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The prevalence, molecular characterization and antimicrobial resistance profiling of Streptococcus agalactiae isolated from clinical mastitis cases on large dairy farms in China

Published online by Cambridge University Press:  28 February 2022

Kai Liu
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, People's Republic of China
Limei Zhang
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, People's Republic of China
Xiaolong Gu
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, People's Republic of China
Gang Liu
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China
Yang Liu
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China
Peng Chen
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China
Zhaoju Deng
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China
Jian Gao
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China
Bo Han
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China
Weijie Qu*
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, People's Republic of China
*
Author for correspondence: Weijie Qu, Email: 1401075010@qq.com

Abstract

This research communication aims to characterize the prevalence, molecular characterization and antimicrobial resistance profiling of Streptococcus agalactiae isolated from clinical mastitis in China. A total of 140 Strep. agalactiae isolates were identified from 12 out of 201 farms in 6 provinces, overall herd prevalence was 18.6% and the MLST analysis showed clonal complexes (CC) 103 and CC 67 were present in these herds with CC 103 predominant, accounting for 97.9%. Isolates were mostly sensitive to the tested antimicrobials: penicillin, ceftiofur, amoxi/clav, cefquinome, and vancomycin (100%), followed by cefalexin (97.9%), oxacillin (96.4%), enrofloxacin (95.7%), erythromycin (89.3%), and clindamycin (88.6%). Only 19.3 and 0.7% of isolates were sensitive to tetracycline and daptomycin, respectively, and sequence type (ST) 103 was most resistant to antimicrobials. In conclusion, CC 103 was the predominant subgroup of bovine mastitis Strep. agalactiae in China, and most antimicrobials apart from tetracycline and daptomycin were effective.

Type
Research Article
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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