Skip to main content Accessibility help
×
Home

The dynamics of individual whey protein concentrations in cows’ mammary secretions during the colostral and early lactation periods

  • Raquel F.S. Raimondo (a1), Juliana S.P. Ferrão (a2), Samantha I. Miyashiro (a2), Priscila T. Ferreira (a1), João Paulo E. Saut (a3), Daniela B. Birgel (a4) and Eduardo H. Birgel Junior (a4)...

Abstract

The bovine whey consists of more than 200 different types of proteins, of which β-lactoglobulin, α-lactalbumin, serum albumin, immunoglobulins and lactoferrin predominate. However, their concentrations are not stable due to the existence of protein dynamics during a transition from colostrum secretion to mature milk. To evaluate the dynamics of whey proteins of Jersey cows during a colostral phase and first month of lactation and an influence of the number of lactations, 268 milk samples from 135 Jersey cows were selected through a clinical evaluation. Whey was obtained by rennet coagulation of the mammary secretion. The concentration of total proteins was determined by the biuret method and their fractions were identified by 12% dodecyl sulfate-polyacrylamide gel electrophoresis (12% SDS-PAGE). Maximum concentrations of all protein fractions were observed in the first 12 h of lactation, reducing over the course of the study. Modification of the protein predominance was also observed. The transition from colostrum secretion to milk occurred between 24 and 72 h postpartum. There was an influence of the number of lactations on the dynamics of whey proteins, indicating that multiparous cows had better immunological and nutritional quality when compared to primiparous cows.

Copyright

Corresponding author

Authors for correspondence: Raquel F.S. Raimondo, Email: rfraimondo@gmail.com

References

Hide All
Chigerwe, M, Dawes, ME, Tyler, JW, Middleton, JR, Moore, MP and Nagy, DM (2005) Evaluation of a cow-side immunoassay kit for assessing IgG concentration in colostrum. Journal of American Veterinary Medical Association 227, 129131.
Costa, EO, Melville, PA, Ribeiro, AR, Watanabe, E, Viani, FC and White, CR (1996) Prevalence of intramammary infections in primigravid Brazilian dairy heifers. Preventive Veterinary Medicine 29, 151155.
Dirksen, G, Gründer, H-D and Stöber, M (1993) Rosenberg Die Klinische Untersuchung des Rindes. 3rd ed. Berlin and Hamburg: Verlagbuchhandlung Paul Parey.
Guilloteau, P, Romé, V, Delaby, L, Mendy, F, Roger, L and Chayvialle, JA (2009) A new role of phosphopeptides as bioactive peptides released during milk casein digestion in the young mammal: regulation of gastric secretion. Peptides 30, 22212227.
Heng, GB (1999) Chemical composition of bovine colostrums. Pages 405–411 in International Conference of Food Science and Technology. Anais, Trumbull, CT Trumbull: Food and Nutrition Press.
Hillier, RM (1976) The quantitative measurement of whey proteins using polyacrylamide-gel electrophoresis. Journal of Dairy Research 43, 259265.
Hiss, S, Meyer, T and Sauerwein, H (2008) Lactoferrin concentrations in goat milk throughout lactation. Small Ruminant Research 80, 8790.
Króls, J, Brodziak, A, Litwińczuk, Z and Litwińczuk, A (2013) Effect of age and stage of lactation on whey protein content in milk of cows of different breeds. Polish Journal of Veterinary Science 16, 395397.
Levieux, D and Ollier, A (1999) Bovine immunoglobulin G, beta-lactoglobulin, alpha-lactalbumin and serum albumin in colostrum and milk during the early post partum period. Journal of Dairy Research 66, 421430.
Madsen, BD, Rasmussen, MD, Nielsen, MO, Wiking, L and Larsen, LB (2004) Physical properties of mammary secretions in relation to chemical changes during transition from colostrum to milk. Journal of Dairy Research 71, 263272.
McGrath, BA, Fox, PF, Mcsweeney, PLH and Kelly, L (2016) Composition and properties of bovine colostrum: a review. Dairy Science Technology 96, 133158.
Nguyen, DD, Neville, MC (1998) Tight junction regulation in the mammary gland. Journal of Mammary Gland Biology and Neoplasia 3, 233246.
Phillippy, BO, Mccarthy, RD (1979) Multi-origins of milk serum albumin in the lactating goat. Biochimica Biophysica Acta 584, 298303.
Raimondo, RFS, Mori, CS, Miyiashiro, SI and Junior, EHB (2010 a) Standardization of the Biuret Method for determining the whey proteins. Page 119 in World Buiatrics Congress. Proc., Santiago. Santiago: World Association for Buiatrics.
Raimondo, RFS, Pogliani, FC, Birgel, DB, Saut, JPE and Birgel, EH Jr (2010 b) Whey protein profile from heifers and multiparous Jersey's cows during the early lactation. Page 254 in World Buiatrics Congress. Proc., Santiago. Santiago: World Association for Buiatrics.
Raimondo, RFS, Brandespim, FB, Prina, APM, Miyashiro, SI, Saut, JPE, Mori, CS, Pogliani, FC and Birgel, EH (2013 a) Dynamic in the concentration of whey proteins in the mammary secretion of goats during the dry period. Small Ruminant Research 113, 239246.
Raimondo, RFS, Miyiashiro, SI, Mori, CS and Birgel Junior, EH (2013 b) Proteínas do soro lácteo de vacas da raça Jersey durante a lactação. Pesquisa Veterinária Brasileira 33, 119125.
Senda, A, Fukuda, K, Ishii, T and Urashima, T (2011) Changes in the bovine whey proteome during the early lactation period. Animal Science Journal 82, 698706.
Tenhagen, BA, Hansen, I, Reinecke, A and Heuwieser, W (2009) Prevalence of pathogens in milk samples of dairy cows with clinical mastitis and in heifers at first parturition. Journal of Dairy Research 76, 179187.
Tsuji, S, Hirata, Y, Mukai, F and Ohtagaki, S (1990) Comparison of lactoferrin content in colostrum between different cattle breeds. Journal of Dairy Science 73, 125128.
Yoshida, S, Wei, Z, Shinmura, Y and Fukunaga, N (2000) Separation of lactoferrin-a and -b from bovine colostrum. Journal of Dairy Science 83, 22112215.
Zhang, LY, Wang, JQ, Yang, YX, Bu, DP, Li, SS and Zhou, LY (2011) Comparative proteomic analysis of changes in the bovine whey proteome during the transition from colostrum to milk. Asian-australas. Journal of Animal Science 24, 272278.

Keywords

The dynamics of individual whey protein concentrations in cows’ mammary secretions during the colostral and early lactation periods

  • Raquel F.S. Raimondo (a1), Juliana S.P. Ferrão (a2), Samantha I. Miyashiro (a2), Priscila T. Ferreira (a1), João Paulo E. Saut (a3), Daniela B. Birgel (a4) and Eduardo H. Birgel Junior (a4)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed