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Influence of the emulsion droplet type on the rheological characteristics and microstructure of rennet gels from reconstituted milk

Published online by Cambridge University Press:  12 June 2009

Zafir Gaygadzhiev
Affiliation:
Department of Food Science, University of Guelph, Ontario, Canada, N1G 2W1
Arthur Hill
Affiliation:
Department of Food Science, University of Guelph, Ontario, Canada, N1G 2W1
Milena Corredig*
Affiliation:
Department of Food Science, University of Guelph, Ontario, Canada, N1G 2W1
*
*For correspondence; e-mail: mcorredi@uoguelph.ca

Abstract

Rheological and microstructural properties of rennet-induced milk gels containing different fat globules were studied. Recombined milks were prepared by mixing reconstituted low-heat skim milk powder and anhydrous milk fat emulsified with reconstituted skim milk powder (SMP), sodium caseinate (NaCas), whey protein isolate (WPI) or Tween 20. Final elastic modulus of the rennet gels containing WPI- or Tween 20-stabilized fat globules showed significantly lower values compared with those prepared with SMP-emulsified fat globules. SMP-stabilized fat globules interacted with the continuous casein network reinforcing the gel structure. Confocal micrographs supported the rheological data revealing that gels containing SMP-stabilized fat globules formed a tighter network relative to other treatments. Microscopy images also showed some degree of droplet flocculation in the case of gels containing WPI- or Tween 20-stabilized fat globules, and this was most likely the cause of the increase of elastic modulus of these systems. Contrary to reports for acid-induced casein gels, NaCas-stabilized fat globules hindered the formation of rennet gels. These results illustrate that rennet gel structure is affected by droplet-droplet and droplet-casein interactions, which in turn are determined by the composition of the oil-water interface as well as the ionic equilibrium in the reconstituted milk gels.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

Bringe, NA & Kinsella, JE 1986 Influence of calcium-chloride on the chymosin-initiated coagulation of casein micelles. Journal of Dairy Research 53 371379CrossRefGoogle Scholar
Cho, YH, Lucey, JA & Singh, H 1999 Rheological properties of acid milk gels as affected by the nature of the fat globule surface material and the heat treatment of milk. International Dairy Journal 9 537545CrossRefGoogle Scholar
Dalgleish, DG 1992 The enzymatic coagulation of milk. In Advanced dairy chemistry, Vol. 1 Proteins, pp. 579619 (Ed Fox, PF ). London: Elsevier Applied Science PublishersGoogle Scholar
Dalgleish, DG 2006 Food emulsions – their structures and structure-forming properties. Food Hydrocolloids 20 415422CrossRefGoogle Scholar
Dickinson, E 2006 Structure formation in casein-based gels, foams and emulsions. Colloids and Surfaces A 288 3–11CrossRefGoogle Scholar
Euston, SR & Hirst, RL 1999 Comparison of concentration-dependent emulsifying properties of protein products containing aggregated and non-aggregated milk protein International Dairy Journal 9 693701CrossRefGoogle Scholar
Fang, Y & Dalgleish, DG 1993 Dimensions of the adsorbed layers in oil-in-water emulsions stabilized by caseins. Journal of Colloid and Interface Science 156 329334CrossRefGoogle Scholar
Guinee, TP, Gorry, CB, O'Callaghan, DJ, O'Kennedy, BT, O'Brien, N & Fenelon, MA 1997 The effect of composition and some processing treatments on the rennet coagulation properties of milk. International Journal of Dairy Technology 50 99–106CrossRefGoogle Scholar
Gaygadzhiev, Z, Alexander, M & Corredig, M 2009 Sodium caseinate-stabilized fat globules inhibition of the rennet-induced gelation of casein micelles studied by diffusing wave spectroscopy. Food Hydrocolloids 23 11341138CrossRefGoogle Scholar
Lucey, JA 2002 Formation and physical properties of milk protein gels. Journal of Dairy Science 85 281294CrossRefGoogle ScholarPubMed
Michalski, MC, Cariou, FM & Garnier, C 2002a Native vs. damaged milk fat globules: membrane properties affect the viscoelasticity of milk gels. Journal of Dairy Science 85 24512461CrossRefGoogle ScholarPubMed
Michalski, MC, Michel, F, Sainmont, D & Briard, V 2002b Apparent zeta-potential of homogenized milk fat globule as a tool to assess mechanical damages to the milk fat globule membrane. Colloid Surface B: Biointerfaces 23 2330CrossRefGoogle Scholar
Mulvihill, DM & Murphy, PC 1991 Surface active and emulsifying properties of caseins/caseinates as influenced by state of aggregation. International Dairy Journal 1 1337CrossRefGoogle Scholar
Sala, G, Van Aken, GA, Cohen Stuart, MA & Van de Velde, F 2006 Effect of droplet-matrix interactions on large deformation properties of emulsion-filled gels. Journal of Texture Studies 38 511535CrossRefGoogle Scholar
Sharma, R, Singh, H & Taylor, MW 1996 Comparison and structure of fat globule surface layers in recombined milk. Journal of Food Science 61 2832CrossRefGoogle Scholar
Tosh, S & Dalgleish, DG 1998 The physical properties and renneting characteristics of the synthetic membrane on the fat globules of microfluidized milk. Journal of Dairy Science 81 18401847CrossRefGoogle Scholar
van Kleef, FSM, Boskamp, JV & Van den Tempel, M 1978 Determination of the number of cross-links in a protein gel from its mechanical and swelling properties. Biopolymers 17 225235CrossRefGoogle Scholar
van Vliet, T 1988 Rheological properties of filled gels. Influence of filler matrix interaction. Colloid and Polymer Science 266 518524CrossRefGoogle Scholar
van Vliet, T & Dentener-Kikkert, A 1982 Influence of the milk fat globule membrane on the rheological properties of acid milk gels. Netherlands Milk and Dairy Science 36 61–265Google Scholar
Walstra, P 1990 On the stability of casein micelles. Journal of Dairy Science 73 1 19651979CrossRefGoogle Scholar
Wong, DWS, Camirand, WM & Pavlath, AE 1996 Structure and functionalities of milk proteins. Critical Reviews in Food Science and Nutrition 36:807844CrossRefGoogle ScholarPubMed
Xiong, YL & Kinsella, JE 1991 Influence of fat globule membrane composition and fat type on the rheological properties of milk based composite gels. II. Results. Milchwissenschaft 46 207211Google Scholar
Zoon, P, van Vliet, T & Walstra, P 1988 Rheological properties of rennet-induced skim milk gels. I. Introduction. Netherlands Milk and Dairy Science 42 249269Google Scholar