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Effect of trypsin on bovine lactoferrin and interaction between the fragments under different conditions

  • Luis Mata (a1), Helena Castillo (a1), Lourdes Sánchez (a1), Pilar Puyol (a1) and Miguel Calvo (a1)...


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Bellamy, W., Takase, M., Yamauchi, K., Wakabayashi, H., Kawase, K. & Tomita, M. 1992 Identification of the bactericidal domain of lactoferrin. Biochimica et Biophysica Acta 1121 130136
Bennett, R. M., Bagby, G. C. & Davis, J. 1981 Calcium-dependent polymerization of lactofurrin. Biochemical and Biophysical Research Communications 101 8895
Brines, R. D. & Brock, J. H. 1983 The effect of trypsin and chymotrypsin on the in vitro antimicrobial and iron-binding properties of lactoferrin in human milk and bovine colostrum. Unusual resistance of human apolactoferrin to proteolytic digestion. Biochimica et Biophysica Acta 759 229235
Brock, J. H., Arzabe, F., Lampreave, F. & Piñeiro, A. 1976 The effect of trypsin on bovine transferrin and lactoferrin. Biochimica et Biophysica Acta 446 214225
Butler, J. E. 1973 The occurrence of immunoglobulin fragments, two types of lactoferrin and a lactoferrin-IgG2 complex in bovine colostral and milk whey. Biochimica et Biophysica Acta 295 341351
Jorieux, S., Mazurier, J., Montreuil, J. & Spik, G. 1985 Characterization of lactotransferrin complexes in human milk. Protides of the Biological Fluids 32 115118
Lampreave, F., Piñeiro, A., Brock, J. H., Castillo, H., Sánchez, L. & Calvo, M. 1990 Interaction of bovine lactoferrin with other proteins of milk whey. International Journal of Biological Macromolecules 12 25
Legrand, D., Mazurier, J., Aubert, J. P., Loucheux-Lefebvre, M. H., Montreuil, J. & Spik, G. 1986 Evidence for interactions between the 30 kDa N- and 50 kDa C-terminal tryptic fragments of human lactotransferrin. Biochemical Journal 236 839844
Legrand, D., Mazurier, J., Colavizza, D., Montreuil, J. & Spik, G. 1990 Properties of the iron-binding site of the N-terminal lobe of human and bovine lactotransferrins. Importance of the glycan moiety and of the non-covalent interactions between the N- and C-terminal lobes in the stability of the iron-binding site. Biochemical Journal 266 575581
Line, W. F., Sly, D. A. & Bezkorovainy, A. 1976 Limited cleavage of human lactoferrin with pepsin. International Journal of Biochemistry 7 203208
Sánchez, L., Aranda, P., Pérez, M.-D. & Calvo, M. 1988 Concentration of lactoferrin and transferrin throughout lactation in cow's colostrum and milk. Biological Chemistry Hoppe-Seyler 369 10051008
Sánchez, L., Calvo, M. & Brock, J. H. 1992 Biological role of lactoferrin. Archives of Disease in Childhood 67 657661
Tomita, M., Bellamy, W., Takase, M., Yamauchi, K., Wakabayashi, H. & Kawase, K. 1991 Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. Journal of Dairy Science 74 41374142
Watanabe, T., Nagura, H., Watanabe, K. & Brown, W. R. 1984 The binding of human milk lactoferrin to immunoglobulin A. FBBS Letters 168 203207

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Effect of trypsin on bovine lactoferrin and interaction between the fragments under different conditions

  • Luis Mata (a1), Helena Castillo (a1), Lourdes Sánchez (a1), Pilar Puyol (a1) and Miguel Calvo (a1)...


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