Skip to main content Accessibility help

Lipoproteolytic capacity and potential of Pseudomonas spp. isolated from cold raw milk

  • Bruna de Moura Aguiar (a1), Rosana de Longhi (a1), Regina Celia Poli-Frederico (a2), Rafael Fagnani (a1) and Elsa H W de Santana (a1)...


The objective of the work described in this research communication was to determine the lipoproteolytic capacity and potential of Pseudomonas spp. from the microbiota of refrigerated raw milk. The influence of temperature and bacterial population on these activities was also evaluated. Pseudomonas spp. (PS) counts (30 °C/48 h) were confirmed by PCR. Proteolytic (10% milk agar) and lipolytic capacities (PLC) (tributyrin agar) were evaluated (21 °C/72 h). Proteolytic (PP) and lipolytic potential (LP) were assessed by measuring the diameter of the halos and were categorized as low or high. A total of 91.3% PS possessed PLC. The PP of 64.16% isolates was high and was frequently observed in PS from milk samples with higher counts and lower temperatures. The LP of 70.52% isolates was low, and higher LP was associated with low microbiological counts and temperatures. Genetic studies evaluating Pseudomonas spp. strains in the milking environment and investigating the origin of these isolates could be useful to improve the quality and shelf life of dairy products.


Corresponding author

Author for correspondence: Elsa HW de Santana, Email:


Hide All
Al–Rodhan, AM and Nasear, HA (2016) PCR-Based detection of Pseudomonas fluorescens in cows and buffalos raw milk. Brazilian Journal of Veterinary Research 15, 194208.
Chen, L, Daniel, RM and Coolbear, T (2003) Detection and impact of protease and lipase activities in milk and milk powders. International Dairy Journal 13, 255275.
Decimo, M, Morandi, S, Silvetti, T and Brasca, M (2014) Characterization of Gram negative psychrotrophic bacteria isolated from Italian bulk tank milk. Journal of Food Science 79, 20812090.
Deeth, HC and Fitz-Gerald, CH (2005) Lipolytic enzymes and hydrolytic rancidity. In Fox, PF and MCsweeney, PLH (eds), Advanced Dairy Chemistry: Lipids, 3th Edn.New York: Springer, pp. 481556.
Ercolini, D, Russo, F, Ferrocino, I and Villani, F (2009) Molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk. Food Microbiology 26, 228231.
Frank, JF, Christen, GL and Bullerman, LB (1992) Tests for groups of microorganisms. In Marshall, RT (ed.), Standard Methods for the Examination of Dairy Products, 6th Edn.Washington, USA: American Public Health Association, pp. 275276.
Meng, L, Zhang, Y, Liu, H, Zhao, S, Wang, J and Zheng, N (2017) Characterization of Pseudomonas spp. and associated proteolytic properties in raw milk stored at low temperatures. Frontiers Microbiology 8, 17.
Oliveira, GB, Favarin, L, Luchese, RH and McIntosh, D (2015) Psychrotrophic bacteria in milk: how much do we really know? Brazilian Journal of Microbiology 46, 313321.
Oliveria, JS and Parmelle, CE (1976) Rapid enumeration of psychrotrophic bacteria in raw and pasteurized milk. Journal Milk of Food and Technology 39, 269272.
Samaržija, D, Zamberlin, Š and Pogačić, T (2012) Psychrotrophic bacteria and milk and dairy products quality. Mljekarstvo 62, 7795.
Spilker, T, Coenye, T, Vandamme, P and LiPuma, JJ (2004) PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. Journal of Clinical Microbiology 42, 20742079.
Xin, L, Meng, Z, Zhang, L, Cui, Y, Han, XE and Yi, H (2017) The diversity and proteolytic properties of psychrotrophic bacteria in raw cows’ milk from North China. International Dairy Journal 66, 3441.
Woods, RG, Burger, M, Beven, CA and Behringer, IR (2001) The aprX–lipA operon of Pseudomonas fluorescens B52: a molecular analysis of metalloprotease and lipase production. Microbiology (Reading, England) 147, 345354.


Related content

Powered by UNSILO
Type Description Title
Supplementary Material

Moura Aguiar et al. supplementary material
Moura Aguiar et al. supplementary material

 PDF (291 KB)
291 KB

Lipoproteolytic capacity and potential of Pseudomonas spp. isolated from cold raw milk

  • Bruna de Moura Aguiar (a1), Rosana de Longhi (a1), Regina Celia Poli-Frederico (a2), Rafael Fagnani (a1) and Elsa H W de Santana (a1)...


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.