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Comparison of a gelation and a chromogenic Limulus (LAL) assay for the detection of Gram-negative bacteria, and the application of the latter assay to milk

Published online by Cambridge University Press:  01 June 2009

Annelie Svensson  and
Bärbel Hahn-Hägerdal
Annelie Svensson
Affiliation:
Applied Microbiology, Chemical Center, Land University, Box 124, S-221 00 Lund, Sweden
Bärbel Hahn-Hägerdal
Affiliation:
Applied Microbiology, Chemical Center, Land University, Box 124, S-221 00 Lund, Sweden
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Summary

When a chromogenic Limulus Amoebocyte Lysate (LAL) assay and a tube gelation LAL assay were compared for the detection of Gram-negative bacteria, using a strain of Pseudomonas putida, the detection level (∼ 103 cfu/ml) and cost of the assays were approximately the same for both assays but the reading was more precise for the chromogenic substrate assay. A modified chromogenic assay was devised for detection of Ps. putida in milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 2 , May 1987 , pp. 267 - 273
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

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