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A fractionation of the αs-casein complex of bovine milk

  • W. D. Annan (a1) and W. Manson (a1)

Summary

The nature of the αs-casein complex of bovine milk has been investigated by cation-exchange chromatography on columns of sulphoethyl Sephadex C-50 of purified preparations of αs-casein and of whole acid-precipitated casein. Three main fractions were separated from each. Two behaved as single homogeneous proteins as judged by starch-gel electrophoresis; they were the main constituent of the complex, αs1-casein, and a closely related phosphoprotein, designated αs0-casein, which was present in small amounts only. The third fraction also constituted only a small part of the total complex. It was heterogeneous on starch gel electrophoresis and contained 2 major and 2 minor components. This fraction, while similar to other members of the αs-casein complex and to β-caseins hitherto described in that it contained phosphorus, nevertheless differed significantly from these since 3 at least of its components contained sulphur either in the form of disulphide bonds or of sulphydryl groupings.

Information is presented on the composition of the 3 fractions including C-terminal end group analysis.

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References

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Allen, R. J. L. (1940). Biochem. J. 34, 858.
Anfinsen, C. B. & Haber, E. (1961). J. biol. Chem. 236, 1361.
Aronsson, T. & Grönwall, A. (1957). Scand. J. clin. Lab. Invest. 9, 338.
Aschaffenburg, R. & Drewry, J. (1957). Biochem. J. 65, 273.
El-Negoumy, A. M. (1967). Biochim. biophys. Acta 140, 503.
Haber, E. & Anfinsen, C. B. (1961). J. biol. Chem. 236, 422.
Lowry, D. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). J. biol. Chem. 193, 265.
MacKinlay, A. G. & Wake, R. G. (1965). Biochim. biophys. Acta 104, 167.
McMeekin, T. L., Hipp, N. J. & Groves, M. L. (1959). Archs Biochem. Biophys. 83, 35.
Manson, W. (1961). Archs Biochem. Biophys. 95, 336.
Manson, W. (1962). Biochim. biophys. Acta 63, 515.
Manson, W. (1965). Biochem. J. 94, 452.
Marier, J. R. & Rose, D. (1964). Analyt. Biochem. 7, 304.
Neelin, J. M. (1964). J. Dairy Sci. 47, 506.
Schmidt, D. G., Payens, T. A. J., van Markwijk, B. W. & Brinkhuis, J. A. (1967). Biochem. biophys. Res. Commun. 27, 448.
Smithies, O. (1959). Biochem. J. 71, 585.
Spackman, D. H., Stein, W. H. & Moore, S. (1958). Analyt. Chem. 30, 1190.
Thompson, M. P. (1966). J. Dairy Sci. 49, 792.
Thompson, M. P. & Kiddy, C. A. (1964). J. Dairy Sci. 47, 626.
Thompson, M. P., Kiddy, C. A., Pepper, L. & Zittle, C. A. (1962). Nature, Lond. 195, 1001.
Thompson, M. P., Tarassuk, N. P., Jenness, R., Lillevik, H. A., Ashworth, U. S. & Rose, D. (1965). J. Dairy Sci. 48, 159.
Waugh, D. F., Ludwig, M. L., Gillespie, J. M., Melton, B., Foley, M. & Kleiner, E. S. (1962). J. Am. Chem. Soc. 84, 4929.
Waugh, D. F. & von Hippel, P. H. (1956). J. Am. Chem. Soc. 78, 4576.
Weil, L. & Seibles, T. S. (1961). Archs Biochem. Biophys. 93, 193.

A fractionation of the αs-casein complex of bovine milk

  • W. D. Annan (a1) and W. Manson (a1)

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