Skip to main content Accessibility help
×
Home

Biological activity of camel milk casein following enzymatic digestion

  • Maryam Salami (a1), Ali Akbar Moosavi-Movahedi (a1) (a2), Faezeh Moosavi-Movahedi (a1), Mohammad Reza Ehsani (a3), Reza Yousefi (a4), Mohammad Farhadi (a5), Amir Niasari-Naslaji (a6), Ali Akbar Saboury (a1) (a2), Jean-Marc Chobert (a7) and Thomas Haertlé (a7)...

Abstract

The aim of this study was to investigate the effects of enzymatic hydrolysis with digestive enzymes of camel whole casein and beta-casein (β-CN) on their antioxidant and Angiotensin Converting Enzyme (ACE)-inhibitory properties. Peptides in each hydrolysate were fractionated with ultra-filtration membranes. The antioxidant activity was determined using a Trolox equivalent antioxidant capacity (TEAC) scale. After enzymatic hydrolysis, both antioxidant and ACE-inhibitory activities of camel whole casein and camel β-CN were enhanced. Camel whole casein and β-CN showed significant ACE-inhibitory activities after hydrolysis with pepsin alone and after pepsinolysis followed by trypsinolysis and chymotrypsinolysis. Camel β-CN showed high antioxidant activity after hydrolysis with chymotrypsin. The results of this study suggest that when camel milk is consumed and digested, the produced peptides start to act as natural antioxidants and ACE-inhibitors.

Copyright

Corresponding author

*For correspondence; e-mail: moosavi@ibb.ut.ac.ir

References

Hide All
Abd El-Salam, MH, El-Shibiny, S & Buchheim, W 1996 Characteristics and potential uses of the casein macropeptide. International Dairy Journal 6 327341
Abuja, PM & Albertini, R 2001 Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins. Clinica Chimica Acta 306 117
Agrawal, RP, Swami, SC, Beniwal, R, Kochar, DK, Sahani, MS, Tuteja, FC & Ghouri, SK 2003 Effect of camel milk on glycemic control, risk factors and diabetes quality of life in type-1 diabetes: a randomised prospective controlled study. Journal of Camel Practice and Research 10 4550
Aimutis, WR 2004 Bioactive properties of milk proteins with particular focus on anticariogenesis. Journal of Nutrition 134 S989S995
Arcan, I & Yemenicioğlu, A 2007 Antioxidant activity of protein extracts from heat-treated or thermally processed chickpeas and white beans. Food Chemistry 103 301312
Barzegar, A, Yousefi, R, Sharifzadeh, A, Dalgalarrondo, M, Chobert, JM, Ganjali, MR, Norouzi, P, Ehsani, MR, Niasari-Naslaji, A, Saboury, AA, Haertlé, T & Moosavi-Movahedi, AA 2008 Chaperone activities of bovine and camel β-caseins: importance of their surface hydrophobicity in protection against alcohol dehydrogenase aggregation. International Journal of Biological Macromolecules 42 392399
Cheung, HS, Wang, FL, Ondetti, MA, Sabo, EF & Cushman, DW 1980 Binding of peptide substrates and inhibitors of angiotensin-converting enzyme. Importance of the COOH-terminal dipeptide sequence. Journal of Biological Chemistry 255 401407
Clare, DA & Swaisgood, HE 2000 Bioactive milk peptides: a prospectus. Journal of Dairy Science 83 11871195
Collins, AR 2005 Antioxidant intervention as a route to cancer prevention. European Journal of Cancer 41 19231930
El-Hatmi, H, Girardet, JM, Gaillard, JL, Yahyaoui, MH & Attia, H 2007 Characterisation of whey proteins of camel (Camelus dromedarius) milk and colostrum. Small Ruminant Research 70 267271
Elias, RJ, Kellerby, SS & Decker, EA 2008 Antioxidant activity of proteins and peptides. Critical Reviews in Food Science and Nutrition 48 430441
Elias, RJ, McClements, DJ & Decker, EA 2005 Antioxidant activity of cysteine, tryptophan, and methionine residues in continuous phase β-lactoglobulin in oil-in-water emulsions. Journal of Agricultural and Food Chemistry 53 1024810253
Fiat, AM, Migliore-Samour, D, Jollès, P, Drouet, L, Sollier, CB & Caen, J 1993 Biologically active peptides from milk proteins with emphasis on two examples concerning antithrombotic and immunomodulating activities. Journal of Dairy Science 76 301310
Gómez-Ruiz, JA, Ramos, M & Recio, I 2004 Angiotensin converting enzyme-inhibitory activity of peptides isolated from Manchego cheese. Stability under simulated gastrointestinal digestion. International Dairy Journal 14 10751080
Halliwell, B 2001 Role of free radicals in the neurodegenerative diseases: therapeutic implications for antioxidant treatment. Drugs and Aging 18 685716
Harjanne, A 1984 Automated kinetic determination of angiotensin-converting enzyme in serum. Clinical Chemistry 30 901902
Holt, C 1997 The milk salts and their interaction with casein. In: Advanced Dairy Chemistry, 2nd Edition (Ed. Fox, PF). London, UK: Chapman & Hall
Huth, PJ, Layman, DK & Brown, PH 2004 The emerging role of dairy proteins and bioactive peptides in nutrition and health: foreword. Journal of Nutrition 134 961S
Imondi, AR & Stradley, RP 1974 Utilization of enzymatically hydrolyzed soybean protein and crystalline amino acid diets by rats with exocrine pancreatic insufficiency. Journal of Nutrition 104 793801
Jung, MY, Kim, SK & Kim, SY 1995 Riboflavin-sensitized photooxidation of ascorbic acid: kinetics and amino acid effects. Food Chemistry 53 397403
Kappeler, SR, Farah, Z & Puhan, Z 2003 5′-Flanking regions of camel milk genes are highly similar to homologue regions of other species and can be divided into two distinct groups. Journal of Dairy Science 86 498508
Kauf, ACW & Kensinger, RS 2002 Purification of porcine β-casein, N-terminal sequence, quantification in mastitic milk. Journal of Animal Science 80 18631870
Liu, Q, Raina, AK, Smith, MA, Sayre, LM & Perry, G 2003 Hydroxynonenal, toxic carbonyls, and Alzheimer disease. Molecular Aspects of Medicine 24 305313
López-Expósito, I, Quirós, A, Amigo, L & Recio, I 2007 Casein hydrolysates as a source of antimicrobial, antioxidant and antihypertensive peptides. Lait 87 241249
López-Fandiño, R, Otte, J & van Camp, J 2006 Physiological, chemical and technological aspects of milk-protein-derived peptides with antihypertensive and ACE-inhibitory activity. International Dairy Journal 16 12771293
Magjeed, NA 2005 Corrective effect of camel milk on some cancer biomarkers in blood of rats intoxicated with aflatoxin B1. Journal of the Saudi Chemical Society 9 253263
Marshall, T & Williams, KM 1993 Bradford protein assay and the transition from an insoluble to a soluble dye complex: effects of sodium dodecyl sulphate and other additives. Journal of Biochemical and Biophysical Methods 26 237240
Maruyama, S 1982 A peptide inhibitor of angiotensin I- converting enzyme in the tryptic hydrolysate of casein. Agricultural and Biological Chemistry 46 13931394
McLachlan, CNS 2001 β-casein A1, ischaemic heart disease mortality, and other illnesses. Medical Hypotheses 56 262272
Meisel, H 1998 Overview on milk protein-derived peptides. International Dairy Journal 8 363373
Meisel, H 2004 Multifunctional peptides encrypted in milk proteins. BioFactors 21 5561
Meisel, H 2005 Biochemical properties of peptides encrypted in bovine milk proteins. Current Medicinal Chemistry 12 19051919
Minervini, F, Algaron, F, Rizzello, CG, Fox, PF, Monnet, V & Gobbetti, M 2003 Angiotensin I-converting-enzyme-inhibitory and antibacterial peptides from Lactobacillus helveticus PR4 proteinase-hydrolyzed caseins of milk from six species. Applied and Environmental Microbiology 69 52975305
Okada, Y & Okada, M 1998 Scavenging effect of water soluble proteins in broad beans on free radicals and active oxygen species. Journal of Agricultural and Food Chemistry 46 401406
Otte, J, Shalaby, SM, Zakora, M, Pripp, AH & El-Shabrawy, SA 2007 Angiotensin-converting enzyme inhibitory activity of milk protein hydrolysates: effect of substrate, enzyme and time of hydrolysis. International Dairy Journal 17 488503
Pescuma, M, Hébert, EM, Rabesona, H, Drouet, M, Choiset, Y, Haertlé, T, Mozzi, F, Font de Valdez, G & Chobert, JM 2011 Proteolytic action/activity of Lactobacillus delbrueckii subsp. bulgaricus CRL 656 reduces antigenic response to bovine β-lactoglobulin. Food Chemistry 127 487492
Pihlanto-Leppälä, A, Koskinen, P, Phlola, K, Tupasela, T & Korhonen, H 2000 Angiotensin I-converting enzyme inhibitory properties of whey protein digests: concentration and characterization of active peptides. Journal of Dairy Research 67 5364
Rasmussen, LK, Due, HA & Petersen, TE 1995 Human αS1-casein: purification and characterization. Comparative Biochemistry and Physiology – B Biochemistry and Molecular Biology 111 7581
Re, R, Pellegrini, N, Proteggente, A, Pannala, A, Yang, M & Rice-Evans, C 1999 Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26 12311237
Rival, SG, Fornaroli, S, Boeriu, CG & Wichers, HJ 2001 Caseins and casein hydrolysates. 1. Lipoxygenase inhibitory properties. Journal of Agricultural and Food Chemistry 49 287294
Salami, M, Yousefi, R, Ehsani, MR, Dalgalarrondo, M, Chobert, J-M, Haertlé, T, Razavi, SH, Saboury, AA, Niasari-Naslaji, A & Moosavi-Movahedi, AA 2008 Kinetic characterization of hydrolysis of camel and bovine milk proteins by pancreatic enzymes. International Dairy Journal 18 10971102
Salami, M, Yousefi, R, Ehsani, MR, Razavi, SH, Chobert, J-M, Haertlé, T, Saboury, AA, Atri, MS, Niasari-Naslaji, A, Ahmad, F & Moosavi-Movahedi, AA 2009 Enzymatic digestion and antioxidant activity of the native and molten globule states of camel α-lactalbumin: possible significance for use in infant formula. International Dairy Journal 19 518523
Silk, DBA, Marrs, TC & Addison, JM 1973 Absorption of amino acids from an amino acid mixture simulating casein and a tryptic hydrolysate of casein in man. Clinical Science 45 715719
Tauzin, J, Miclo, L & Gaillard, J-L 2002 Angiotensin-I-converting enzyme inhibitory peptides from tryptic hydrolysate of bovine αS2-casein. FEBS Letters 531 369374
Tirelli, A, de Noni, I & Resmini, P 1997 Bioactive peptides in milk products. Italian Journal of Food Science 9 9198
Vermeirssen, V, Van Camp, J & Verstraete, W 2002 Optimisation and validation of an angiotensin-converting enzyme inhibition assay for the screening of bioactive peptides. Journal of Biochemical and Biophysical Methods 51 7587
Wong, PYY & Kitts, DD 2003 Chemistry of buttermilk solid antioxidant activity. Journal of Dairy Science 86 15411547

Keywords

Biological activity of camel milk casein following enzymatic digestion

  • Maryam Salami (a1), Ali Akbar Moosavi-Movahedi (a1) (a2), Faezeh Moosavi-Movahedi (a1), Mohammad Reza Ehsani (a3), Reza Yousefi (a4), Mohammad Farhadi (a5), Amir Niasari-Naslaji (a6), Ali Akbar Saboury (a1) (a2), Jean-Marc Chobert (a7) and Thomas Haertlé (a7)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed