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Proteinase, peptidase and esterase activities of cell-free extracts from wild strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus salivarius subsp. thermophilus isolated from traditional Greek yogurt

Published online by Cambridge University Press:  01 June 2009

Georgios Kalantzopoulos
Affiliation:
Laboratory of Dairy Technology, Agricultural University of Athens, Botanikos, 118 55 Athens, Greece
Efthimia Tsakalidou
Affiliation:
Laboratory of Dairy Technology, Agricultural University of Athens, Botanikos, 118 55 Athens, Greece
Eugenia Manolopoulou
Affiliation:
Laboratory of Dairy Technology, Agricultural University of Athens, Botanikos, 118 55 Athens, Greece

Summary

Proteinase, peptidase and esterase activities were detected in cell-free extracts of four Lactobacillus delbrueckii subsp. bulgaricus and four Streptococcus salivarius subsp. thermophilus strains. Post-electrophoretic detection was based on hydrolysis of L-leucine-βnaphthylamide and α-naphthylacetate. The substrates L-leucine-p–nitroanilide, N-acetyl-L-alanine-p–nitroanilide, 2-nitrophenylbutyrate and 4-nitrophenylbutyrate were used for the spectrophotometric detection of the enzymes. Estimation of total proteolytic activity was based on hydrolysis of bovine whole casein. Interesting differences were observed between the two French strains and those isolated from traditional Greek yogurt.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1990

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