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31P-nuclear magnetic resonance study of milk fractions

  • Magnus Wahlgren (a1), Torbjörn Drakenberg (a2), Hans J. Vogel (a2) and Petr Dejmek (a1)


Milk serum, whey and milk ultrafiltrate were examined by 31P nuclear magnetic resonance (31P-NMR). About 20 phosphorylated milk constituents gave rise to resonances in the spectra. Most of these have been assigned to such well-known milk constituents as inorganic phosphate, N-acetylglucosamine-1-phosphate and glycerophosphorylcholine. Resonances from previously unknown constituents such as phosphocreatine were also observed. When the pH-dependence of inorganic phosphate, N-acetylglucosamine-1 -phosphate, glycerophosphorylcholine and gly-cerophosphorylethanolamine was examined it was observed that the resonance of inorganic phosphate overlapped that of N-acetylglucosamine-1-phosphate around neutral pH. This is the most probable explanation as to why this constituent was not observed in earlier 31P-NMR studies on milk.



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31P-nuclear magnetic resonance study of milk fractions

  • Magnus Wahlgren (a1), Torbjörn Drakenberg (a2), Hans J. Vogel (a2) and Petr Dejmek (a1)


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