Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-18T06:01:45.717Z Has data issue: false hasContentIssue false
  • English
  • Français

Lack of effect of epidermal growth factor treatment in late-pregnant ewes on subsequent lactation

Published online by Cambridge University Press:  01 June 2009

Christine B. Gow ,
Debbi J. Singleton ,
Mervyn J. Silvapulle  and
G. Philip M. Moore
Christine B. Gow
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
Debbi J. Singleton
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
Mervyn J. Silvapulle
Affiliation:
School of Agriculture, La Trobe University, Bundoora, Victoria 3083, Australia
G. Philip M. Moore
Affiliation:
CSIRO, Division of Animal Production, PO Box 239, Blacktown, New South Wales 2148, Australia
Get access Rights & Permissions [Opens in a new window]

Summary

Twin-bearing ewes were treated with epidermal growth factor (EGF) to determine its effect on mammogenesis and resultant milk production and composition. The EGF was infused intravenously at a dose rate of O5 mg/d in 300 ml saline between days 117 and 139 of gestation; control animals received placebo infusions of saline. All animals then received continuous infusions of 300 ml/d saline on days 139–144. Following parturition 1–5 d later, ewes were milked by hand for 10 d and thereafter were machine-milked until day 16 of lactation. At this level of treatment, EGF was not detected in the circulation during infusion and feed intake was not affected. All ewes gave birth to healthy twin lambs. There were no effects of EGF on birth weights of lambs, live weights of ewes or lengths of gestation. An EGF immunoreactive material was detected in the mammary secretions of control ewes at a mean concentration of 2 μg/l on day 1 of lactation. Two ewes had detectable levels on day 2, but none was found in the milk thereafter. In the EGF-infused group, concentrations of EGF in colostrum were ñ 10 times higher than in the control ewes on day 1 of lactation and EGF was detected in mammary secretions on day 2 but not in subsequent milk samples. A range of 0·3–0·5% of the EGF infused appeared in mammary secretions over the first 2 d of lactation. No other differences were observed for colostrum composition, subsequent milk yield or composition between the two groups of ewes indicating that mammary gland development and function were unaffected. The levels of EGF observed in the mammary secretions of treated and control ewes indicate that the mammary glands accumulate and store EGF in the pre partum period.

Type
Original articles
Information
Journal of Dairy Research , Volume 58 , Issue 1 , February 1991 , pp. 1 - 11
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beardmore, J. M. & Richards, R. C. 1983 Concentrations of epidermal growth factor in mouse milk throughout lactation. Journal of Endocrinology 96 287–292CrossRefGoogle ScholarPubMed
Blakeley, D. M., Brown, K. D. & Fleet, I. R. 1982 Transfer of epidermal growth factor from blood to milk in lactating goats. Journal of Physiology 326 57P–58PGoogle Scholar
Brown, K. D. & Blakeley, D. M. 1983 a Cell growth-promoting activity in mammary secretions of the goat, cow and sheep. British Veterinary Journal 139 68–78CrossRefGoogle ScholarPubMed
Brown, K. D. & Blakeley, D. M. 1983 b Inhibition of the binding of 125I-labelled epidermal growth factor to mouse cells by a mitogen in goat mammary secretions. Biochemical Journal 212 465–472CrossRefGoogle ScholarPubMed
Brown, K. D. & Blakeley, D. M. 1984 Partial purification and characterization of a growth factor present in goat's colostrum: similarities with platelet-derived growth factor. Biochemical Journal 219 609–617CrossRefGoogle ScholarPubMed
Brown, K. D., Blakeley, D. M., Fleet, I. R., Hamon, M. & Heap, R. B. 1986 Kinetics of transfer of 125I-labelled epidermal growth factor from blood into mammary secretions of goats. Journal of Endocrinology 109 325–332CrossRefGoogle ScholarPubMed
Burgess, A. W. 1989 Epidermal growth factor and transforming growth factor α. British Medical Bulletin 45 401–424CrossRefGoogle ScholarPubMed
Byyny, R. L., Orth, D. N., Cohen, S. & Doynk, E. S. 1974 Epidermal growth factor: effects of androgens and adrenergic agents. Endocrinology 95 776–782CrossRefGoogle ScholarPubMed
Carpenter, G. 1978 The regulation of cell proliferation: advances in the biology and mechanism of action of epidermal growth factor. Journal of Investigative Dermatology 71 283–286CrossRefGoogle ScholarPubMed
Carpenter, G. & Cohen, S. 1976 125I-labeled human epidermal growth factor. Binding, internalization and degradation in human fibroblasta. Journal of Cellular Biology 71 159–171CrossRefGoogle Scholar
Carter, N. B., Fawcett, A. A., Hales, J. R. S., Moore, G. P. M. & Panaretto, B. A. 1988 Circulatory effects of a depilatory dose of mouse epidermal growth factor in sheep. Journal of Physiology 403 27–39CrossRefGoogle ScholarPubMed
Cohen, S. 1962 Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. Journal of Biological Chemistry 237 1555–1562CrossRefGoogle ScholarPubMed
Cohen, S. 1965 The stimulation of epidermal proliferation by a specific protein (EGF). Developmental Biology 12 394–407CrossRefGoogle ScholarPubMed
Coleman, S., Silberstein, G. B. & Daniel, C. W. 1988 Ductal morphogenesis in the mouse mammary gland: evidence supporting a role for epidermal growth factor. Developmental Biology 127 304–315CrossRefGoogle ScholarPubMed
Collier, R. J. & McGrath, M. F. 1988 Effect of human epidermal growth factor (hEGF) on bovine mammary development in vivo. Journal of Dairy Science 71 (Suppl) 228Google Scholar
Corcoran, J. N., Waters, M. J., Eastman, C. J. & Jorgensen, G. 1986 Epidermal growth factor: effect on circulating thyroid hormone levels in sheep. Endocrinology 119 214–217CrossRefGoogle ScholarPubMed
Cowie, A. J., Forsyth, I. A. & Hart, I. C. 1980 Hormonal Control of Lactation. Berlin: Springer-Verlag. (Monographs on Endocrinology, Vol. 15)CrossRefGoogle ScholarPubMed
Ethier, S. P., Kudla, A. & Cundiff, K. C. 1987 Influence of hormone and growth factor interactions on the proliferative potential of normal rat mammary epithelial cells in vitro. Journal of Cellular Physiology 132 161–167CrossRefGoogle ScholarPubMed
Forsyth, I. A. 1989 Growth factors in mammary gland function. Journal of Reproduction and Fertility 85 759–770CrossRefGoogle ScholarPubMed
Freemark, M. & Comer, M. 1987 Epidermal growth factor (EGF)-like transforming growth factor (TGF) activity and EGF receptors in ovine fetal tissues: possible role for TGF in ovine fetal development. Pediatric Research 22 609–615CrossRefGoogle ScholarPubMed
Gallo-Payet, N., Pothier, P. & Hugon, J. S. 1987 Ontogeny of EGF receptors during the postnatal development of mouse small intestine. Journal of Pediatric Gastroenterology and Nutrition 6 114–120Google ScholarPubMed
Gospodarowicz, D. 1981 Epidermal and nerve growth factors in mammalian development. Annual Review of Physiology 43 251–263CrossRefGoogle ScholarPubMed
Gresik, E. W., Van Der Noen, H. & Barka, T. 1984 Transport of 125I-EGF into milk and effect of sialoadenectomy on milk EGF in mice. American Journal of Physiology 247 E349–E354Google ScholarPubMed
Grueters, A., Alm, J., Lakshmanan, J. & Fisher, D. A. 1985 Epidermal growth factor in mouse milk during early lactation: lack of dependency on submandibular glands. Pediatric Research 19 853–856CrossRefGoogle ScholarPubMed
Imagawa, W., Tomooka, Y., Hamamoto, S. & Nandi, S. 1985 Stimulation of mammary epithelial cell growth in vitro: interaction of epidermal growth factor and mammogenic hormones. Endocrinology 116 1514–1524CrossRefGoogle ScholarPubMed
Klagsbrun, M. 1978 Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts. Proceedings of the National Academy of Sciences, USA 75 5057–5061CrossRefGoogle ScholarPubMed
Klagsbrun, M. 1980 Bovine colostrum supports the serum-free proliferation of epithelial cells but not of fibroblasts in long-term culture. Journal of Cell Biology 84 808–814CrossRefGoogle Scholar
Liu, S. C., Sanfilippo, B., Perroteau, I., Derynck, R., Salomon, D. S. & Kidwell, W. R. 1987 Expression of transforming growth factor α (TGFα) in differentiated rat mammary tumors: estrogen induction of TGFα production. Molecular Endocrinology 1 683–692CrossRefGoogle ScholarPubMed
McGrath, M. F. & Collier, R. J. 1988 Effect of epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) on ruminant mammary development. Journal of Dairy Science 71 (Suppl.) 229.Google Scholar
Mellor, D. J. & Murray, L. 1985 Effects of maternal nutrition on udder development during late pregnancy and on colostrum production in Scottish Blackface ewes with twin lambs. Research in Veterinary Science 39 230–234CrossRefGoogle ScholarPubMed
Moore, G. P. M., Panaretto, B. A. & Robertson, D. 1982 Inhibition of wool growth in Merino sheep following adminstration of mouse epidermal growth factor and a derivative. Australian Journal of Biological Sciences 35 163–172CrossRefGoogle Scholar
Moore, G. P. M., Panaretto, B. A. & Wallace, A. L. C. 1984 Treatment of ewes at different stages of pregnancy with epidermal growth factor: effects on wool growth and plasma concentrations of growth hormone, prolactin, placental lactogen and thyroxine and on foetal development. Acta Endocrinohgica 105 558–566.Google ScholarPubMed
Moore, G. P. M., Wilkinson, M., Panaretto, B. A., Delbridge, L. W. & Posen, S. 1986 a Epidermal growth factor causes hypocalcemia in sheep. Endocrinology 118 1525–1529CrossRefGoogle ScholarPubMed
Moore, G. P. M., Wynn, P. C., Djura, P. & Van Dooren, P. H. 1986 b Regulation of epidermal cell proliferation by epidermal growth factor (EGF) and related peptides. Acta Biologica Hungarica (Suppl.) 44Google Scholar
Moore, N. W. & Holst, P. J. 1967 The evaluation of progesterone- and SC-9880-impregnated intravaginal sponges used with PMS for the induction of breeding in the anoestrous crossbred ewe. In The Control of the Ovarian Cycle in the Sheep pp. 133–143 (Ed. Robinson, T. J.). Sydney: Sydney University PressGoogle Scholar
Okamoto, S. & Oka, T. 1984 Evidence for physiological function of epidermal growth factor: pregestational sialoadenectomy of mice decreases milk production and increases offspring mortality during lactation period. Proceedings of the National Academy of Sciences, USA 81 6059–6063CrossRefGoogle ScholarPubMed
Panaretto, B. A., Moore, G. P. M. & Robertson, D. M. 1982 Plasma concentrations and urinary excretion of mouse epidermal growth factor associated with the inhibition of food consumption and of wool growth in Merino wethers. Journal of Endocrinology 94 191–202CrossRefGoogle ScholarPubMed
Popliker, M., Shatz, A., Avivi, A., Ullrich, A., Schlessinger, J. & Webb, C. G. 1987 Onset of endogenous synthesis of epidermal growth factor in neonatal mice. Developmental Biology 119 38–44CrossRefGoogle ScholarPubMed
Read, L. C. 1988 Milk growth factors. In Fetal and Neonatal Growth Vol. 5, pp. 131–152 (Ed. Cockburn, F.) Brisbane: John Wiley & SonsGoogle Scholar
Read, L. C., Francis, G. L., Wallace, J. C. & Ballard, F. J. 1985 Growth factor concentrations and growth-promoting activity in human milk following premature birth. Journal of Developmental Physiology 7 135–145Google ScholarPubMed
Read, L. C., Upton, F. M., Francis, G. L., Wallace, J. C., Dahlenberg, G. W. & Ballard, F. J. 1984 Changes in the growth-promoting activity of human milk during lactation. Pediatric Research 18 133–139CrossRefGoogle ScholarPubMed
Savage, C. R. & Cohen, S. 1972 Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. Journal of Biological Chemistry 247 7609–7611CrossRefGoogle Scholar
Shaw, G., Jorgensen, G. I., Tweedale, R., Tennison, M. & Waters, M. J. 1985 Effect of epidermal growth factor on reproductive function of ewes. Journal of Endocrinology 107 429–436CrossRefGoogle ScholarPubMed
Snedecor, G. W. & Cochran, W. G. 1976 Statistical-Methods, 6th ednAmes, IA: Towa State University PressGoogle Scholar
Sundell, H., Serenius, F. S., Barthe, P., Friedman, Z., Kanarek, K. S., Escasbedo, M. B., Orth, D. N. & Stahliman, M. T. 1975 The effect of EGF on fetal lamb lung maturation. Pediatric Research 9 371Google Scholar
Taketani, Y. & Oka, T. 1983 a Epidermal growth factor stimulates cell proliferation and inhibits functional differentiation of mouse mammary epithelial cells in culture. Endocrinology 113 871–877CrossRefGoogle ScholarPubMed
Taketani, Y. & Oka, T. 1983 b Biological action of epidermal growth factor and its functional receptors in normal mammary epithelial cells. Proceedings of the National Academy of Sciences, USA 80 2647–2650CrossRefGoogle ScholarPubMed
Taketani, Y. & Oka, T. 1983 c Possible physiological role of epidermal growth factor in the development of the mouse mammary gland during pregnancy. FEBS Letters 152 256–260CrossRefGoogle ScholarPubMed
Thompson, J. F. 1986 Evidence for specific epidermal growth factor (EGF) receptors on fetal, suckling and adult rat intestinal microvillus membranes (MVM). Gastroenterology 90 1664aGoogle Scholar
Thornburg, W., Matrisian, L., Magun, B. & Koldovsky, O. 1984 Gastrointestinal absorption of epidermal growth factor in suckling rats. American Journal of Physiology 246 G80–G85Google ScholarPubMed
Tonelli, Q. J. & Sorof, S. 1980 Epidermal growth factor requirement for development of cultured mammary gland. Nature 285 250–252CrossRefGoogle ScholarPubMed
Vonderhaar, B. K. 1987 Local effects of EGF, α-TGF and EGF-like growth factors on lobuloalveolar development of the mouse mammary gland in vivo. Journal of Cellular Physiology 132 581–584CrossRefGoogle ScholarPubMed
Winder, S. J., Turvey, A. & Forsyth, I. A. 1989 Stimulation of DNA synthesis in cultures of ovine mammary epithelial cells by insulin and insulin-like growth factors. Journal of Endocrinology 123 319–326CrossRefGoogle ScholarPubMed
Wynn, P. C., Maddocks, I. G., Moore, G. P. M., Panaretto, B. A., Djura, P., Ward, W. G., Fleck, E. & Chapman, R. E. 1989 Characterization and localization of receptors for epidermal growth factor in ovine skin. Journal of Endocrinology 121 81–90CrossRefGoogle ScholarPubMed