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Simple methods for the purification of crude κ-casein and β-casein by treatment with calcium phosphate gel

Published online by Cambridge University Press:  01 June 2009

Margaret L. Green
Margaret L. Green
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
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Summary

Batch methods applicable on a large scale are described for the purification of crude κ- and βκ-Casein, dissolved in urea-containing buffer, was freed from αs- and β-caseins by treatment with calcium phosphate gel and recovered in about 60% yield. β-Casein was freed from most impurities by adsorption on to calcium phosphate gel at pH 7·8 in the presence of urea and elution with 6 M-urea–N-NH4OH at 4°C. The recovery was about 50%.

Type
Original Articles
Information
Journal of Dairy Research , Volume 36 , Issue 3 , October 1969 , pp. 353 - 359
Copyright
Copyright © Proprietors of Journal of Dairy Research 1969

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References

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