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Prolactin-stimulated polymerization of acetyl-CoA carboxylase in explants of mid-pregnant rat mammary gland

Published online by Cambridge University Press:  15 October 2001

JULIUS E. OBEN  and
RAYMOND R. DILS
JULIUS E. OBEN
Affiliation:
School of Animal and Microbial Sciences, The University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, UK Present address: Medex Scientific UK Ltd, Horsham, West Sussex, RH12 1JE, UK.
RAYMOND R. DILS
Affiliation:
School of Animal and Microbial Sciences, The University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, UK
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Abstract

Prolactin significantly increased the rate of fatty acid synthesis in explants of mid-pregnant rat mammary gland cultured for 96 h with insulin plus corticosterone. Under these conditions, prolactin increased the specific activity of total acetyl-CoA carboxylase in nuclear-free homogenates of explants by 2·6, and increased the proportion of the enzyme in the active polymeric form from 0·44 to 0·89. Removal of prolactin after 48 h in culture decreased the specific activity of the total enzyme by about half, and decreased the proportion as polymer to 0·52. The results show that prolactin plays a major role in mid-pregnant rat mammary gland in the polymerization which accompanies increased activity of the total enzyme and increased rate of fatty acid synthesis.

Keywords

Acetyl-CoA carboxylasepolymerisationmammary gland
Type
Original article
Information
Journal of Dairy Research , Volume 68 , Issue 3 , August 2001 , pp. 351 - 355
Copyright
Proprietors of Journal of Dairy Research 2001

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