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Survival and residual activity of Lactobacillus acidophilus frozen cultures under different conditions

Published online by Cambridge University Press:  01 June 2009

Roberto Foschino ,
Enrico Fiori  and
Antonietta Galli
Roberto Foschino
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione Microbiologia Agraria, Alimentare e Ecologica, Università degli Studi di Milano, via Celoria 2, I-20133 Milano, Italia
Enrico Fiori
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione Microbiologia Agraria, Alimentare e Ecologica, Università degli Studi di Milano, via Celoria 2, I-20133 Milano, Italia
Antonietta Galli
Affiliation:
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione Microbiologia Agraria, Alimentare e Ecologica, Università degli Studi di Milano, via Celoria 2, I-20133 Milano, Italia
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Summary

Eight strains of the Lactobacillus acidophilus complex were subjected, as concentrated cultures in skim milk, to different freezing treatments (rapidly in liquid nitrogen and more slowly in cold air at −30 °C), then stored at −80 and −30°C for 9 months. Survival was determined by plate counting with and without bile salts or sodium chloride at the highest tolerated concentration for each strain, to distinguish the undamaged population from the total population. Fermentative activity was measured as total lactic acid production by thawed cultures under standard conditions. Higher survival rates and greater activity were always obtained by storing cultures at −80 °C, but most strains stored at −30°C also survived well. Analysis of variance revealed that the viability of the frozen cultures was affected more by storage temperature than by cooling rate. Selective media were unable to distinguish the active population from the total surviving population. The correlation between values for activity and survival with selective media was poor.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 2 , May 1996 , pp. 295 - 303
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

REFERENCES

Boehringbr Mannheim Gmbh 1984 [Method of enzymatic food analysis: UV-Method for the determination of L-lactic and D-lactic acid in foodstuffs.] Milano: Boehringer Biochemia Robin SpAGoogle Scholar
Coppola, R. & Coppola, S. 1992 [Damage caused to thermophilic lactobacilli by low storage temperatures.] In Pre Atti del 24 Congresso Nazionale delta Società ItaMana di Microbiologia, Genova, pp. 95–96. Genova: Magazzini del CotoneGoogle Scholar
Davies, R. & Obafemi, A. 1985 Response of micro-organisms to freeze-thaw stress. In Microbiology of Frozen Foods, pp. 83–107 (Ed. Robinson, R. K.). London: Elsevier Applied Science PublishersGoogle Scholar
De Man, J. C, Rogosa, M. & Sharpe, M. E. 1960 A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23 130–135CrossRefGoogle Scholar
Foschino, R., Beretta, C. & Ottogalli, G. 1992 [A study of the best conditions for freezing and thawing lactic cultures. ]Industria del Latte 28(2) 49–67Google Scholar
Gilliland, S. E. & Lara, R. C. 1988 Influence of storage at freezing and subsequent refrigeration temperatures on β-galactosidase activity of Lactobacillus acidophilus. Applied and Environmental Microbiology 54 898–902CrossRefGoogle ScholarPubMed
International Commission On Microbiological Specifications For Foods 1980 Factors affecting life and death of microorganisms. 1. Temperature. In Microbial Ecology of Foods, vol. 1, pp. 1–37 (Eds Silliker, J. H., Elliott, R. P., Baird-Parker, A. C., Bryan, F. L., Christian, J. H. B., Clark, D. S., Olson, J. C. and Roberts, T. A.). London: Academic PressGoogle Scholar
Kandler, O. & Weiss, N. 1986 Regular, non-sporing Gram-positive rods. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1208–1234 (Eds Sneath, P. H. A., Mair, N. S., Sharpe, M. E. and Holt, J. G.). Baltimore, MD: Williams and WilkinsGoogle Scholar
Klaenhammer, T. R. & Kleeman, E. G. 1981 Growth characteristics, bile sensitivity and freeze damage in colonial variants of Lactobacillus acidophilus. Applied and Environmental Microbiology 41 1461–1467CrossRefGoogle ScholarPubMed
Mazuk, P. 1977 The role of intracellular freezing in the death of cells cooled at supraoptimal rates. Cryobiology 14 251–272Google Scholar
Salvi, F. & Chiandotto, B. 1978 [Statistical tables.] In Biometria: Principi eMelodi, pp. 528–529. Padova: Piccin EditoreGoogle Scholar
Smittle, R. B., Gilliland, S. E. & Speck, M. L. 1972 Death of Lactobacillus bulgaricus resulting from liquid nitrogen freezing. Applied Microbiology 24 551–554CrossRefGoogle ScholarPubMed
Tsvetkov, T. & Shishkova, I. 1982 Studies on the effects of low temperatures on lactic acid bacteria. Cryobiology 19 211–214CrossRefGoogle ScholarPubMed
Wright, C. T. & Klaenhammer, T. R. 1981 Calcium-induced alteration of cellular morphology affecting the resistance of Lactobacillus acidophilus to freezing. Applied and Environmental Microbiology 41 807–815CrossRefGoogle ScholarPubMed