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Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat

  • Pilar Gómez-Cortés (a1), Alfonso Cívico (a2), Miguel Angel de la Fuente (a1) and Andrés L. Martínez Marín (a2)

Abstract

This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis. In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively. Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d. In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant. Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil. Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns. The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.

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Corresponding author

Author for correspondence: Andrés L. Martínez Marín, Email: pa1martm@uco.es

References

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Keywords

Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat

  • Pilar Gómez-Cortés (a1), Alfonso Cívico (a2), Miguel Angel de la Fuente (a1) and Andrés L. Martínez Marín (a2)

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