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DNA fingerprinting of thermophilic lactic acid bacteria using repetitive sequence-based polymerase chain reaction

Published online by Cambridge University Press:  19 October 2000

PATRICIO J. DE URRAZA
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas, UNLP-CONICET, La Plata 1900, Argentina Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, Argentina
ANDREA GÓMEZ-ZAVAGLIA
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas, UNLP-CONICET, La Plata 1900, Argentina
MARIO E. LOZANO
Affiliation:
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, Argentina
VICTOR ROMANOWSKI
Affiliation:
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, Argentina Instituto de Bioquímica y Biología Molecular (IBBM), Facultad de Ciencias Exactas, UNLP, La Plata 1900, Argentina
GRACIELA L. DE ANTONI
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas, UNLP-CONICET, La Plata 1900, Argentina Comisión de Investigaciones Científicas y Tecnológicas de la Provincia de Buenos Aires, La Plata 1900, Argentina

Abstract

DNA fingerprints of lactic acid bacteria were generated by polymerase chain reaction using a primer based on the repetitive elements found in the genome of Streptococcus pneumoniae (BOX-PCR). The method made it possible to identify 37 isolates from raw milk, industrial starters and yogurt. Differentiation at species, subspecies and strain level was possible for Lactobacillus delbrueckii subsp. lactis, Lb. delbrueckii subsp bulgaricus and Str. thermophilus. BOX-PCR was also applied to studying the strain composition of a starter culture and the direct detection of strains in commercial fermented milk.

Type
Research Article
Copyright
Proprietors of Journal of Dairy Research 2000

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