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In vivo nuclear magnetic resonance study of citrate metabolism in Propionibacterium freudenreichii subsp. shermanii

Published online by Cambridge University Press:  01 August 1998

CATHERINE DEBORDE ,
DOMINIQUE B. ROLIN ,
ARNAUD BONDON ,
JACQUES D. DE CERTAINES  and
PATRICK BOYAVAL
CATHERINE DEBORDE
Affiliation:
INRA, Laboratoire de Recherches de Technologie Laitière, 65 rue de Saint-Brieuc, F-35042 Rennes, France Institut Technique du Gruyère, 73 rue de Saint-Brieuc, BP 6224, F-35062 Rennes, France
DOMINIQUE B. ROLIN
Affiliation:
Université Victor Segalen, Bordeaux II, Laboratoire de Métabolisme et Développement des Plantes associé à INRA Bordeaux, BP 81, F-33883 Bordeaux, France
ARNAUD BONDON
Affiliation:
Laboratoire Chimie Organo-métallique et Biologique, UMR CNRS 6509, Campus de Beaulieu, Université Rennes I, F-35042 Rennes, France
JACQUES D. DE CERTAINES
Affiliation:
Laboratoire de Résonance Magnétique en Biologie et Médecine, UPRES EA 2230, Campus de Villejean, Université Rennes I, F-35043 Rennes, France
PATRICK BOYAVAL
Affiliation:
INRA, Laboratoire de Recherches de Technologie Laitière, 65 rue de Saint-Brieuc, F-35042 Rennes, France
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Abstract

Citrate metabolism by resting cells of Propionibacterium freudenreichii subsp. shermanii was investigated. In vivo13C nuclear magnetic resonance spectroscopy was used to study the pathway of citrate breakdown and to probe its utilization, non-invasively, in living cell suspensions. [2,4-13C]citrate was metabolized by resting cells to glutamate labelled in positions 2 and 4. In the presence of lactate or pyruvate, its rate of consumption was faster, but it was still converted to glutamate. No catabolic pathway other than the first third of a turn of the tricarboxylic acid cycle was used by Prop. freudenreichii subsp. shermanii to degrade citrate.

Type
Research Article
Information
Journal of Dairy Research , Volume 65 , Issue 3 , August 1998 , pp. 503 - 514
Copyright
Proprietors of Journal of Dairy Research 1998

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