Skip to main content Accessibility help
×
Home

A single nucleotide polymorphism in the promoter region of river buffalo stearoyl CoA desaturase gene (SCD) is associated with milk yield

  • Alfredo Pauciullo (a1), Gianfranco Cosenza (a1), Roberto Steri (a2), Angelo Coletta (a3), Antonio La Battaglia (a4), Dino Di Berardino (a1), Nicolò P P Macciotta (a2) and Luigi Ramunno (a1)...

Abstract

An association study between the milk yield trait and the stearoyl-CoA desaturase (SCD) polymorphism (g.133A > C) in Italian Mediterranean river buffalo was carried out. A full characterization of the river buffalo SCD promoter region was presented. Genotyping information was provided and a quick method for allelic discrimination was developed. The frequency of the C allele was 0·16. Test-day (TD) records (43 510) of milk production belonging to 226 lactations of 169 buffalo cows were analysed with a mixed linear model in order to estimate the effect of g.133A > C genotype, as well as the effect of parity and calving season. The SCD genotype was significantly associated with milk yield (P = 0·02). The genotype AC showed an over-dominance effect with an average daily milk yield approximately 2 kg/d higher than CC buffaloes. Such a difference represents about 28% more milk/d. The effect of the genotype was constant across lactation stages. The contribution of SCD genotype (r2SCD) to the total phenotypic variance in milk yield was equal to 0·12. This report is among the first indications of genetic association between a trait of economic importance in river buffalo. Although such results need to be confirmed with large-scale studies in the same and other buffalo populations, they might offer useful indications for the application of MAS programmes in river buffalo and in the future they might be of great economic interest for the river buffalo dairy industry.

Copyright

Corresponding author

*For correspondence; e-mail: apauciul@unina.it; giacosen@unina.it

References

Hide All
Afzal, M, Anwar, M & Mirza, MA 2007 Some factors affecting milk yield and lactation length in Nili-Ravi buffaloes. Pakistan Veterinary Journal 27 113117
AIA 2010 Milk recording activity: official statistics. In Annual Report of the Italian Breeders Association. p. 31. Rome, Italy: Associazione Italiana Allevatori
Banos, G, Woolliams, JA, Woodward, BW, Forbes, AB & Coffey, MP 2008 Impact of single nucleotide polymorphisms in leptin, leptin receptor, growth hormone receptor, and diacylglycerol acyltransferase (DGAT1) gene loci on milk production, feed, and body energy traits of UK dairy cows. Journal of Dairy Science 91 31903200
Barile, VL 2005 Improving reproductive efficiency in female buffaloes. Livestock Production Science 92 183194
Bionaz, M & Loor, JJ 2008 Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics 9 366
Borghese, A & Mazzi, M 2005 Buffalo population and strategies in the world. In Buffalo Production and Research, pp. 140. Roma, Italy: FAO – Regional Office for Europe
Carta, A, Casu, S, Usai, MG, Addis, M, Fiori, M, Fraghı, A, Miari, S, Mura, L, Piredda, G, Schibler, L, Sechi, T, Elsen, JM & Barillet, F 2008 Investigating the genetic component of fatty acid content in sheep milk. Small Ruminant Research 79 2228
Catillo, G, Macciotta, NPP, Carretta, A & Cappio-Borlino, A 2002 Effects of age and calving season on lactation curves of milk production traits in Italian water buffaloes. Journal of Dairy Science 85 12981306
Dang, AK, Mukherjee, J, Kapila, S, Mohanty, AK, Kapila, R & Prasad, S 2010 In vitro phagocytic activity of milk neutrophils during lactation cycle in Murrah buffaloes of different parity. Journal of Animal Physiology and Animal Nutrition 94 706711
Drost, M 2007 Advanced reproductive technology in the water buffalo. Theriogenology 68 450453
García-Fernández, M, Gutiérrez-Gil, B, García-Gámez, E, Sánchez, JP & Arranz, JJ 2010 Detection of quantitative trait loci affecting the milk fatty acid profile on sheep chromosome 22: role of the stearoyl-CoA desaturase gene in Spanish Churra sheep. Journal of Dairy Science 93 348357
Gautier, M, Barcelona, RR, Fritz, S, Grohs, C, Druet, T, Boichard, D, Eggen, A & Meuwissen, THE 2006 Fine mapping and physical characterization of two linked quantitative trait loci affecting milk fat yield on BTA26. Genetics 172 425436
Gossens, M & Kan, JW 1981 DNA analysis in the diagnosis of haemoglobin disorders. Methods in Enzymology 76 805817
Graves, RA, Tontonoz, P, Ross, SR & Spiegelman, BM 1991 Identification of a potent adipocyte-specific enhancer: involvement of an NF-1-like factor. Genes and Development 5 428437
Grisart, B, Coppieters, W, Farnir, F, Karim, L, Ford, C, Berzi, P, Cambisano, N, Mni, M, Reid, S, Simon, P, Spelman, R, Georges, M & Snell, R 2002 Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research 12 222231
Harvatine, KJ & Bauman, DE 2006 SREBP1 and thyroid hormone responsive spot 14 (S14) are involved in the regulation of bovine mammary lipid synthesis during diet-induced milk fat depression and treatment with CLA. Journal of Nutrition 136 24682474
Kay, JK, Weber, WJ, Moore, CE, Bauman, DE, Hansen, LB, Chester-Jones, H, Crooker, BA & Baumgard, LH 2005 Effect of week of lactation and genetic selection for milk yield on milk fatty acid composition in Holstein cows. Journal of Dairy Science 88 38863893
Kuehn, C, Edel, C, Weikard, R & Thaller, G 2007 Dominance and parent-of-origin effects of coding and non-coding alleles at the acylCoA-diacylglycerol-acyltransferase (DGAT1) gene on milk production traits in German Holstein cows. BMC Genetics 24 62
Littell, RC, Henry, PR & Ammerman, CB 1998 Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76 12161231
Macciotta, NPP, Mele, M, Conte, G, Serra, A, Cassandro, M, Dal Zotto, R, Cappio, Borlino A, Pagnacco, G & Secchiari, P 2008 Association between a polymorphism at the stearoyl CoA desaturase locus and milk production traits in Italian Holsteins. Journal of Dairy Science 91 31843189
Mele, M, Conte, G, Castiglioni, B, Chessa, S, Macciotta, NP, Serra, A, Buccioni, A, Pagnacco, G & Secchiari, P 2007 Stearoyl-coenzyme A desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. Journal of Dairy Science 90 44584465
Moioli, B, Contarini, G, Avalli, A, Catillo, G, Orru, L, De Matteis, G, Masoero, G & Napolitano, F 2007 Effect of stearoyl-coenzyme A desaturase polymorphism on fatty acid composition of milk. Journal of Dairy Science 90 35533558
Ntambi, JM 1999 Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of Lipid Research 40 15491558
Olofsson, LE, Orho-Melander, M, William-Olsson, L, Sjöholm, K, Sjöström, L, Groop, L, Carlsson, B, Carlsson, LM & Olsson, B 2008 CCAAT/enhancer binding protein α (C/EBPα) in adipose tissue regulates genes in lipid and glucose metabolism and a genetic variation in C/EBPα is associated with serum levels of triglycerides. Journal of Clinical Endocrinology and Metabolism 93 48804886
Paton, CM & Ntambi, JM 2009 Biochemical and physiological function of stearoyl-CoA desaturase. American Journal of Physiology – Endocrinology and Metabolism 297 E28E37
Pauciullo, A, Cosenza, G, D'Avino, A, Colimoro, L, Nicodemo, D, Coletta, A, Feligini, M, Marchitelli, C, Di Berardino, D & Ramunno, L 2010 Sequence analysis and genetic variability of stearoyl CoA desaturase (SCD) gene in the Italian Mediterranean river buffalo. Molecular and Cellular Probes 24 407410
Pauciullo, A, Cosenza, G, Steri, R, Coletta, A, Jemma, L, Feligini, M, Di Berardino, D, Macciotta, NPP & Ramunno, L 2011 An association analysis between OXT genotype and milk yield and flow in Italian Mediterranean river buffalo. Journal of Dairy Research 79 150156
Stanton, TL, Jones, LR, Everett, RW & Kachman, SD 1992 Estimating milk, fat, and protein lactation curves with a test day model. Journal of Dairy Science 75 16911700
Zhu, J, He, F, Hu, S & Yu, J 2008 On the nature of human housekeeping genes. Trends in Genetics 24 481484

Keywords

A single nucleotide polymorphism in the promoter region of river buffalo stearoyl CoA desaturase gene (SCD) is associated with milk yield

  • Alfredo Pauciullo (a1), Gianfranco Cosenza (a1), Roberto Steri (a2), Angelo Coletta (a3), Antonio La Battaglia (a4), Dino Di Berardino (a1), Nicolò P P Macciotta (a2) and Luigi Ramunno (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed