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632. Reactivation of milk phosphatase following heat treatment: IV. The influence of certain metallic ions

Published online by Cambridge University Press:  01 June 2009

R. C. Wright  and
J. Tramer
R. C. Wright
Affiliation:
United Dairies Research Laboratories, Wood Lane, London, W. 12
J. Tramer
Affiliation:
United Dairies Research Laboratories, Wood Lane, London, W. 12
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Extract

1. Reactivation of alkaline milk phosphatase is affected by the presence of ions, Mg2+, Zn2+ and Mn2+ being activating and Cu2+, Ni2+ and Co2+ being inhibitory.

2. Cu2+ will inhibit reactivation induced by Mg2+, but has no effect upon Zn2+-induced reactivation.

3. Using washed cream, either alone or in admixture with boiled whey treated with cationic resin, reactivation occurs in the presence of added Mg2+, but not when Zn2+ is added.

4. Experiments with EDTA. confirm that metallic ions play a part both in alkaline phosphatase activity and in reactivation.

5. It is suggested that either Mg2+ or Zn2+ or both play a part in reactivation.

Type
Original Articles
Information
Journal of Dairy Research , Volume 23 , Issue 2 , June 1956 , pp. 248 - 256
Copyright
Copyright © Proprietors of Journal of Dairy Research 1956

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References

REFERENCES

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