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New αs2-casein variant from caprine milk

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Rosalba Mauriello ,
Nunziatina Intorcia ,
Luigi Moio  and
Francesco Addeo
Lina Chianese
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Rosalba Mauriello
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Nunziatina Intorcia
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Luigi Moio
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Francesco Addeo
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
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Summary

A new αs2-casein variant was isolated from goat milk by means of covalent chromatography. With gel electrophoresis at alkaline pH, the αs2-casein variant showed a double band with the highest anodic mobility amongst the casein components. The mobility of the isolated αs2-casein variant was similar to that occurring in three individual samples of whole casein. Two dimensional electrophoresis revealed a more pronounced heterogeneity of this protein. Developing the two dimensional plate with polyclonal antibodies obtained against the four main bovine casein fractions, we demonstrated that the variant in question belongs to the αs2-casein family. Since the αs2-casein fractions, immunospecifically developed, spread along two different zones on the gel, it was concluded that a heterozygous αs2-casein form was present in the sample.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 3 , August 1992 , pp. 299 - 305
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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References

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