Skip to main content Accessibility help
×
Home

Metabolomic analysis of alterations in lipid oxidation, carbohydrate and amino acid metabolism in dairy goats caused by exposure to Aflotoxin B1

  • Jianbo Cheng (a1) (a2), Shuai Huang (a1), Caiyun Fan (a1), Nan Zheng (a2) (a3), Yangdong Zhang (a2) (a3), Songli Li (a2) (a3) and Jiaqi Wang (a2) (a3)...

Abstract

The purposes of this study were to investigate the systemic and characteristic metabolites in the serum of dairy goats induced by aflatoxin B1 (AFB1) exposure and to further understand the endogenous metabolic alterations induced by it. A nuclear magnetic resonance (NMR)-based metabonomic approach was used to analyse the metabolic alterations in dairy goats that were induced by low doses of AFB1 (50 µg/kg DM). We found that AFB1 exposure caused significant elevations of glucose, citrate, acetate, acetoacetate, betaine, and glycine yet caused reductions of lactate, ketone bodies (acetate, β-hydroxybutyrate), amino acids (citrulline, leucine/isoleucine, valine, creatine) and cell membrane structures (choline, lipoprotein, N-acetyl glycoproteins) in the serum. These data indicated that AFB1 caused endogenous metabolic changes in various metabolic pathways, including cell membrane-associated metabolism, the tricarboxylic acid cycle, glycolysis, lipids, and amino acid metabolism. These findings provide both a comprehensive insight into the metabolic aspects of AFB1-induced adverse effects on dairy goats and a method for monitoring dairy animals exposed to low doses of AFB1.

Copyright

Corresponding author

*For correspondence; e-mail: jiaqiwang@vip.163.com

Footnotes

Hide All

These authors contributed equally to this work.

Footnotes

References

Hide All
Baldwin, S & Parker, RS 1986 Effects of dietary fat level and aflatoxin B1 treatment on rat hepatic lipid composition. Food and Chemical Toxicology 23 10491055
Bedard, LL & Massey, TE 2006 Aflatoxin B1-induced DNA damage and its repair. Cancer Letters 241 174183
Bennett, JW & Klich, M 2003 Mycotoxins. Clinical Microbiology Reviews 16 497516
Commission, E 2006 Commission regulation (EC) No 1881/2006 of 19 December 2006, setting maximum levels for certain contaminants in foodstuff, pp. 5–24. Official Journal of the European Union
Creppy, EE 2002 Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicology Letters 127 1928
Diao, C, Zhao, L, Guan, M, Zheng, Y, Chen, M, Yang, Y, Lin, L, Chen, W & Gao, H 2014 Systemic and characteristic metabolites in the serum of streptozotocin-induced diabetic rats at different stages as revealed by a (1)H-NMR based metabonomic approach. Molecular Biosystems 10 686693
Dudka, I, Kossowska, B, Senhadri, H, Latajka, R, Hajek, J, Andrzejak, R, Antonowicz-Juchniewicz, J & Gancarz, R 2014 Metabonomic analysis of serum of workers occupationally exposed to arsenic, cadmium and lead for biomarker research: a preliminary study. Environment International 68 7181
Ellinger-Ziegelbauer, H, Stuart, B, Wahle, B, Bomann, W & Ahr, HJ 2004 Characteristic expression profiles induced by genotoxic carcinogens in rat liver. Toxicological Sciences 77 1934
He, J, Zhang, KY, Chen, DW, Ding, XM, Feng, GD & Ao, X 2013 Effects of maize naturally contaminated with aflatoxin B1 on growth performance, blood profiles and hepatic histopathology in ducks. Livestock Science 152 192199
Hopton, RP, Turner, E, Burley, VJ, Turner, PC & Fisher, J 2010 Urine metabolite analysis as a function of deoxynivalenol exposure: an NMR-based metabolomics investigation. Food Additives and Contaminants 27 255261
Hsieh, LL, Hsu, SW, Chen, DS & Santella, RM 1988 Immunological detection of aflatoxin B1-DNA adduca formed in vivo. Cancer Research 48 62316328
IARC (International agency for research on cancer) 2002 Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. In IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, pp. 1556 Ed. World Health Organization: France
Kiessling, KH & Adam, G 1986 Biochemical mechanism of action of mycotoxins. Pure and Applied Chemistry 58 327338
Lu, XY, Hu, B, Shao, L, Tian, Y, Jin, TT, Jin, YC, Ji, S & Fan, XH 2013 Integrated analysis of transcriptomics and metabonomics profiles in aflatoxin B1-induced hepatotoxicity in rat. Food and Chemical Toxicology 55 444455
Mclean, M & Dutton, MF 1995 Cellular interactions and metabolism of aflatoxin: An update. Pharmacology & Therapeutics 65 163192
Mishra, HN & Das, C 2003 A review on biological control and metabolism of aflatoxin. Critical Reviews in Food Science and Nutrition 43 245264
Ortatatli, M & Oğuz, H 2001 Ameliorative effects of dietary clinoptilolite on pathological changes in broiler chickens during aflatoxicosis. Research in Veterinary Science 71 5966
Sabbioni, G & Sepai, O 1998 Determination of human exposure to aflatoxins. In Mycotoxins in Agriculture and Food Safety, pp. 183226 (Eds Sinha, KK & Bhatnagar, D). New York: Marcel Dekker, Inc., Publisher
SAC (Standardization Administration of the People's Republic of China) 2001 Hygienical Standard for Feeds, p. 20. Beijing: Standards Press of China Publisher
SAC (Standardization Administration of the People's Republic of China) 2003 Maximum Levels of Aflatoxin M1 in Milk and Milk Products, p. 1. Beijing: Standards Press of China Publisher
Sieber, M, Wagner, S, Rached, E, Amberg, A, Mally, A & Dekant, W 2009 Metabonomic study of ochratoxin a toxicity in rats after repeated administration: phenotypic anchoring enhances the ability for biomarker discovery. Chemical Research in Toxicology 22 12211231
Smela, ME, Currier, SS, Bailey, EA & Essigmann, JM 2001 The chemistry and biology of aflatoxin B(1): from mutational spectrometry to carcinogenesis. Carcinogenesis 22 535545
Smith, JE & Moss, MO 1985 Mycotoxins. Formation, Analysis and Significance, p. 148. Wiley Publisher
Sundekilde, UK & Poulsen, NA 2013 Nuclear magnetic resonance metabonomics reveals strong association between milk metabolites and somatic cell count in bovine milk. Journal of Dairy Research 96 290299
Tessari, ENC, Kobashigawa, E, Cardoso, ALSP, Ledoux, DR, Rottinghaus, GE & Oliveira, CAF 2010 Effects of aflatoxin B1 and fumonisin B1 on blood biochemical parameters in broilers. Toxins 2 453460
Tian, H, Wang, WY, Zheng, N, Cheng, JB, Li, SL, Zhang, YD & Wang, JQ 2015 Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows. Journal of Proteomics 125 1728
Wan, QF, He, QH, Deng, XB, Hao, FH, Tang, HR & Wang, YL 2016 Systemic metabolic responses of broiler chickens and piglets to acute T-2 toxin intravenous exposure. Journal of Agricultural and Food Chemistry 64 714723
Yarru, LP, Settivari, RS, Antoniou, E, Ledoux, DR & Rottinghaus, GE 2009 Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male broiler chicks. Poultry Science 88 36371
Zerbst-Boroffka, I, Kamaltynow, RM, Harjes, S, Kinne-Saffran, E & Gross, J 2005 TMAO and other organic osmolytes in the muscles of amphipods (crustacea) from shallow and deep water of lake baikal. Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology 142 5864
Zhang, LM, Ye, YF, An, YP, Tian, Y, Wang, YL & Tang, HR 2011 Systems responses of rats to aflatoxin B1 exposure revealed with metabonomic changes in multiple biological matrices. Journal of Proteome Research 10 614623
Zhou, QB, Xie, H, Zhang, L, Stewart, JK, Gu, XX & Ryan, JJ 2006 Cis-Terpenones as an effective chemopreventive agent against aflatoxin B1-induced cytotoxicity and TCDD-induced P450 1A/B activity in HepG2 cells. Chemical Research in Toxicology 19 14151419
Zhuang, ZH, Lin, YN, Yang, C, Yuan, J & Wang, SH 2014 Study on CPS1: The key gene of urea cycle under the stress of aflatoxin B1. Asian Journal of Chemistry 26 33053310

Keywords

Type Description Title
PDF
Supplementary materials

Cheng et al supplementary material
Cheng et al supplementary material 1

 PDF (334 KB)
334 KB

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed