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Association of SNP and STR polymorphisms of insulin-like growth factor 2 receptor (IGF2R) gene with milk traits in Holstein-Friesian cows

Published online by Cambridge University Press:  22 May 2018

Marta Dux ,
Magdalena Muranowicz ,
Eulalia Siadkowska ,
Dagmara Robakowska-Hyżorek ,
Krzysztof Flisikowski ,
Emilia Bagnicka  and
Lech Zwierzchowski
Marta Dux
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Magdalena Muranowicz
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Eulalia Siadkowska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Dagmara Robakowska-Hyżorek
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Krzysztof Flisikowski
Affiliation:
Chair of Livestock Biotechnology, Technical University of Munich, Liesel-Beckmannstr.1, D-85354 Freising, Germany
Emilia Bagnicka
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Lech Zwierzchowski*
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
*
*For correspondence; e-mail: l.zwierzchowski@ighz.pl
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Abstract

The objective of the study reported in this Research Communication was to investigate the association of polymorphisms in the insulin-like growth factor receptor 2 (IGF2R) gene with milk traits in 283 Polish Holstein-Friesian (PHF) cows from the IGAB PAS farm in Jastrzębiec. IGF2R regulates the availability of biologically active IGF2 which is considered as a genetic marker for milk or meat production in farm animals. Two novel genetic polymorphisms were identified in the bovine IGF2R gene: a polymorphic TG-repeat in intron 23 (g.72389 (TG)15-67), and a g.72479 G > A SNP RFLP-StyI in exon 24. The following milk traits were investigated: milk yield, protein and fat yield, SCC and lactose content. To determine the influence of the IGF2R STR and SNP genotypes on the milk traits, we used the AI-REML (average information restricted maximum likelihood) method with repeatability, multi-trait animal model based on test-day information using DMU package. Statistical analysis revealed that the G/A genotype (P ≤ 0·01) was associated with milk and protein yield, lactose content and somatic cell count (SCC) in Polish HF cows. TGn (29/22, 28/29, 28/22, 28/28) genotypes were associated with high values for milk, (28/22, 28/23) with protein and fat yield, (25/20) with lactose content, and (29/33, 28/28) with low SCC. We suggest that the IGF2R gene polymorphisms could be useful genetic markers for dairy production traits in cattle.

Keywords

TGn microsatelliteA/G transitiongenetic markerassociationdairy traits
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 138 - 141
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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References

Abu El-Magd, M, Abo-Al-Ela, HG, El-Nahas, A, Saleh, AA & Mansour, AA 2014 Effects of a novel SNP of IGF2R gene on growth traits and expression rate of IGF2R and IGF2 genes in gluteus medius muscle of Egyptian Buffalo. Gene 540 133–139CrossRefGoogle Scholar
Bagnicka, E, Siadkowska, E, Strzałkowska, N, Żelazowska, B, Flisikowski, K, Krzyżewski, J & Zwierzchowski, L 2010 Association of polymorphisms in exons 2 and 10 of the insulin-like growth factor 2 (IGF2) gene with milk production traits in Polish Holstein-Friesian cattle. Journal of Dairy Research 77 37–42CrossRefGoogle ScholarPubMed
Bagnicka, E, Strzałkowska, N, Flisikowski, K, Szreder, T, Jóźwik, A, Prusak, B, Krzyżewski, J & Zwierzchowski, L 2007 The polymorphism in the β 4-defensin gene and its association with dairy production traits and somatic cell count in Holstein-Friesian cows. Journal of Animal Breeding and Genetics 124 150–156CrossRefGoogle ScholarPubMed
Berkowicz, EW, Magee, DA, Berry, DP, Sikora, KM, Howard, DJ, Mullen, MP, Evans, RD, Spillane, C & MacHugh, DE 2012 Single nucleotide polymorphisms in the imprinted bovine insulin-like growth factor 2 receptor gene (Igf2r) are associated with body size traits in Irish Holstein-Friesian cattle. Animal Genetics 43 81–87CrossRefGoogle ScholarPubMed
Berkowicz, EW, Magee, DA, Sikora, KM, Berry, DP, Howard, DJ, Mullen, MP, Evans, RD, Spillane, C & MacHugh, DE 2011 Single nucleotide polymorphisms at the imprinted bovine insulin-like growth factor 2 (IGF2) locus are associated with dairy performance in Irish Holstein-Friesian cattle. Journal of Dairy Research 78 1–8CrossRefGoogle ScholarPubMed
Brotherstone, S, White, IMS & Meyer, K 2000. Genetic modelling of daily milk yield using orthogonal polynomials and parametric curves. Animal Science 70 407–415CrossRefGoogle Scholar
Dehoff, MH, Elgin, RG, Collier, RJ & Clemmons, DR 1998 Both type I and II insulin-like growth factor receptor binding increase during lactogenesis in bovine mammary tissue. Endocrinology 122 2412–2417CrossRefGoogle Scholar
Dunner, S, Sevane, N, Garcia, D, Leveziel, H, Williams, JL, Mangin, B & Valentini, A 2013. Genes involved in muscle lipid composition in 15 European Bos Taurus breeds. Animal Genetics 44 493–501CrossRefGoogle ScholarPubMed
Flisikowski, K, Adamowicz, T, Strabel, T, Jankowski, T, Switoński, M & Zwierzchowski, L 2007 An InDel polymorphism in exon 6 of IGF2 is associated with the breeding value of Polish Holstein-Friesian bulls. Biochemical Genetics 45 139–143CrossRefGoogle ScholarPubMed
Kornfeld, S 1992 Structure and function of the mannose 6 phosphate/insulin-like growth factor II receptors. Annual Review of Biochemistry 61 307–330CrossRefGoogle Scholar
Madsen, P & Jensen, J 2000 A user's guide to DMU: A package for analysis multivariate mixed models. Version 6. Release 4. Foulum, Denmark: National Institute of Animal Science, DJFGoogle Scholar
Maj, A, Korczak, M, Bagnicka, E, Zwierzchowski, L & Pierzchała, M 2007 A TG-repeat polymorphism in the 5′-noncoding region of the goat growth hormone receptor gene and search for its association with milk production traits. Small Ruminant Research 67 279–284CrossRefGoogle Scholar
Ron, M, Israeli, G, Seroussi, E, Weller, JI, Gregg, JP, Shani, M & Medrano, JF 2007 Combining mouse mammary gland gene expression and comparative mapping for the identification of candidate genes for QTL of milk production traits in cattle. BMC Genomics 20 183–194CrossRefGoogle Scholar
Rzewuska, K & Strabel, T 2013 Genetic parameters for milk urea concentration and milk traits in Polish Holstein-Friesian cows. Journal of Applied Genetics 54 473–482CrossRefGoogle ScholarPubMed
Smaragdov, MG, Prinzenberg, EM & Zwierzchowski, L 2006 QTL mapping at cattle: theoretical and empirical approach. Animal Science Papers and Reports 24 69–110Google Scholar
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