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Molecular and morphological evidence for the occurrence of Anisakis sp. A (Nematoda, Anisakidae) in the Blainville's beaked whale Mesoplodon densirostris

Published online by Cambridge University Press:  01 December 2008

R. Iglesias
Affiliation:
Laboratory of Parasitology, Department of Functional Biology and Health Sciences, Faculty of Biology, University of Vigo, Lagoas-Marcosende s/n, 36310Vigo, Spain
S. D'Amelio
Affiliation:
Department of Sciences of Public Health, Section of Parasitology, Sapienza University of Rome, P.le Aldo Moro 5, 00185Rome, Italy
S. Ingrosso
Affiliation:
Department of Sciences of Public Health, Section of Parasitology, Sapienza University of Rome, P.le Aldo Moro 5, 00185Rome, Italy
S. Farjallah
Affiliation:
Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir5000, Tunisie
J.A. Martínez-Cedeira
Affiliation:
Coordinadora para o Estudo dos Mamíferos Mariños (CEMMA), Apdo. 165, 36380Gondomar (Pontevedra), Spain
J.M. García-Estévez
Affiliation:
Laboratory of Parasitology, Department of Functional Biology and Health Sciences, Faculty of Biology, University of Vigo, Lagoas-Marcosende s/n, 36310Vigo, Spain
Corresponding
E-mail address:

Abstract

Twenty-three adults (only one male) and two fourth-stage larvae of Anisakis, recovered from the stomach of a Blainville's beaked whale (Mesoplodon densirostris) stranded in Galicia (NW Spain), were studied morphologically and molecularly. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing studies carried out on the ribosomal DNA spacers and on the cox2 mitochondrial gene confirm that these nematodes belong to the taxon Anisakis sp. A, which was previously detected as a larval stage in fishes from Madeiran waters. In addition, our molecular studies demonstrate that Anisakis sp. A and the taxon Anisakis sp., previously found in other Mesoplodon spp., are the same species. The adults of Anisakis sp. A are morphologically similar to A. ziphidarum but with a shorter body length and longer spicules (right spicule 2.42 mm, left spicule 2.30 mm). Since the poor condition of the male tail cuticle prevented a proper description of the caudal plates and the pattern of caudal papillae, we propose to retain the name Anisakis sp. A until new males are described correctly.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2008

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References

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Molecular and morphological evidence for the occurrence of Anisakis sp. A (Nematoda, Anisakidae) in the Blainville's beaked whale Mesoplodon densirostris
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Molecular and morphological evidence for the occurrence of Anisakis sp. A (Nematoda, Anisakidae) in the Blainville's beaked whale Mesoplodon densirostris
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