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Characteristics of DNase activities in excretory/secretory products of infective larvae of Haemonchus contortus

Published online by Cambridge University Press:  30 August 2011

S.H. Yun
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
M.G. Seo
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
B.Y. Jung
Affiliation:
Animal Disease Diagnostic Centre, National Veterinary Research and Quarantine Service, 335 Joongangro, Anyang, Kyunggi430-824, Republic of Korea
T.H. Kim
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
O.D. Kwon
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
K.S. Jeong
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
M.H. Rhee
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
Y.J. Lee
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
S.J. Park
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
Y.S. Kwon
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
D. Kwak
Affiliation:
College of Veterinary Medicine, Kyungpook National University, 80 Daehakno, Bukgu, Daegu702-701, Republic of Korea
Corresponding
E-mail address:

Abstract

While multiple DNase activities occur in the excretory/secretory products (ESPs) of the adult Haemonchus contortus, the DNase activities in ESPs of the infective larvae (L3) have not been studied. Thus, the DNase activities in ESPs of H. contortus L3 were investigated and compared to those of adults for developmental stage-specific analysis. The DNase activities had relative molecular masses (Mrs) of 34 and 36 kDa upon zymographic analysis at pH 5.0 and 7.0 when the larvae were incubated for over 48 h. The 34 and 36 kDa DNases of L3 ESPs were also detected in adult ESPs with similar characteristics. However, the 37 and 38.5 kDa DNases of the adult ESPs were not detected in the L3 ESPs. Since the 37 and 38.5 kDa DNase activities were mainly detected in adult ESPs, these activities appear to be specific to the adult stage whereas the other ESP DNase activities appear to be expressed during multiple stages of the parasite's life cycle. While the difference in DNase activities of L3 and adults remains obscure, the role of DNase in larval development should be further clarified and the identification of stage-specific developmental markers will lead to the discovery of specific factors that stimulate larval development.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2011

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References

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