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Immunological and electrophoretic study of the proteolytic enzymes from various Lactococcus and Lactobacillus strains

Published online by Cambridge University Press:  01 June 2009

Masahiro Sasaki ,
Boukje W. Bosman  and
Paris S. T. Tan
Masahiro Sasaki
Affiliation:
Snow Brand European Research Laboratories BV, Zernikepark 6, NL-9747 AN Groningen, The Netherlands
Boukje W. Bosman
Affiliation:
Snow Brand European Research Laboratories BV, Zernikepark 6, NL-9747 AN Groningen, The Netherlands
Paris S. T. Tan*
Affiliation:
Snow Brand European Research Laboratories BV, Zernikepark 6, NL-9747 AN Groningen, The Netherlands
*
*For correspondence.
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Summary

Cell extracts of various lactobacilli and two Lactococcus strains were investigated for their immunoresponse with monoclonal and polyclonal antibodies raised against various proteolytic enzymes from Lc. lactis. Except for Lactobacillus casei SBT 2233, none of the lactobacilli proteins showed immunoresponse with the monoclonal antibodies. With polyclonal antibodies raised against aminopeptidases N and C and endopeptidase of Lc. lactis an immunoresponse was observed. However, the molecular masses of the reactive bands on the blot were considerably different from those of the corresponding lactococcal peptidases, except for the band that reacted with polyclonal antibodies against aminopeptidase C. The polyclonal antibodies raised against X-prolyl-dipeptidyl aminopeptidase and tripeptidase did not show any immunoreaction. As a control, all antibodies reacted with the lactococcal proteins on the blot, with molecular masses corresponding to those reported for the proteinases and peptidases. The results clearly showed that most of the proteolytic enzymes of lactobacilli were immunologically different from those of lactococci. The proteolytic enzymes in the cell-free extracts were separated by non-denaturing PAGE and visualized by zymogram staining. The electrophoretic pattern of the proteolytic enzymes of lactobacilli was different from that of Lc. lactis. Both experiments indicate that the enzymes of the proteolytic system of lactobacilli are different from those of lactococci.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 4 , November 1995 , pp. 611 - 620
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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