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On the size of monomers and polymers of β-casein

Published online by Cambridge University Press:  01 June 2009

Wolfgang Buchheim  and
Daniël G. Schmidt
Wolfgang Buchheim
Affiliation:
Institute for Physics, Federal Dairy Research Centre, D2300 Kiel, Federal Republic of Germany
Daniël G. Schmidt
Affiliation:
Netherlands Institute for Dairy Research, Ede, The Netherlands
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Summary

The size and number average molecular weight have been determined for β-casein monomers and polymers from electron micrographs using the freeze-etching procedure with spray-frozen specimens. For the spherical β-casein monomers we found a mol. wt of 22600 and a diam. about 10 nm, which compared quite well with data obtained from ultracentrifugation, light scattering and viscosity measurements. Polymer sizes were in agreement with molecular weight determinations from ultracentrifugation and light scattering, assuming that the volume and weight of the particles are proportional.

Type
Section C. Association of Proteins
Information
Journal of Dairy Research , Volume 46 , Issue 2: Symposium on the Physics and Chemistry of Milk Proteins , April 1979 , pp. 277 - 280
Copyright
Copyright © Proprietors of Journal of Dairy Research 1979

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