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Occurrence of five αs1-casein variants in ovine milk

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Giuseppina Garro ,
Rosalba Mauriello ,
Pasquale Laezza ,
Pasquale Ferranti  and
Francesco Addeo
Lina Chianese
Affiliation:
Dipartimento di Scienza degli Alimenti, Universitá degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Giuseppina Garro
Affiliation:
Dipartimento di Scienza degli Alimenti, Universitá degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Rosalba Mauriello
Affiliation:
Dipartimento di Scienza degli Alimenti, Universitá degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Pasquale Laezza
Affiliation:
Dipartimento di Scienza degli Alimenti, Universitá degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Pasquale Ferranti
Affiliation:
Dipartimento di Scienza degli Alimenti, Universitá degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Francesco Addeo
Affiliation:
Istituto Sperimentale Lattiero-Caseario, Via A. Lombardo 11, 1-20075 Lodi, Italia
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Summary

Five ovine αsl-casein variants (A–E) were identified in an Italian population sample using gel electrophoresis at alkaline pH, gel isoelectric focusing, two dimensional gel electrophoresis, and immunoblotting with polyclonal antibodies against αs1-casein. Each casein sample produced two peaks by fast reversed-phase HPLC. Gel isoelectric focusing and electrospray mass spectrometry were used to demonstrate that the first HPLC peak contained the 191 residue αsl-casein molecular species and the second the 199 residue species, in proportions of ∼20:80. Only in the case of the sample containing αsl-casein CE was the method for the separation of the single short and long forms of each variant unsuccessful. Both two dimensional electrophoresis followed by staining with polyclonal antibodies against αsl-casein and electrospray mass spectrometry showed a heterogeneity consistent with that expected from a protein chain with three levels of phosphorylation and two different lengths. However, especially for αsl-caseins D and E, a further uncharacterized heterogeneity was detected.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 1 , February 1996 , pp. 49 - 59
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

REFERENCES

Addeo, F., Mauriello, R., Moio, L., Laezza, P., Chianese, L. & Di Luccia, A. 1992 Ovine casein variant identification using electrophoretic, immunochemical and chromatographic techniques. Milchwissenschafi 47 283–287Google Scholar
Aschakfenburg, R. & Drewry, J. 1959 New procedure for the routine determination of the various non-casein proteins of milk. 15th International Dairy Congress, London 3 1631–1637Google Scholar
Bingham, E. W., Farrell, H. M. & Carroll, R. J. 1972 Properties of dephosphorylated αsl-casein. Precipitation by calcium ions and micelle formation. Biochemistry 11 2450–2454CrossRefGoogle Scholar
Boisnard, M., Hue, D., Bouniol, C., Mercier, J. -C. & Gaye, P. 1991 Multiple mRNA species code for two non-allelic forms of ovine αs2-casein. European Journal of Biochemistry 201 633–641Google Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1992 Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques. Journal of Dairy Research 59 39–47CrossRefGoogle ScholarPubMed
Chianese, L., Mauriello, R., Moio, L., Intorcia, N., Campus, R. & Addeo, F. 1990 [Casein characterization in the Sarda ovine breed.] Proceedings of the XLIVth S. I. S.Vet. (Italian Society of Veterinary Science) National Congress, Stresa 44 1701–1704Google Scholar
Di Luccia, A., Iannibelli, L., Ferrara, L., Ledda, L., Moio, L., Pieragostini, E. & Addeo, F. 1989 Identification of Welsh variant from ovine casein by electrophoresis techniques. In Electrophoresis Forum ‚89, pp. 394–397 (Ed. Radola, B. J.). MünchenGoogle Scholar
Ferranti, P., Malorni, A., Nitti, G., Laezza, P., Pizzano, R., Chianese, L. & Addeo, F. 1995 Primary structure of ovine αsl-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D. Journal of Dairy Research 62 281–296CrossRefGoogle ScholarPubMed
Grosclaude, F., Rioordeau, G., Martin, P., Remeuf, F., Vassal, L. & Bouillon, J. 1994 [From gene to cheese: the αsl-casein polymorphism in goat, its effect and evolution.] INRA Production Animale 7 3 19Google Scholar
King, J. W. B. 1967 The caseins of sheep's milk. In Polymorphismes Biochimiques des Animaux pp. 427–431. Paris: INRA (European Conference on Animal Blood Groups and Biochemical Polymorphisms no. 10 1966)Google Scholar
Krause, I., Buchberger, J., Weiss, G., Pflugler, M. & Klostermeyer, H. 1988 Isoelectric focusing in immobilized pH gradients with carrier ampholytes added for high-resolution phenotyping of bovine β-lactoglobulins: characterization of a new genetic variant. Electrophoresis 9 609–613CrossRefGoogle ScholarPubMed
Mauriello, R., Pieragostini, E., Bufano, G. & Addeo, F. 1990 [Casein polymorphism in the Altamurana sheep breed.] Scienza e Tecnica Lattiero-Casearia 41 357–364Google Scholar
Pieragostini, E., Chianese, L., Mauriello, R. & D'acierno, C. 1989 [Polymorphism of caseins in Leccese sheep.] Proceedings of the XLIIIrd S. 1. S. Vet. (Italian Society of Veterinary Science) National Congress, Pisa 43 1875–1879Google Scholar
Piredda, G., Papoff, C. M., Sauna, S. R. & Campus, R. L. 1993 [Influence of ovine αsl-easein genotype upon the physicochemical and lactodynamographic characteristics of the milk.] Scienza e Tecnica Lattiero–Casearia 44 135–143Google Scholar
Rolland, M. -P., Bitri, L. & Besançon, P. 1993 Polyclonal antibodies with predetermined specificity against bovine αsl-casein: application to the detection of bovine milk in ovine milk and cheese. Journal of Dairy Research 60 413–420CrossRefGoogle Scholar
Rolland, M. P., Bitri, L. & Besançon, P. 1995 Monospecificity of the antibodies to bovine αsl-casein fragment 140 149: application to the detection of bovine milk in caprine dairy products. Journal of Dairy Research 62 83–88CrossRefGoogle Scholar
Sadler, A. M., Kiddy, C. A., McCann, R. E. & Mattingly, W. A. 1968 Acid production and curd toughness in milks of different αsl-casein types. Journal of Dairy Science 51 28–30CrossRefGoogle Scholar
Van Hekken, D. L. & Strange, E. D. 1993 Functional properties of dephosphorylated bovine whole casein. Journal of Dairy Science 76 3384–3391CrossRefGoogle Scholar