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Characterization and kinetics of gastric emptying of peptides derived from milk proteins in the preruminant calf

  • P. Scanff (a1), M. Yvon (a1), S. Thirouin (a1) and J.-P. Pelissffir (a1)


The gastric emptying kinetics of peptides derived from milk protein were studied in vivo in preruminant calves by collecting and characterizing the whole effluent leaving the stomach for 12 h after ingestion of crude skim milk. Peptides were isolated by reversed-phase HPLC and identified. Particular attention was paid to biologically active peptides and to peptides that could be precursors of biologically active sequences. A gastrin inhibitor, the caseinomacropeptide, was emptied from the stomach only during the first 0·5 h of digestion and rapidly hydrolysed. Precursors of immunostimulatory peptides from αs1 - and β-caseins were emptied throughout digestion in the gastric effluent. A precursor of β-casomorphins (peptide 58–93 of β-casein) was emptied from the stomach 3·5 h after the meal when it was taken on an empty stomach. From this precursor, peptides that may be resistant to hydrolysis by intestinal peptidase were obtained after in vitro hydrolysis by pancreatic enzymes. A phosphopeptide (fragment 110–142 of αs1-casein) was also found in digesta after a few hours of digestion. When the meal was not taken on an empty stomach, these peptides were emptied in the first digesta at a low concentration. The potential activity of these peptides is discussed. The results support the hypothesis that active sequences could still be present in the gut after the action of pancreatic enzymes.



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Aleiˇnik, S. T., Stan, E. Ya. & Chernikov, M. P. 1984 [A glycopeptide obtained from κ-casein and its effect on Protein assimilation.] Voprosy Pitaniya no. II 4750
Ash, R. W. 1964 Abomasal secretion and emptying in suckled calves. Journal of Physiology 172 425438
Beucher, S. 1989 [Determination of the specificity of clastase for αs1- and β-caseins.] Mémoire de DEA Sciences Alimentaires. Université Paris 7, France
Brantl, V., Teschemacher, H., Henschen, A. & Lottspeich, F. 1979 Novel opioid peptidcs derived from casein (β-casomorphins). 1. Isolation from bovine casein peptone. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 360 12111216
Brust, P., Köhler, R., Brandsch, M., Born, I., Hartrodt, B., Neubert, K. & Gebhardt, G. 1991 Effects of dietary β-casomorphins on the intestinal transport of amino acids and on plasma insulin concentrations in rats. Zoologisches Jahrblatt für Physiologie 95 97107
Caporale, C., Fontanella, A., Petrilli, P., Pucci, P., Molinaro, M. F., Picone, D. & Auricchio, S. 1985 Isolation and characterization of dipeptidyl peptidase IV from human meconium. FEBS Letters 184 273277
Chernikov, M. P., Nikol'Skaya, G. V., Stan, E. Ya., Shlygin, G. K. & Vasilevskaya, L. S. 1974 [Biological role of casein glycomacropeptide.] Voprosy Pitaniya no. II 2125
Daniel, H., Vohwinkel, M. & Rehner, G. 1990 Effect of casein and β-casomorphins on gastrointestinal motility in rats. Journal of Nutrition 120 252257
Fiat, A.-M., Levy-Toledano, S., Caen, J. P. & Jollés, P. 1989 Biologically active peptides of casein and lactotransferrin implicated in platelet function. Journal of Dairy Research 56 351355
Gerber, H. W. & Jost, R. 1986 Casein phosphopeptides: their effect on calcification of in vitro cultured embryonic rat bone. Calcified Tissue International 38 350357
Guilloteau, P., Chayvialle, J. A., Mendy, F., Roger, L., Toullec, R., Bernard, C., Mouats, A. & Favbrdin, P. 1986 [Effects of caseinomacropeptide on gastric secretion and circulating levels of digestive hormones in the preruminant calf.] 2-ème Journées Nutrition des Herbivores
Guillou, H., Miranda, G. & Pélissier, J.-P. 1991 Hydrolysis of β-casein by gastric proteasos. 1. Comparison of proteolytic action of bovine chymosin and pepsin A. International Journal of Peptide and Protein Research 37 494501
Henschen, A., Lottspeich, F., Brantl, V. & Teschemacher, H. 1979 Novel opioid peptidcs derived from casein (β-casomorphins). II. Structure of active components from bovine casein peptone. Hoppe Seyler's Zeitschrift für Physiologische Chemie 360 12171224
Jollés, P., Levy-Toledano, S., Fiat, A.-M., Soria, C., Gillessen, D., Thomaidis, A., Dunn, F. W. & Caen, J. P. 1986 Analogy between fibrinogen and casein. Effect of an undecapeptide isolated from k-casein on platelet function. European Journal of Biochemistry 158 379382
Kaminogawa, S. 1981 [Mechanism of casein degradation by proteases.] Japnnese Journal of Zootechnical Science 52, 627638
Lee, Y. S., Noguchi, T. & Naito, H. 1979 An enhanced intestinal absorption of calcium in the rat directly attributed to dietary casein. Agricultural and Biological Chemistry 43 20092011
Léonil, J. & Mollé, D. 1990 Liberation of tryptic fragments from caseinomacropeptide of bovine k-casein involved in platelet function. Kinetic study. Biochemical Journal 271 247252
Lopker, A., Abood, L. G., Hoss, W. & Lionetti, F. J. 1980 Stereoselective muscarinic, acetylcholine and opiate receptors in human phagocytic leukocytes. Biochemical Pharmacology 29 13611365
Maruyama, S., Mitachi, H., Tanaka, H., Tomizuka, N. & Suzuki, H. 1987 Studies on the active site and antihypertensive activity of angiotensin-1 converting enzyme inhibitors derived from casein. Agricultural and Biological Chemistry 51 15811586
Maruyama, S., Nakagomi, K., Tomizuka, N. & Suzuki, H. 1985 Angiotensin I converting enzyme inhibitor derived from an enzymatic hydrolysate of casein. II. Isolation and bradykinin-potentiating activity on the uterus and the ileum of rats. Agricultural and Biological Chemistry 49 14051409
Meisel, H. & Frister, H. 1988 Chemical characterization of a caseinophosphopeptide isolated from in vivo digests of a casein diet. Biological Chemistry Hoppe-Seyler 369 12751279
Meisel, H. & Frister, H. 1989 Chemical characterization of bioactive peptides from in vivo digests of casein Journal of Dairy Research 56, 343349
Migliore-Samour, D., Floc'H, F. & Jollés, P. 1989 Biologically active casein peptidcs implicated in immunomodulation. Journal of Dairy Research 56 357362
Migliore-Samour, D. & Jollés, P. 1988 Casein, a prohormone with an immunomodulating role for the newborn? Experientia 44 188193
Mykkänen, H. M. & Wasserman, R. H. 1980 Enhanced absorption of calcium by casein phosphopeptides in rachitic and normal chicks. Journal of Nutrition 110 21412148
Naito, H. & Suzuki, H. 1974 Further evidence for the formation in vivo of phosphopeptides in the intestinal lumen from dietary β-casein. Agricultural and Biological Chemistry 38 15431545
Pélissier, J. P. 1984 [Proteolysis of caseins.] Sciences des Aliments 4, 135
Petrilli, P., Picone, D., Caporale, G., Addeo, F., Auricchio, S. & Marino, G. 1984 Does casomorphin have a functional role? FEBS Letters 169 5356
Ribadeau-Dumas, B., Brignon, G., Grosclaude, F. & Mercier, J.-C. 1971 [Primary structure of bovine β-casein. Sequence of the C-terminal 65 amino acid residues.] European Journal of Biochemistry 20, 258263
Sato, R., Noguchi, T. & Naito, H. 1986 Casein phosphopeptide (CPP) enhances calcium absorption from the ligated segment of rat small intestine. Journal of Nutritional Science and Vitaminology 32 6776
Scanff, P., Savalle, B., Miranda, G., Pélissier, J.-P., Guilloteau, P. & Toullec, R. 1990 In vivo gastric digestion of milk proteins. Effect of technological treatments. Journal of Agricultural and Food Chemistry 38 16231629
Scanff, P., Yvon, M., Pélissier, J.-P., Guilloteau, P. & Toullec, R. 1991 Effect of some technological treatments of milk on amino acid composition of in vivo effluents during gastric digestion. Journal of Agricultural and Food Chemistry 39 14821487
Stan, E. Ya., Aleiˇnik, S. I. & Chernikov, M. P. 1983 [The physiologically active kappa-casein peptides.] Fiziologicheskiˇi Zhurnal SSSR im. I. M. Sechenova 69 855858
Svedberg, J., De Haas, J., Leimenstoll, K. G., Paul, F. & Teschemacher, H. 1985 Demonstration of β-casomorphin immunoreactive material in in vitro digests of bovine milk and in small intestine contents after bovine milk ingestion in adult humans. Peptides 6 825830
Tarr, G. E. 1982 In Methods in Protein Sequences Analysis, p. 223 (Ed. Elzinga, M.). Clifton, NJ: Humana Press
Umbach, M., Teschemacher, H., Praetorius, K., Kirshhäusser, R. & Bostedt, H. 1985 Demonstration of a β-casomorphin immunoreactive material in the plasma of newborn calves after milk intake. Regulatory Peptides 12 223230
Vasilkvskaya, L. S., Stan, E. Ya., Chernikov, M. P. & Shlygin, G. K. 1977 [Inhibiting effect of glycomacropeptide on gastric secretion caused by varions humoral stimulants.] Voprosy Pitaniya no. 4 2124
Visser, S. 1981 Proteolytic enzymes and their action on milk proteins. A review. Netherlands Mille and Dairy Journal 35 6588
Wybran, J., Appelboom, T., Famacy, J. P. & Govaerts, A. 1979 Suggestive evidence for receptors for morphine and methionine-enkephalin on normal human T lymphocytes. Journal of Immunology 123 10681070
Yvon, M. & Pélissier, J. P. 1987 Characterization and kinetics of evacuation of peptides resulting from casein hydrolysis in the stomach of the calf. Journal of Agricultural and Food Chemistry 35 148156

Characterization and kinetics of gastric emptying of peptides derived from milk proteins in the preruminant calf

  • P. Scanff (a1), M. Yvon (a1), S. Thirouin (a1) and J.-P. Pelissffir (a1)


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