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Contribution of plasmin to Cheddar cheese ripening: effect of added plasmin

  • Nana Farkye (a1) and Patrick F. Fox (a1)

Summary

Plasmin (EC 3.4.21.7) was added to cheese milk to assess its contribution to Cheddar cheese ripening; the activity of plasmin in the cheese was increased by levels ranging from 1·5 to 6 times that in the control cheeses. Even at the highest level of added plasmin, no activity was found in the whey. β-Casein was degraded faster in the experimental cheeses than in the controls, and the concentration of γ-caseins increased concomitantly. The total N in the water-soluble extract was up to ˜ 20% higher in the experimental than in the control cheeses but phosphotungstic acid-soluble N was not affected by the plasmin activity in the cheese. Several differences were apparent in the gel electropherograms of the water-soluble extracts of the experimental and control cheeses; some peptides were present at higher concentrations in the former, others in the latter, suggesting that plasmin contributes to both the formation and degradation of water-soluble peptides in cheese. The organoleptic quality of the plasmin-enriched cheeses was judged superior to that of the controls and ripening was considerably accelerated; a plasmin level 3–4 times the indigenous value appeared to be optimal. No bitterness was detected in any of the cheeses.

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References

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Andrews, A. T. 1983 Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research 50 4555
Association Of Official Analytical Chemists 1980 Official Methods of Analysis, 13th edn (Ed. Horwitz, W.) Washington, DC: AOAC
Chen, J. H. & Ledford, R. A. 1971 Purification and characterization of milk protease. Journal of Dairy Science 54 763 (Abstr.)
Creamer, L. K. 1975 β-Casein degradation in Gouda and Cheddar cheese. Journal of Dairy Science 58 287292
Eigel, W. N. 1977 a Effect of bovine plasmin on αs1-B and κ-A caseins. Journal of Dairy Science 60 13991403
Eigel, W. N. 1977 b Formation of γ1,-A2, γ2-A2 and γ3-A caseins by in vitro proteolysis of κ-casein A2 with bovine plasmin. International Journal of Biochemistry 8 187192
Fox, P. F. 1963 Potentiometric determination of salt in cheese. Journal of Dairy Science 46 744745
Fox, P. F. 1989 Acceleration of cheese ripening. Food Biotechnology 2 133185
Grappin, R., Rank, T. C. & Olson, N. F. 1985 Primary proteolysis of cheese proteins during ripening. A review. Journal of Dairy Science 68 531540
Grieve, P. A. & Dulley, J. R. 1983 Use of Streptococcus lactis lac mutants for accelerating Cheddar cheese ripening. 2. Their effect on the rate of proteolysis and flavour development. Australian Journal of Dairy Technology 38 4954
Grufferty, M. B. & Fox, P. F. 1988 a Milk alkaline proteinase. Review article Journal of Dairy Research 55 609630
Grufferty, M. B. & Fox, P. F. 1988 b Functional properties of casein hydrolysed by alkaline milk proteinase. New Zealand Journal of Dairy Science and Technology 23 95108
Jarrett, W. D., Aston, J. W. & Dulley, J. R. 1982 A simple method for estimating free amino acids in Cheddar cheese. Australian Journal of Dairy Technology 37 5558
Kaminogawa, S., Mizobuchi, H. & Yamauchi, K. 1972 Comparison of bovine milk protease with plasmin. Agricultural and Biological Chemistry 36 21632167
Kuchroo, C. N. & Fox, P. F. 1982 Soluble nitrogen in Cheddar cheese: comparison of extraction procedures. Milchwissenschaft 37 331335
Lawrence, R. C., Creamer, L. K. & Gilles, J. 1987 Texture development during cheese ripening. Journal of Dairy Science 70 17481760
Le Bars, D. & Gripon, J.-C. 1989 Specificity of plasmin towards bovine αs2-casein. Journal of Dairy Research 56 817821
O'Keeffe, R. B., Fox, P. F. & Daly, C. 1976 Contribution of rennet and starter proteases to proteolysis in Cheddar cheese. Journal of Dairy Research 43 97107
Ollikainen, P. & Nyberg, K. 1988 A study of plasmin activity during ripening of Swiss-type cheese. Milchwissenschaft 43 497499
Pearse, M. J., Linklater, P. M., Hall, R. J. & MacKinlay, A. G. 1986 Extensive degradation of casein by plasmin does not impede subsequent curd formation and syneresis. Journal of Dairy Research 53 477480
Richardson, B. C. & Pearce, K. N. 1981 The determination of plasmin in dairy products. New Zealand Journal of Dairy Science and Technology 16 209220
Scott, R. 1986 Cheesemaking Practice, 2nd edn.London: Elsevier Applied Science Publishers
Shalabi, S. I. & Fox, P. F. 1987 Electrophoretic analysis of cheese: comparison of methods. Irish Journal of Food Science and Technology 11 135151
Visser, F. M. W. & De Groot-Mostert, A. E. A. 1977 Contribution of enzymes from rennet, starter bacteria and milk to proteolysis and flavour development in Gouda cheese. 4. Protein breakdown: a gel electrophoretic study. Netherlands Milk and Dairy Journal 31 247264
Visser, S., Slangen, K. J., Hup, G. & Stadhouders, J. 1983 Bitter flavour in cheese. 3. Comparative gel-chromatographic analysis of hydrophobic peptide fractions from twelve Gouda-type cheeses and identification of bitter peptides isolated from a cheese made with Streptococcus cremoris strain HP. Netherlands Milk and Dairy Journal 37 181192

Contribution of plasmin to Cheddar cheese ripening: effect of added plasmin

  • Nana Farkye (a1) and Patrick F. Fox (a1)

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