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Influence of the lactoperoxidase system on susceptibility of the udder to Streptococcus uberis infection

  • Valerie M. E. Marshall (a1), Wendy M. Cole (a1) and A. John Bramley (a1)


Lactoperoxidase (LP), thiocyanate (SCN-), pH and somatic cell counts (SCC) were measured in mammary secretions from 20 cows collected 14 d before drying-off, 7 and 21 d after drying-off, and 3–18 d postcalving. The inhibitory activity of the secretions on Streptococcus uberis was determined and the susceptibility of the udder to infection by this organism was tested by intramammary infusion of 250 colony forming units at the above stages. LP, SCN-, pH and SCO increased during involution and fell postcalving. The secretions collected before drying-off, 7 d after drying-off and postcalving inhibited growth of Str. uberis.; those collected 21 d after drying-off did not. Inhibitory activity in pre-drying-off secretions was destroyed by heating and restored by addition of LP, glucose and glucose oxidase, but addition of these substances to secretion 21 d after drying-off did not provide a full inhibitory system. The growth of Str. uberis in the secretions was correlated with intramammary susceptibility, since challenges with Str. uberis at 14 d before drying-off, at 7 and 21 d after drying-off and postcalving led to 43·8, 25·0, 81·3 and 37·5% of quarters becoming infected. It is suggested that the LP/SCN-/H2O2 system plays a role in protecting the lactating mammary gland from infection with Str. uberis but becomes ineffective as involution progresses.



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Anon. 1981 Laboratory methods for use in mastitis work. International Dairy Federation Bulletin. Document 132
Aune, T. M. & Thomas, E. L. 1977 Accumulation of hypothiocyanite ion during peroxidase-catalyzed oxidation of thiocyanate ion. European Journal of Biochemistry 80 209214
Aune, T. M. & Thomas, E. L. 1978 Oxidation of protein sulfhydryls by products of peroxidase-catalyzed oxidation of thiocyanate ion. Biochemistry 17 10051010
Björck, L. & Claesson, O. 1980 Correlation between concentration of hypothiocyanate and antibacterial effect of the lactoperoxidase system against Escherichia coli. Journal of Dairy Science 63 919922
Björck, L., Claesson, O. & Schulthes, W. 1979 The lactoperoxidase/thiocyanate/hydrogen peroxide system as a temporary preservative for raw milk in developing countries. Milchwiasenschaft 34 726729
Brown, R. W. & Miokelson, M. N. 1979 Lactoperoxidase, thiocyanate and free cystine in bovine mammary secretions in early dry period and at the start of lactation and their effect on Streptococcus agalaetiae growth. American Journal of Veterinary Research 40 250255
Cousins, C. L., Higgs, T. M., Jackson, E. R., Neave, F. K. & Dodd, F. H. 1980 Susceptibility of the bovine udder to bacterial infection in the dry period. Journal of Dairy Research 47 1118
Cosby, E. L. & Sumner, J. B. 1945 Rhodanese. Archives of Biochemistry 7 457460
Dodd, F. N. & Griffin, T. K. 1975 The role of antibiotics treatment at drying off in the control of mastitis. International Dairy Federation Bulletin Document 85 pp. 282302
Garvie, E. I. & Bramley, A. J. 1979 Streptococcus uberis: an approach to its classification. Journal of Applied Bacteriology 46 295304
Homan-Müller, J. W. T., Weenino, R. S. & Roos, D. 1975 Production of hydrogen peroxide by phagocytizing human granulocytes. Journal of Laboratory and Clinical Medicine 85 198207
Hoooendoorn, H., Piessens, J. P., Scholtes, W. & Stoddard, L. A. 1977 Hypothiocyanite ion; the inhibitor formed by the system lactoperoxidase-thiocyanate-hydrogen peroxide. Caries Research 11 7784
Jensen, D. L. & Eberhart, R. J. 1981 Total and differential cell counts in secretions of the nonlactating bovine mammary gland. American Journal of Veterinary Research 42 743747.
Kobayashi, M., Tanaka, T. & Usui, T. 1982 Inactivation of lysosomal enzymes by the respiratory burst of polymorphonuclear leukocytes. Journal of Laboratory and Clinical Medicine 100 896907
Kruze-Virtonich, J. D. 1983 The aetiology of bovine mastitis caused by Streptococcus uberis. Ph.D. thesis, University of Reading, UK.
Marshall, V. M. E. & Reiter, B. 1980 Comparison of the antibacterial activity of the hypothiocyanite anion towards Streptococcus lactis and Escherichia coli. Journal of General Microbiology 120 513516
McDonald, J. S. & Anderson, A. J. 1981 Experimental infection of bovine mammary glands with Streptococcus uberis during the nonlactating period. American Journal of Veterinary Research 42 465467
Neave, F. K., Dodd, F. H. & Henriques, E. 1950 Udder infections in the ‘dry period’. I. Journal of Dairy Research 17 3749
Oram, J. D. & Reiter, B. 1966 The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. The effect of the inhibitory system on susceptible and resistant strains of group N streptococci. Biochemical Journal 100 373381
Pettipher, G. L. & Rodrioues, U. M. 1981 Rapid membrane filtration epifluorescent microscopic technique for the direct enumeration of somatic cells in fresh and formalin-preserved milk. Journal of Dairy Research 48 239246
Reiter, B. & Bramley, A. J. 1975 Defence mechanisms of the bovine udder and their relevance to mastitis control. International Dairy Federation Bulletin Document 85 pp. 210222
Reiter, B., Marshall, V. M., BjöRCK, L. & Rosen, C. G. 1976 Nonspecific bactericidal activity of the lactoperoxidase/thiocyanate/hydrogen peroxide system of milk against Escherichia coli and some Gram-negative pathogens. Infection and Immunity 13 800807
Reiter, B., Marshall, V. M. & Philips, S. M. 1980 The antibiotic activity of the lactoperoxidase- thiocyanate-hydrogen peroxide system in the calf abomasum. Research in Veterinary Science 28 116122
Reiter, B., Pickering, A., Oram, J. D. & Pope, G. S. 1963 Peroxidase-thiocyanate inhibition of streptococci in raw milk. Journal of General Microbiology 33 xii
Reiter, B., Sharpe, M. E. & Higgs, T. M. 1970 Experimental infection of the non-lactating bovine udder with Staphylococcus aureus and Streptococcus uberis. Research in Veterinary Science 11 1826
Roouinsky, M. 1972 [Experimental mammary infection in the cow at drying-off or during the dry period.] Annates de Recherches Veterinaires 3 493497
Roouinsky, M. 1977 Comparison of Streptococcus uberis and S. infrequens. Pathogenicity for cow udders. Annates de Recherches Vétérinaires 8 153157
Schanbacher, F. L. & Smith, K. L. 1975 Formation and role of unusual whey proteins and enzymes: relation to mammary function. Journal of Dairy Science 58 10481062
Shindler, J. S., Childs, R. E. & Bardsley, W. G. 1976 Peroxidase from human cervical mucus. The isolation and characterisation. European Journal of Biochemistry 65 325331

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Influence of the lactoperoxidase system on susceptibility of the udder to Streptococcus uberis infection

  • Valerie M. E. Marshall (a1), Wendy M. Cole (a1) and A. John Bramley (a1)


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