Skip to main content Accessibility help
×
Home

Metabolism of [U-14C]D-fructose by the isolated perfused udder

  • R. Verbeke (a1), Anne-Marie Massart-Leën (a1) and G. Peeters (a1)

Summary

A lactating mammary gland of a sheep and a goat were perfused for several hours in the presence of [U-14C]D-fructose and received adequate quantities of acetate, glucose and amino acids.

In both experiments, there was a small incorporation of 14C in the expired CO2. Smaller radioactivities were measured in milk citric acid, lactose, casein and fat, the activities decreasing in that order. The specific activities of the amino acids from one casein hydrolysate were determined. The highest radioactivities were found in alanine and serine; methionine, glutamic acid and aspartic acid showed a smaller incorporation of 14C.

These results indicate that fructose is metabolized only to a very limited extent by the mammary gland. The relative distribution of 14C observed among the different substances isolated may be explained by a direct splitting of fructose into two C3-fragments, glycolysis and metabolism via the Krebs cycle.

Copyright

References

Hide All
Black, A. L. & Kleiber, M. (1962). In Symposium on use of Radioisotopes in Animal Biology and Medical Sciences, vol. 2, p. 137 (ed. Fried, M.). London and New York: Academic Press.
Carroll, K. K. (1961). J. Lipid Res. 2, 135.
Hardwick, D. C. & Linzell, J. L. (1960). J. Physiol., Lond. 154, 547.
Hers, H. G. (1955). J. biol. Chem. 214, 373.
Hers, H. G. & Kusaka, T. (1953). Biochim. biophys. Acta 11, 427.
Heyndrick, G. V. & Peeters, G. J. (1960). Biochem. J. 75, 1.
Lascelles, A. K., Hardwick, D. C., Linzell, J. L. & Mepham, T. B. (1964). Biochem. J. 92, 36.
Luick, J. R., Kleiber, M. & Lucas, J. M. (1959). Am. J. Physiol. 197, 677.
Luick, J. R., Kleiber, M., Lucas, J. M. & Rogers, T. A. (1957). Am. J. Physiol. 191, 90.
Meister, A. (1965). In Biochemistry of the Amino Acids, Vol. II. New York, London: Academic Press.
Muntz, J. A. (1968). J. biol. Chem. 243, 2788.
Somogyi, M. (1945). J. biol. Chem. 160, 61.
Verbeke, R., Feteanu, A. & Peeters, G. (1967). Archs int. Physiol. Biochim. 75, 675.
Verbeke, R., Lauryssens, M., Peeters, G. & James, A. T. (1959). Biochem. J. 73, 24.
Verbeke, R., Peeters, G., Massart-Leën, A. M. & Cocquyt, G. (1968). Biochem. J. 106, 719.
Verbeke, R., Simeonov, S. & Peeters, G. (1967). Archs int. Physiol. Biochim. 75, 658.
Walborg, E. F., Christensson, L. & Gardell, S. (1965). Analyt. Biochem. 13, 177.
Wood, H. G., Joffe, S., Gillespie, R., Hansen, R. G. & Hardenbrook, H. (1958). J. biol. Chem. 233, 1264.

Metabolism of [U-14C]D-fructose by the isolated perfused udder

  • R. Verbeke (a1), Anne-Marie Massart-Leën (a1) and G. Peeters (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed