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Locus BoLA-DRB3 is just an ordinary site of the polygene when explaining genetic variance of somatic cell count and milk yield

  • Jolanta Oprzadek (a1), Grazyna Sender (a1), Adrianna Pawlik (a1) and Marek Lukaszewicz (a1)


The study aimed at clarifying the problem of the hitherto contradictory results regarding usefulness of BoLA-DRB3 locus as a marker in selection against mastitis and for milk yield. Treating the BoLA-DRB3 locus effect as random was proposed in place of considering it fixed. Somatic cell counts and milk yields recorded monthly on a test day (22 424) of 619 Polish Holstein cows genotyped for BoLA-DRB3 were analysed with an animal model including a random effect for genotype at this locus. The BoLA-DRB3 alleles were defined as restriction patterns obtained with three endonucleases. Two alternative BoLA-DRB3 additive genotype (co)variance structures were constructed for 161 genotypes recorded. One was based on the allelic similarity of the genotypes resulting in element values of 0 (no common allele), 0·5 (one allele in common), and 1 (diagonal). The other considered restriction site similarity (up to 3 in 1 allele) giving element values of 0 (no common restriction sites) and then increasingly in steps of 1/6 up to 6/6 (diagonal), where the numerator represents the number of common sites between genotypes. The DRB3 variance component for the natural logarithm of somatic cell count did not exceed 0·006 of the polygenic additive component or 0·003 for milk yield. Hence, unless we fail to detect the causative site or to properly define traits being the projection of a site, the effect of the genotype at the BoLA-DRB3 locus does not explain variation in somatic cell count and milk yield at a degree expected of a genetic marker.


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Andersson, L, Böhme, J, Peterson, PA & Rask, L 1986 Genomic hybridization of bovine class II major histocompatibility genes: 2. Polymorphism of DR genes and linkage disequilibrium in the DQ DR region. Animal Genetics 17 295304
Baltian, LR, Ripoli, MV, Sanfilippo, S, Takeshima, S-N, Aida, Y & Giovambattista, G 2012 Association between BoLA-DRB3 and somatic cell count in Holstein cattle from Argentina. Molecular Biology Reports 39 72157220
Cole, JB, VanRaden, PM, O'Connell, JR, Van Tassell, CP, Sonstegard, TS, Schnabel, RD, Taylor, JF & Wiggans, GR 2009 Distribution and location of genetic effects for dairy traits. Journal of Dairy Science 92 29312946
Duangjinda, M, Buayai, D, Pattarajinda, V, Phasuk, Y, Katawatin, S, Vongpralub, T & Chaiyotvittayakul, A 2009 Detection of bovine leukocyte antigen DRB3 alleles as candidate markers for clinical mastitis resistance in Holstein × Zebu. Journal of Animal Science 87 469476
Juliarena, MA, Poli, M, Sala, L, Ceriani, C, Gutierrez, S, Dolcini, G, Rodriguez, EM, Marińo, B, Rodriguez-Dubra, C & Esteban, EN 2008 Association of BLV infection profiles with alleles of the BoLA-DRB3·2 gene. Animal Genetics 39 432438
Misztal, I 2006 Challenges of application of marker assisted selection – a review. Animal Science Papers and Reports 24 510
Misztal, I 2008 Reliable computing in estimation of variance components. Journal of Animal Breeding and Genetics 125 363370
Misztal, I, Tsuruta, S, Strabel, T, Auvray, B, Druet, T & Lee, DH 2002 BLUPF90 and related programs (BGF90). 7th World Congress on Genetics Applied to Livestock Production, August 19–23, 2002, Montpellier, France, Communication N°28–07 – available at (accessed 12 Jul 2015)
Oprządek, J, Urtnowski, P, Sender, G, Pawlik, A & Łukaszewicz, M 2012 Frequency of BoLA-DRB3 alleles in Polish Holstein-Friesian cattle. Animal Science Papers and Reports 30 91101
Rupp, R, Hernandez, A, & Mallard, BA 2007 Association of bovine leukocyte antigen (BOLA) DRB3·2 with immune response, mastitis, and production and typed traits in Canadian Holstein. Journal of Dairy Science 90 10291038
Sharif, S, Mallard, BA, Wilkie, BN, Sargeant, JM, Scott, HM, Dekkers, JC & Leslie, KE 1998 Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) with production traits in Canadian dairy cattle. Animal Genetics 30 157160
Solbu, H & Lie, O 1990 Selection for disease resistance in dairy cattle. Proc 2nd World Congress on Genetics Applied to Livestock Production 16 445448
Van Eijk, MJT, Stewart-Haynes, JA & Lewin, HA 1992 Extensive polymorphism of the BoLA DRB3 gene distinguished by PCR-RFLP. Animal Genetics 23 483496
Yoshida, T, Mukoyama, H, Furuta, H, Kondo, Y, Takeshima, S-N, Aida, Y, Kosugiyama, M & Tomogane, H 2009 Association of BoLA-DRB3 alleles identified by sequence-based typing method with mastitis pathogens in Japanese Holstein cows. Animal Science Journal 80 498509
Yoshida, T, Furuta, H, Kondo, Y & Mukoyama, H 2012 Association of BoLA-DRB3 alleles with mastitis resistance and susceptibility in Japanese Holstein cows. Animal Science Journal 83 359366



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