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An evaluation of antigen capture assays for detecting active filarial antigens

Published online by Cambridge University Press:  02 April 2014

R. Ravishankaran ,
N.S. Radhika ,
L. Ansel Vishal ,
S. Meenakshisundaram ,
A.A. Karande  and
P. Kaliraj
R. Ravishankaran
Affiliation:
Center for Biotechnology, Anna University, Chennai, Tamil Nadu, India
N.S. Radhika
Affiliation:
Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India
L. Ansel Vishal
Affiliation:
Center for Biotechnology, Anna University, Chennai, Tamil Nadu, India
S. Meenakshisundaram
Affiliation:
Center for Biotechnology, Anna University, Chennai, Tamil Nadu, India
A.A. Karande*
Affiliation:
Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India
P. Kaliraj*
Affiliation:
Center for Biotechnology, Anna University, Chennai, Tamil Nadu, India
*
*Fax: +91 80 2360 0814 (A.A.K) E-mail: anjali@biochem.iisc.ernet.in; Fax: +91 044-22350299 (P.K.) E-mail: pkaliraj@annauniv.edu
*Fax: +91 80 2360 0814 (A.A.K) E-mail: anjali@biochem.iisc.ernet.in; Fax: +91 044-22350299 (P.K.) E-mail: pkaliraj@annauniv.edu
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Abstract

Lymphatic filariasis is a parasitic disease of tropical countries. This is a disfiguring and painful disease contracted in childhood, but the symptoms become apparent only in later years. Diagnosis of filarial infection is very crucial for the management of the disease. The main objective of this study was to develop a filarial antigen-based immunological assay for the diagnosis and surveillance of the disease. Monoclonal and polyclonal antibodies were raised to the recombinant protein Brugia malayi vespid allergen homologue (VAH). Capture enzyme-linked immunosorbent assay (ELISA) was standardized utilizing various combinations of antibodies and evaluated with serum samples of endemic normal (EN, n= 110), microfilaraemic (MF, n= 65), chronic pathology (CP, n= 45) and non-endemic normal (NEN, n= 10) individuals. Of the 230 samples tested, VAH capture assay detected circulating antigen in 97.91% of bancroftian and 100% of brugian microfilaraemic individuals, and 5% of endemic normal individuals, comparable to the earlier reported SXP-1 antigen detection assay. However, the combination of VAH and SXP-1 (VS) capture ELISA was found to be more robust, detecting 100% of microfilaraemic individuals and with higher binding values. Thus an antigen capture immunoassay has been developed, which can differentiate active infection from chronic infection by detecting circulating filarial antigens in clinical groups of endemic areas.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 3 , May 2015 , pp. 352 - 358
Copyright
Copyright © Cambridge University Press 2014 

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