle ScholarPubMed
Shah, P.L., Scott, S.F., Knight, R.A., Marriott, C., Ranasinha, C. & Hodson, M.E. (1996) In vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosis. Thorax 51, 119–125.CrossRefGoogle ScholarPubMed
Taylor, M.A., Coop, R.L. & Wall, R.L. (2007) Veterinary parasitology. 3rd edn. pp. 158–161. Oxford, Blackwell.Google Scholar
Yatsuda, A.P., Krijgsveld, J., Cornelissen, A.W., Heck, A.J. & de Vries, E. (2003) Comprehensive analysis of the secreted proteins of the parasite Haemonchus contortus reveals extensive sequence variation and differential immune recognition. Journal of Biological Chemistry 278, 16941–16951.CrossRefGoogle ScholarPubMed
Zhan, B., Hotez, P.J., Wang, Y. & Hawdon, J.M. (2002) A developmentally regulated metalloprotease secreted by host-stimulated Ancylostoma caninum third-stage infective larvae is a member of the astacin family of proteases. Molecular and Biochemical Parasitology 120, 291–296.CrossRefGoogle ScholarPubMed