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Extended light period in the maternal circadian cycle impairs the reproductive system of the rat male offspring

Published online by Cambridge University Press:  28 October 2020

Fernanda Mithie Ogo ,
Glaucia Eloisa Munhoz Lion Siervo ,
Ana Maria Praxedes de Moraes ,
Katia Gama de Barros Machado ,
Suellen Ribeiro da Silva Scarton ,
Ana Tereza Bittencourt Guimarães ,
Alessandra Lourenço Cecchini ,
Andréa Name Colado Simão ,
Paulo Cezar de Freitas Mathias Open the ORCID record for Paulo Cezar de Freitas Mathias [Opens in a new window]  and
Glaura Scantamburlo Alves Fernandes Open the ORCID record for Glaura Scantamburlo Alves Fernandes [Opens in a new window]
Fernanda Mithie Ogo
Affiliation:
Department of General Biology, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil Department of Pathological Sciences, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil
Glaucia Eloisa Munhoz Lion Siervo
Affiliation:
Department of General Biology, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil Department of Pathological Sciences, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil
Ana Maria Praxedes de Moraes
Affiliation:
Department of Biotechnology, Genetics and Cell Biology, State University of Maringá – UEM, Maringá, Paraná, Brazil
Katia Gama de Barros Machado
Affiliation:
Department of Biotechnology, Genetics and Cell Biology, State University of Maringá – UEM, Maringá, Paraná, Brazil
Suellen Ribeiro da Silva Scarton
Affiliation:
Department of General Biology, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil Department of Pathological Sciences, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil
Ana Tereza Bittencourt Guimarães
Affiliation:
Department of Biological and Health Sciences, State University of Western Parana, Cascavel, Paraná, Brazil
Alessandra Lourenço Cecchini
Affiliation:
Department of Pathological Sciences, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil
Andréa Name Colado Simão
Affiliation:
Department of Pathology, Clinical Analysis and Toxicology, Health Center, State University of Londrina – UEL, Londrina, Paraná, Brazil
Paulo Cezar de Freitas Mathias
Affiliation:
Department of Biotechnology, Genetics and Cell Biology, State University of Maringá – UEM, Maringá, Paraná, Brazil
Glaura Scantamburlo Alves Fernandes*
Affiliation:
Department of General Biology, Biological Sciences Center, State University of Londrina – UEL, Londrina, Paraná, Brazil
*
Address for correspondence: Glaura Scantamburlo Alves Fernandes, Department of General Biology, State University of Londrina (UEL), RodoviaCelso Garcia Cid, Pr 445, Km 380, 86057-970, Londrina, Paraná, Brazil. Email: glaura@uel.br
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Abstract

Alterations in the circadian cycle are known to cause physiological disorders in the hypothalamic–pituitary–adrenal and the hypothalamic–pituitary–gonadal axes in adult individuals. Therefore, the present study aimed to evaluate whether exposure of pregnant rats to constant light can alter the reproductive system development of male offspring. The dams were divided into two groups: a light–dark group (LD), in which pregnant rats were exposed to an LD photoperiod (12 h/12 h) and a light–light (LL) group, in which pregnant rats were exposed to a photoperiod of constant light during the gestation period. After birth, offspring from both groups remained in the normal LD photoperiod (12 h/12 h) until adulthood. One male of each litter was selected and, at adulthood (postnatal day (PND) 90), the trunk blood was collected to measure plasma testosterone levels, testes and epididymis for sperm count, oxidative stress and histopathological analyses, and the spermatozoa from the vas deferens to perform the morphological and motility analyses. Results showed that a photoperiod of constant light caused a decrease in testosterone levels, epididymal weight and sperm count in the epididymis, seminiferous tubule diameter, Sertoli cell number, and normal spermatozoa number. Histopathological damage was also observed in the testes, and stereological alterations, in the LL group. In conclusion, exposure to constant light during the gestational period impairs the reproductive system of male offspring in adulthood.

Keywords

Circadian cyclegestationdevelopmentmale reproductive systemadulthood
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 4 , August 2021 , pp. 595 - 602
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Copyright
© The Author(s), 2020. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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