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Detection of proteolysis in raw milk stored at low temperature by an inhibition ELISA

Published online by Cambridge University Press:  01 June 2009

Catherine Picard ,
Isabelle Plard ,
Dominique Rongdaux-Gaida  and
Jean-Claude Collin
Catherine Picard
Affiliation:
LCB Laboratoire de Chimie et de Biologie, 71260 La Salle, France
Isabelle Plard
Affiliation:
INRA Station de Recherches en Technologie et Analyses Laiti`res, BP 89, 39801 Poligny Cedex, France
Dominique Rongdaux-Gaida
Affiliation:
INRA Station de Recherches en Technologie et Analyses Laiti`res, BP 89, 39801 Poligny Cedex, France
Jean-Claude Collin
Affiliation:
INRA Station de Recherches en Technologie et Analyses Laiti`res, BP 89, 39801 Poligny Cedex, France
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Summary

An inhibition ELISA (enzyme-linked immunosorbent assay) was developed for the determination of caseinomacropeptide (CMP) in order to estimate the proteolysis of κ-casein due to the enzymes of psychrotrophic bacteria in bulk raw milk. The CMP present in milk was quantified specifically by an antibody. The limit of detection was ∽ 0·1 μg/ml and the CV was < 10%. This method was used to study the proteolytic activity of three strains of psychrotrophic Pseudomonas fluorescens in raw milk and to analyse different raw milk samples supplied by four dairy plants. The proteolytic activity for different strains of psychrotrophs and for different milk samples varied considerably, but no correlation was established between the level of microbial flora and κ-casein proteolysis. It is thus not possible to determine the extent of proteolysis from the bacterial count alone. However, by CMP determination in bulk raw milk samples after 6 d storage at 4°C, the mean κ-casein proteolysis was ∽ 4%. Among the milk samples analysed that contained < 107 cfu psychrotrophs/ml, 30% exhibited a proteolysis of κ-casein < 0·5%, i.e. < 5μg CMP/ml.

Type
Original Articles
Information
Journal of Dairy Research , Volume 61 , Issue 3 , August 1994 , pp. 395 - 404
Copyright
Copyright © Proprietors of Journal of Dairy Research 1994

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