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Anthelmintic effects of Prosopis laevigatan-hexanic extract against Haemonchus contortus in artificially infected gerbils (Meriones unguiculatus)

Published online by Cambridge University Press:  21 July 2009

A.F. De Jesús-Gabino ,
P. Mendoza-de Gives ,
D.O. Salinas-Sánchez ,
M.E. López-Arellano ,
E. Liébano-Hernández ,
V.M. Hernández-Velázquez  and
G. Valladares-Cisneros
A.F. De Jesús-Gabino
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
P. Mendoza-de Gives*
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
D.O. Salinas-Sánchez
Affiliation:
FCB, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México CEAMISH, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México
M.E. López-Arellano
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
E. Liébano-Hernández
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
V.M. Hernández-Velázquez
Affiliation:
CEIB, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México
G. Valladares-Cisneros
Affiliation:
FCQI, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México
*
*Fax: (+52) 73 192850 Ext. 129 E-mail: pedromdgives@yahoo.com
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Abstract

The anthelmintic effect of Prosopis laevigata (mezquite) n-hexanic extract was evaluated against Haemonchus contortus endoparasitic stages in artificially infected gerbils (Meriones unguiculatus). Prosopis laevigata leaves were collected from the Sierra de Huautla, Ecological Reserve of the Biosphere, in Morelos State, Mexico; dehydrated under shade and macerated with n-hexane for 3 days, followed by distillation for 8 h. This procedure was repeated three times and the final extract was kept at 4°C. The in vivo effect of the plant extract was evaluated in gerbils artificially infected with H. contortus. Plant extract concentration was 40 mg/ml. Three groups of gerbils were as follows: group 1 (n = 7), P. laevigata extract at 100 μl intraperitoneally (IP); group 2 (n = 6), control – Tween 20 in water at a single dose of 100 μ l IP; group 3 (n = 8) also served as a control, receiving water only, to determine the mortality due to causes other than the plant extract. An additional group of seven gerbils (group 4) was administered fenbendazole, as a positive control. Five days later the animals were euthanized and stomach and mucosa removed to quantify the nematodes. Data were analysed using the Student's t-test to compare the mean of nematodes obtained in groups 1, 2 and 3. The parasite population in the plant extract treated group 1 was reduced by 42.5% (P < 0.05) with respect to the control group 2; and when control group 3 was used for comparison the parasitic reduction was estimated as 53.11%. This study shows the in vivo anthelmintic effect of P. laevigatan-hexane extract for the first time, using gerbils as an in vivo model, with potential use in sheep.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 1 , March 2010 , pp. 71 - 75
Copyright
Copyright © Cambridge University Press 2009

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