Skip to main content Accessibility help
×
Home

Deformation of DNA molecules by hydrodynamic focusing

  • PAK KIN WONG (a1), YI-KUEN LEE (a2) and CHIH-MING HO (a1)

Abstract

The motion of a DNA molecule in a solvent flow reflects the deformation of a nano/microscale flexible mass–spring structure by the forces exerted by the fluid molecules. The dynamics of individual molecules can reveal both fundamental properties of the DNA and basic understanding of the complex rheological properties of long-chain molecules. In this study, we report the dynamics of isolated DNA molecules under homogeneous extensional flow. Hydrodynamic focusing generates homogeneous extensional flow with uniform velocity in the transverse direction. The deformation of individual DNA molecules in the flow was visualized with video fluorescence microscopy. A coil–stretch transition was observed when the Deborah number (De) is larger than 0.8. With a sudden stopping of the flow, the DNA molecule relaxes and recoils. The longest relaxation time of T2 DNA was determined to be 0.63 s when scaling viscosity to 0.9 cP.

Copyright

MathJax
MathJax is a JavaScript display engine for mathematics. For more information see http://www.mathjax.org.

Deformation of DNA molecules by hydrodynamic focusing

  • PAK KIN WONG (a1), YI-KUEN LEE (a2) and CHIH-MING HO (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed