Skip to main content Accessibility help
×
Home

Analysis of excretory–secretory and somatic antigens of Gastrothylax crumenifer

  • M.K. Saifullah (a1), Gul Ahmad (a1) and W.A. Nizami (a1)

Abstract

The excretory/secretory (ES) metabolic products released by Gastrothylax crumenifer (Trematoda: Digenea) during in vitro incubations and the somatic extract of the adult parasite were analysed using polyacrylamide gel electrophoresis (PAGE). Immunogenicity of ES and somatic extracts were evaluated by immunoblotting and ELISA using sera raised against ES and somatic antigens in rabbits. The electropherograms of ES and somatic extracts have been resolved into 38 and 41 polypeptides, respectively. The apparent molecular weights of these polypeptides range from < 29 to > 205 kDa. A total of 14 polypeptides were found to be common to both of the samples. The analysis of immunoblot results revealed 22 and 27 antigenic polypeptides in ES and somatic extracts respectively. Only 11 and 13 antigenic polypeptides were found specific to ES and tissue extract respectively. The molecular weights of these specific polypeptides were calculated to be < 14.4, 16, 20, 25, 33, 42, 119, 125 and > 205 kDa for metabolic products and < 14.4, 25, 30, 35, 78, 84 and > 205 kDa for the tissue extracts, respectively. Analysis of ELISA results revealed that a dilution of up to 1:3200 of the test sera could react with the ES product. Further, when the ES antigens were allowed to react with antisomatic extracts in hyperimmune sera the titre of IgG increased up to a dilution of 1:12800. The potential importance of these antigens in the immunodiagnosis of amphistomiasis is discussed.

Copyright

Corresponding author

*Author for correspondence. Fax +91 0571 400637 E-mail zyr015@amu.up.nic.in

References

Hide All
Bawa, H.S.B. (1939) Intestinal paramphistomiasis of sheep in Sind (a preliminary report). Indian Journal of Veterinary Science and Animal Husbandry 9, 425429.
Dutt, S.C. (1980) Paramphistomes and paramphistomiasis of domestic ruminants in India. Ludhiana, Punjab Agricultural University Press.
Eduardo, S.L. (1985) The taxonomy of the family Paramphistomidae Fischoeder, 1901 with special to the morphology of species occurring in ruminants. VI. Revision of the genus Orthocoelium (Stiles & Goldberger, 1910) Price & McIntosh, 1953. Systematic Parasitology 7, 125158.
Hanna, R.E.B., Williamson, D.S., Mattison, R.G. & Nizami, W.A. (1988) Seasonal reproduction in Paramphistomum epiclitum and Gastrothylax crumenifer, rumen amphistomes of the Indian water buffalo and comparison with the bilary paramphistome Gigantocotyle explanatum. International Journal for Parasitology 18, 513521.
Harlow, E. & Lane, D. (1988) Antibodies — a laboratory manual. Cold Spring Harbor Laboratory, USA.
Hillyer, G.V. & Serrano, A.E. (1983) The antigens of Paragonimus westermani, Schistosoma mansoni and Fasciola hepatica adult worms. Evidence for the presence of cross-reactive antigens and for cross protection to Schistosoma mansoni using antigens of Paragonimus westermani. American Journal of Tropical Medicine and Hygiene 32, 350358.
Horak, I.G. (1971) Paramphistomiasis of domestic ruminants. Advances in Parasitology 9, 3372.
Katiyar, R.D. & Varshney, T.R. (1963) Amphistomiasis in sheep and goats in Uttar Pradesh. Indian Journal of Veterinary Science and Animal Husbandry 33, 9498.
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T. Nature 227, 680685.
Maleewong, W., Wongkham, C., Intpan, P., Pariyanonda, S. & Movrakote, N. (1992) Excretory-secretory antigenic components of Paragonimus heterotremus recognized by infected human sera. Journal of Clinical Microbiology 30, 20772079.
Mattison, R.G., Abidi, S.M.A., Nizami, W.A. & Hanna, R.E.B. (1994) Preliminary studies in numerical systematics of the Paramphistomatoidea (Digenea) from domestic Artiodactyla of Northern India. Systematic Parasitology 27, 81103.
Mehra, H.R. (1980) A fauna of India and adjacent countries. Platyhelminthes Vol. Trematoda. Calcutta, Zoological Survey of India (Publ.).
Nasmark, K.E. (1937) A revision of the trematode family Paramphistomoidae. Zoology Bidrag Fran Uppsala 16, 301566.
Oakley, B.R., Kirsch, D.R. & Morris, N.R. (1980) A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105, 361363.
Panda, B.K. & Misra, S.C. (1980) Observations on the etiology, clinical pathology and chemotherapy of immature amphistomiasis in buffalo calves. Indian Journal of Animal Health 19, 131135.
Pande, P.G. (1935) Acute amphistomiasis of cattle in Assam: a preliminary report. Indian Journal of Veterinary Science and Animal Husbandry 5, 364375.
Paron, D., Setasuban, P., Waikagul, J., Yaemput, S. & Sanguankiat, S. (1995) Human lung fluke Paragonimus heterotremus: differentiation of antigenic protein of adult worms by enzyme-linked immunoelectrotransfer blot technique. Southeast Asian Journal of Tropical Medicine and Public Health 26, 434438.
Sey, O. (1979) Examination of the validity and systematic position of some paramphistomids of Indian ruminants. Parasitologia Hungarica 12, 3136.
Spector, T. (1978) Refinement of the Coomassie Brilliant blue method for protein quantitation. Analytical Biochemistry 86, 142146.
Stiles, C.W. & Goldberger, J. (1910) A study of the anatomy of Watsonius (n.g.) watsoni of man and of nineteen allied species of mammalian trematode worms of the superfamily Paramphistomatoidae. Bulletin of the Hygienic Laboratory, Public Health and Marine-Hospital Service of the United States, No. 60, 259.
Thapar, G.S. (1956) Systematic survey of helminth parasites of domesticated animals in India. Indian Journal of Veterinary Science and Animal Husbandry 26, 211271.
Towbin, H., Staehelin, T. & Godon, J. (1979) Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences USA 76, 43504354.

Analysis of excretory–secretory and somatic antigens of Gastrothylax crumenifer

  • M.K. Saifullah (a1), Gul Ahmad (a1) and W.A. Nizami (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed