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Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows

Published online by Cambridge University Press:  24 February 2021

Brian Christensen ,
Elias D. Zachariae ,
Nina A. Poulsen ,
Albert J. Buitenhuis ,
Lotte B. Larsen  and
Esben S. Sørensen
Brian Christensen
Affiliation:
Department of Molecular Biology and Genetics, Science Park, Aarhus University, Aarhus, Denmark CiFOOD, Aarhus University Centre for Innovative Food Research, Aarhus, Denmark
Elias D. Zachariae
Affiliation:
Department of Molecular Biology and Genetics, Science Park, Aarhus University, Aarhus, Denmark
Nina A. Poulsen
Affiliation:
CiFOOD, Aarhus University Centre for Innovative Food Research, Aarhus, Denmark Department of Food Science, Aarhus University, Aarhus, Denmark
Albert J. Buitenhuis
Affiliation:
CiFOOD, Aarhus University Centre for Innovative Food Research, Aarhus, Denmark Center for Quantitative Genetics and Genomics, Aarhus University, Tjele, Denmark
Lotte B. Larsen
Affiliation:
CiFOOD, Aarhus University Centre for Innovative Food Research, Aarhus, Denmark Department of Food Science, Aarhus University, Aarhus, Denmark
Esben S. Sørensen*
Affiliation:
Department of Molecular Biology and Genetics, Science Park, Aarhus University, Aarhus, Denmark CiFOOD, Aarhus University Centre for Innovative Food Research, Aarhus, Denmark
*
Author for correspondence: Esben S. Sørensen, Email: ess@mbg.au.dk
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Abstract

Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk production traits and lactation persistency in cows. In the present study, we report the development of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/l with a pronounced level of individual variation ranging from 0.4 mg/l to 67.8 mg/l. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.

Keywords

Bioactive milk proteinELISAheritabilityOsteopontin
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 1 , February 2021 , pp. 89 - 94
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Ashkar S, W, Panoutsakopoulou V, GF, Sanchirico, ME, Jansson, M, Zawaideh, S, Rittling, SR, Denhardt, DT, Glimcher, MJ and Cantor, H (2000) Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. Science (New York, N.Y.) 287, 860–864.CrossRefGoogle ScholarPubMed
Azuma, N, Maeta, A, Fukuchi, K and Kanno, C (2006) A rapid method for purifying osteopontin from bovine milk and interaction between osteopontin and other milk proteins. International Dairy Journal 16, 370–378.CrossRefGoogle Scholar
Bissonnette, N (2018) Short communication: genetic association of variations in the osteopontin gene (SPP1) with lactation persistency in dairy cattle. Journal of Dairy Science 101, 456–461.CrossRefGoogle ScholarPubMed
Boskey A, L, Christensen, B, Taleb, H and Sørensen, ES (2012) Post-translational modification of osteopontin: effects on in vitro hydroxyapatite formation and growth. Biochemical and Biophysical Research Communications 419, 333–338.Google ScholarPubMed
Bruun, S, Jacobsen, LN, Ze, X, Husby, S, Ueno, HM, Nojiri, K, Kobayashi, S, Kwon, J, Liu, X, Yan, S, Yang, J, Zachariassen, G, Chen, L, Zhou, W, Christensen, B and Sørensen, ES (2018) Osteopontin levels in human milk vary across countries and within lactation period: data from a multicenter study. Journal of Pediatric Gastroenterology and Nutrition 67, 250–256.Google ScholarPubMed
Chatterton, DEW, Rasmussen, JT, Heegaard, CW, Sørensen, ES and Petersen, TE (2004) In vitro digestion of novel milk protein ingredients for use in infant formulas: research on biological functions. Trends in Food Science & Technology 15, 373–383.CrossRefGoogle Scholar
Christensen, B and Sørensen, ES (2014) Osteopontin is highly susceptible to cleavage in bovine milk and the proteolytic fragments bind the αVβ₃-integrin receptor. Journal of Dairy Science 97, 136–146.CrossRefGoogle ScholarPubMed
Christensen, B and Sørensen, ES (2016) Structure, function and nutritional potential of milk osteopontin. International Dairy Journal 57, 1–6.CrossRefGoogle Scholar
Christensen, B, Nielsen, MS, Haselmann, KF, Petersen, TE and Sørensen, ES (2005) Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications. The Biochemical Journal 390(Pt 1), 285–292.CrossRefGoogle ScholarPubMed
Christensen, B, Karlsen, NJ, Jørgensen, SDS, Jacobsen, LN, Ostenfeld, MS, Petersen, SV, Müllertz, A and Sørensen, ES (2020) Milk osteopontin retains integrin-binding activity after in vitro gastrointestinal transit. Journal of Dairy Science 103, 42–51.CrossRefGoogle ScholarPubMed
Donovan, SM, Monaco, MH, Drnevich, J, Kvistgaard, AS, Hernell, O and Lönnerdal, B (2014) Bovine osteopontin modifies the intestinal transcriptome of formula-fed infant rhesus monkeys to be more similar to those that were breastfed. The Journal of Nutrition 144, 1910–1919.CrossRefGoogle ScholarPubMed
Dudemaine, PL, Thibault, C, Alain, K and Bissonnette, N (2014) Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk. Animal Genetics 45, 629–640.CrossRefGoogle ScholarPubMed
Gebreyesus, G, Lund, MS, Buitenhuis, B, Bovenhuis, H, Poulsen, NA and Janss, LG (2017) Modeling heterogeneous (co)variances from adjacent-SNP groups improves genomic prediction for milk protein composition traits. Genetics, Selection, Evolution: GSE 49, 89.CrossRefGoogle ScholarPubMed
Khatib, H, Zaitoun, I, Wiebelhaus-Finger, J, Chang, YM and Rosa, GJM (2007) The association of bovine PPARGC1A and OPN genes with milk composition in two independent Holstein cattle populations. Journal of Dairy Science 90, 2966–2970.CrossRefGoogle ScholarPubMed
Leonard, S, Khatib, H, Schutzkus, V, Chang, YM and Maltecca, C (2005) Effects of the osteopontin gene variants on milk production traits in dairy cattle. Journal of Dairy Science 88, 4083–4086.Google ScholarPubMed
Lok, ZSY and Lyle, AN (2019) Osteopontin in vascular disease. Arteriosclerosis. Thrombosis and Vascular Biology 39, 613–622.CrossRefGoogle Scholar
Lönnerdal, B, Kvistgaard, AS, Peerson, JM, Donovan, SM and Peng, Y-M (2015) Growth, nutrition and cytokine response of breast-fed infants and infants fed formula with added bovine osteopontin. Journal of Pediatric Gastroenterology and Nutrition 62, 650–657.CrossRefGoogle Scholar
Madsen, P and Jensen, J (2013) A user's guide to DMU. A Package for Analysing Multivariate Mixed Models Version 6, 1–33.Google Scholar
Poulsen, NA, Gustavsson, F, Glantz, M, Paulsson, M, Larsen, LB and Larsen, MK (2012) The influence of feed and herd on fatty acid composition in 3 dairy breeds (Danish Holstein, Danish Jersey and Swedish Red). Journal of Dairy Science 95, 6362–6371.CrossRefGoogle Scholar
Schack, L, Lange, A, Kelsen, J, Agnholt, J, Christensen, B, Petersen, TE and Sørensen, ES (2009a) Considerable variation in the concentration of osteopontin in human milk, bovine milk, and infant formulas. Journal of Dairy Science 92, 5378–5385.CrossRefGoogle Scholar
Schack, L, Stapulionis, R, Christensen, B, Kofod-Olsen, E, Skov Sørensen, UB, Vorup-Jensen, T, Sørensen, ES and Höllsberg, P (2009b) Osteopontin enhances phagocytosis through a novel osteopontin receptor, the alphaXbeta2 integrin. Journal of Immunology 182, 6943–6950.CrossRefGoogle Scholar
Sheehy, PA, Riley, LG, Raadsma, HW, Williamson, P and Wynn, PC (2009) A functional genomics approach to evaluate candidate genes located in a QTL interval for milk production traits on BTA6. Animal Genetics 40, 492–498.CrossRefGoogle Scholar
Sørensen, ES and Petersen, TE (1993) Purification and characterization of three proteins isolated from the proteose peptone fraction of bovine milk. The Journal of Dairy Research 60, 189–197.CrossRefGoogle ScholarPubMed
Sørensen, ES, Højrup, P and Petersen, TE (1995) Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sites. Protein Science: A Publication of the Protein Society 4, 2040–2049.CrossRefGoogle ScholarPubMed
Strucken, EM, Laurenson, YCSM and Brockmann, GA (2015) Go with the flow-biology and genetics of the lactation cycle. Frontiers in Genetics 6, 118.Google ScholarPubMed
VanRaden, PM (2008) Efficient methods to compute genomic predictions. Journal of Dairy Science 91, 4414–4423.CrossRefGoogle ScholarPubMed
Wilmink, JBM (1987) Adjustment of test-day milk, fat and protein yield for age, season and stage of lactation. Livestock Production Science 16, 335–348.CrossRefGoogle Scholar
Yamniuk, AP, Burling, H and Vogel, HJ (2009) Thermodynamic characterization of the interactions between the immunoregulatory proteins osteopontin and lactoferrin. Molecular Immunology 46, 2395–2402.CrossRefGoogle ScholarPubMed