Thin-layer chromatography and infra-red spectrosoopy were used to identify the components of technical Thanite. A method is developed for qualitative and quantitative determination using a gas liquid chromatograph (GLC) equipped with a flame ionization detector. Two major peaks, having retention times 1 and 5 min, were obtained. This was achieved using a glass column (15 × 0·3 cm), packed with diethylene glycol (1·5%) on chromosorb W, 60–80 mesh, at temperature range 70–170 °C. The results obtained by GLC showed that 33·7–55·8%, and 36·6–68·3% of the initial deposit of Thanite on cotton leaves degraded within 12 h from exposure to sunlight and ultraviolet irradiation respectively. One fifth of the initial deposit of Thanite on cotton leaves was lost after 24 h from spraying, then 70 % of the compound was dissipated after 3 days. Incubation of Thanite with rumen liquor at 39 °C for 24 h under anaerobic conditions resulted in degradation of 45–74% of the initial concentration.

Corriedale and Dorset Horn castrate male (wether) lambs reared at pasture were weaned at 19 kg live weight (LW), brought indoors and fed a high protein diet such that half of each group grew at ca. 200 g/day and the other half at ca. 100 g/day. Animals were slaughtered at 25 and 30 kg LW and chemical composition (protein, fat, energy, water and ash) of the body determined. The relationship between each body component and shorn empty-body weight was examined by regression analysis.

Within levels of feeding the results were similar in the two breeds. When comparisons were made between feeding levels, the slower-growing animals contained more fat, energy and ash than the faster-growing group, less water, but similar amounts of protein at any given empty-body weight.

Although salt injury in plants is due to both osmotic and specific ion effects, little is known about the reaction of rice plants to salinity under increasing sodium adsorption ratios (SAR) in the growth medium. Therefore, a glasshouse experiment was conducted to study the effect of four levels of salt concentration (20, 40, 60 and 80 m-equiv/1 with respect to the sum of Na+, Ca2+ and Mg2+) and four levels of SAR (2, 25, 50 and 75) on growth and mineral composition of rice cvs IR 2153-26-3 and IR 26. The results show that as salt concentration increased, dry-matter yield and percentage water content of shoots decreased regardless of the variety. The rate of decline with salinity was most pronounced under low SAR and in the salt-sensitive variety IR 26. With increase in SAR, the yield, percentage water content and concentrations of Ca, Mg, and K in shoots and roots decreased, but the effects were more marked at the lowest salt concentration and in IR 26. Variety IR 2153-26-3 showed greater tolerance, maintaining a higher concentration of Ca and higher Ca/Na and K/Na ratios and a lower concentration of Na and Cl in the tops compared with IR 26.

The degree of salt injury in rice seems to depend not only on salt concentration but also on SAR in the growth medium whilst salt tolerance is associated with tolerance for both high salt concentration and high SAR. Therefore, in studies on screening rice varieties for salt and sodium tolerance, the interactive effects of SAR and salinity must be considered so as to ensure greater adaptability by the varieties recommended for salt-affected soils.

Forty-eight 4-month-old lambs, reared worm-free from birth, were allocated to six groups and offered ad libitum a complete diet containing 139 g crude protein/kg D.M. One group was killed at the outset as initial control (CI). Further groups, ALC, 1, 2, 3 and 4 were dosed daily for 84 days with zero, 1000, 3000, 5000 and 5000 larvae of Ostertagia circumcincta, respectively. Lambs in group 4 also received anthelmintic (fenbendazole, 5 mg/kg) on days 21, 42, 63 and 84. Lambs were killed after 98 days and their carcasses and those of the initial controls analysed for fat, protein and mineral contents.

The average serum T4(D) of nine non-pregnant buffaloes was 5·5 ± 1·4 μg/100 ml. It increased slightly to 6·2 ± 4·2 μg/100 ml in 37 6–7 month pregnant buffaloes. Nevertheless the difference was not significant. When the pregnancy reached 8–9 months, the T4(D) was 9·0 ± 3.8μg/100 ml which was significantly higher than that at 6–7 months (P < 0·01). At full term (9–10 months), T4(D) decreased to 3·6 ± 2–6 μg/100 ml which was significantly lower than that at 8–9 months (P <0·001).

In 1 month post-partum buffaloes, the average T4(D) was 5·1 ± 3·4 μg/100 ml, while T4(D) of 1-month-old buffalo calves was very high, i.e. 13·6 ± 3·2 μg/100 ml.

The total serum T3 (RIA) of late-pregnant buffaloes decreased markedly from that of 8–9 month pregnant (P< 0·001). At 1 month post-partum, T3 rose to 143·4 ± 33·0 μg/100 ml which was significantly higher than that at late pregnancy (P < 0·001). The mean serum T3(RIA) of 1-month-old buffalo calves was relatively high, i.e. 281·0 ± 106·2 μg/100 ml which was significantly higher than 143·4 ± 33·0 ng/100 ml of their darn buffaloes (P < 0·001) at the same period of time.

The RT3U values of 6–7 (35·9 ± 4·2%) and 8–9 (34·7 ± 2·0%) month pregnant animals were highly significantly different from 31·1 ± 3·1 % of the full-term pregnancy (P < 0·001).

Maize was grown for grain or forage on the same site at Woburn for 7 consecutive years. Yields were satisfactory and there were no serious pest or disease problems, provided the small plots were netted until young plants were established. Different rates of nitrogen fertilizer were tested with and without annual applications of a soil sterilant, dazomet. Average yields of grain (85% dry matter) and forage (dry matter) were respectively 4·5 and 8·8 t/ha with 50 kg N/ha and 5·0 and 9·5 t/ha with 100 kg N/ha. Without dazomet there was a further increase with 150 kg N/ha. Weather influenced the response to nitrogen, and the response to a split dressing of the highest rate was variable. Except for plots receiving the split dressing of N in 1973 and 1974 dazomet always increased yields: grain yields were increased on average by 1 t/ha and forage by 1·6 t/ha. Dazomet-treated plots had taller and heavier plants with more stems, leaves and nodal roots, but fewer seminal roots than untreated plots. Dazomet-treated plots also had fewer migratory nematodes and smaller and less varied root myoofloras. Most of these differences were greatest in the young crop and possible reasons for this ‘early boost’ are discussed.

Different seeding combinations and monocultures of sorghum forage, Abu Sabein, and legume forage, Lubia, were grown under irrigation on saline marginal lands for two seasons. Certain seeding combination treatinents, particularly (¾ Abu Sabein + ¼ Lubia), produced higher total D.M. than the higher yielding component, Abu Sabein, grown as a monoculture. This mixture treatment, together with some others, yielded more total crude protein than Abu Sabein alone. The seeding combination treatments influenced the pattern of D.M. distribution amongst different strata in the crop canopy. As the percentage of Lubia in the mixture increased, the D.M. of Abu Sabein tended to accumulate in greater proportions in the higher strata.

A strain of Rhizobium isolated from the root nodule of lentil was caused to produce mutants and screened on a medium of pH 4·5. Five mutant colonies of Rhizobium were isolated from lentil which differed in growth over a pH range 4·5–7·5. Strains differed in their growth in acidic and calcareous soils and also in their effectiveness of N fixation.

Wheat (Triticum aestivum L.) cv. Gamenya was grown in a Mediterranean type environment in two separate field experiments, to determine the effects of nitrogen application and date of seeding on floret survival. One treatment, intermediate in nitrogen level and sowing date, was common in both experiments. Rates and durations of spikelet initiation and durations of the floret initiation period were estimated. At maturity, yield and yield components were measured and occurrence of individual grains at each floret position on odd-numbered spikelets were recorded. The time of floret initiation relative to terminal spikelet formation and the subsequent occurrence of grain were examined.

Florets developed first in spikelets 5-13 and last in spikelet 1. Added nitrogen hastened the initiation of florets but did not affect the number initiated; it also increased the numbers of grains per spikelet. In the last sowing date treatment, time to floret initiation decreased, but the sequence of floret initiation was unaffected.

Numbers of florets and grains on tillers were less than on main shoots. Only about 28% of all florets initiated developed into grain.

The main conclusion was that the florets which initiated before the terminal spikelet formed grain and those that initiated afterwards did not; we accordingly postulate that the vascular system between the rachis and the floret develops as the floret is initiated, but that after terminal spikelet formation, this is inhibited and further grains then fail to develop.

The weights of components of the alimentary tract were recorded in 55 swamp buffaloes and 51 zebu cattle slaughtered at Bogor in West Java. These were related to hot carcass weight (HCW) and slaughter live weight (SLW), the latter being calculated from locally developed regressions relating live weight to chest girth. Huxley's (1932) allometric equation was used in its logarithmic form as the basis for covariance analyses of the data. A stepwise regression analysis was also undertaken using a linear model relating HCW and gut component weight to species, sex, age and slaughter condition with SLW as the independent variable.

There were significant differences in dressing percentage between buffaloes (38·1 %) and zebus (44·8%) at 340 kg SLW and these were due to species differences in full gut weight rather than weights of other non-carcass components. The allometric growth coefficients for total gut, or gut component, were generally higher in buffaloes than zebus, but the differences were not statistically significant. Together with other published data, these observations suggest that the gut contents may contribute more to the increase in live weight of buffaloes than in zebus.

At the same SLW or HCW, the buffaloes had the heavier weights of full and empty total gut, full and empty reticulo-rumen and total gut contents. At the same total empty gut weight, buffaloes had the heavier empty reticulo-rumen. It was suggested that Indonesian swamp buffaloes and zebu cattle fed on village-type low-quality diets may also differ in the rate of development of their alimentary tract and this could have implications for their comparative nutritional physiology.

The effects of plant population density, defoliation and removal of pods and apical buds on dry matter, nodulation and nitrogen accumulation in field bean (Viciafaba L.) were investigated in the field in 1973 and 1974.

In 1973 seed yield, dry matter and nitrogen accumulation were similar at 50 and 150 plants/m2 while in 1974 40 plants/m2 was significantly different from 80, 120 and 160 plants/m2 which were similar in these attributes. At similar densities, these crops generally had similar leaf areas and nodule weights/m2 during the early pod-filling period while at earlier growth stages leaf area and nodule development were greater at the higher plant population densities. Leaf area and nodule weight per plant were positively correlated as were absolute growth rate per plant and nitrogen assimilation rate per unit weight of nodule.

Substantial leaf removal reduced weight but not the nitrogen assimilation rate of nodules determined from plant nitrogen content. Leaf removal did not significantly reduce dry matter but reduced nitrogen yield by reducing seed yield provided that the removed leaves were accounted for.

Pod removal reduced dry-matter and nitrogen yield as well as nitrogen assimilation rate of nodules. Pod removal, however, substantially increased number and weight of nodules. Most of these late-formed nodules were located on the older parts of the lateral roots. Removal of the shoot apex had minor effects on nitrogen yield but reduced nodulation towards the completion of pod-filling.

Hereford heifers were fed a diet (7·0 MJ ME/kg D.M.) containing 0·09 % phosphorus but complete with regard to other nutrient constituents. Only after 19 weeks wore the effects of subclinical aphosphorosis evident in the form of reduction in feed intake, cessation of weight gain, increased feed conversion and decreases in tho concentration of inorganic phosphorus in blood and saliva. Heifers supplemented daily with 12 g phosphorus sustained a weight gain of about 0–2 kg/day over 62 weeks as did other supplemented heifers whose feed intake was restricted to that of tho low phosphorus treatment. The effects of phosphorus supplementation were thus demonstrated without being confounded by concomitant increases in feed intake.

Phosphorus supplementation resulted in significantly lowered apparent crude protein digestibility and a slight increaso in cell wall digestibility.

The carcass composition of phosphorus-deficient heifers of lower body weight was similar to ad libitumand restricted-fed controls supplemented daily with an extra 12 g phosphorus. The skeleton of heifers fed a phosphorus deficient diet for 63 weeks contained 18·8% less ash than restricted-fed controls given an extra 12 g phosphorus daily, but not less than ad libitum fed controls. Supplements of phosphorus and energy had different effects on the weights and concentration of bone minerals in skeletal components. A phosphorus supplement increased deposition of phosphorus in head, feet and tail, limbs and vertebrae and increased phosphorus concentration in head, feet and tail, vertebrae, ribs 1–5 and ribs 10–13. Extra energy intake slightly increased phosphorus accretion only in ribs 6–9 but had no effect on its concentration in this and other skeletal components. Only one (40 weeks) of the six biopsy samples taken from the cortex of the 11th and 12th ribs during the course of the experiment reflected the effect of uncomplicated phosphorus deficiency. No treatment differences were found in biopsy samples taken at slaughter in spite of differences in rib 10–13 analyses. The implications of this are discussed as they relate to the use of this method to monitor the phosphorus status of cattle.

In a comparative study of some factors that influence the body weights of 36 Friesian, 54 Holstein, 22 Holstein-Friesian, 24 Jersey and 17 Brown Swiss calves born during 1964–9 at the Moor Plantation dairy herd, Ibadan, breeds differed significantly (P <0·01) at all ages. Highly significant sex differences (P <0·01) existed at 4 and 8 weeks of age. The season of birth significantly influenced the weight at 4 and 8 (P < 0·05) and at 12 weeks (P < 0·01). The season × sex and season × breed interactions significantly affected the weights at virtually all ages but the sex × breed interaction did not. The birth weight exerted a highly significant influence (P < 0·01) on all other body weights. It reduced the residual variation as well as the differences and interactions between the main effects. After adjusting for birth-weight differences, body weight was significantly (P < 0·05) influenced at various ages by breed of calf, season of birth, sex × breed and season × breed interactions. The sex and season × sex interaction effects on adjusted body weights at all ages were negligible (P > 0·05).

The energy and water balance for Bedouin goats before gestation and at different levels of production was worked out. Both gross energy and total water intake were linearly related to their milk production. High producing animals consumed twice as much food (up to 1256kJ/kg body weight/day) and three times more water (290ml/kg body weight/day) as non-lactating ones.

The conversion coefficient of digestible energy to energy in milk ranged from 34% at peak lactation to 25% at the end of the period. No mobilization of tissue was observed even at peak lactation. Black Bedouin goats thus demonstrate ‘superior dairy merits’ throughout their entire lactating period.

Water requirement of a lactating Bedouin goat was lower than reported for other ruminants and amounted to 2·5 l/kg milk produced. This ratio was maintained whether over 2 l of milk was produced or only 0·5 1.

Compared with goats before gestation, lactating Bedouin goats ‘economize’ both their energy and water metabolism and were highly adapted to maintain production in the adverse desert environment.

Mature Merino ewes (n = 320) were fed either maintenance (100% M) or 25% maintenance (25% M) rations from the day after mating until embryos were collected at either day 11 or 21 after mating. On the 4th day after mating 223 ewes were ovariectomized and given 5, 10, 15, 20 or 25 mg progesterone/day (intramuscular) until embryo collection. A further group of sham operated ewes (n = 54) acted as controls. Despite live-weight losses of 10·7 and 15·8% from mating to days 11 and 21 after mating respectively, ewes on 25% M rations showed no evidence that embryonic survival was influenced by undernutrition. The embryos from ewes fed 25 % M rations were significantly (P < 0·05) smaller than those fed 100% M (crown-rump measurements were 5·93 ± 0·139 mm and 6·39 ± 0·116 mm at day 21 after mating in the 25 % M and 100% M groups respectively). Development, assessed by formation of the first pharangeal lobe and the fore- and hind-limb buds, was significantly retarded (P < 0·05) in the 21-day-old embryos from the undernourished ewes. Embryo survival in ovariectomized ewes receiving 5 or 10 mg progesterone/day was significantly (P < 0·05) lower than in ovariectomized ewes receiving 20 and 25 mg progesterone/ day at day 11 after mating. Embryo survival at both days 11 and 21 after mating increased with increasing doses of progesterone. Ewes fed 25% M rations had elevated (P< 0·001) concentrations of peripheral progesterone compared with those fed 100% M. This occurred when the progesterone was derived from either exogenous or endogenous (ovarian) sources, dismissing the possibility that changes in secretion rates could account for these observed differences. Differences in embryo growth and development were not related to these variations in the plasma progesterone concentration. Undernutrition restricted both growth and development to 21 days of pregnancy.

Experiments were made on a sandy clay loam soil with five crops to determine the effects of thorough loosening of the subsoil and deep incorporation of nutrients on yields during the subsequent 4 years.

Loosening to 0·9 m increased fresh-weight yields by between nil and 95% depending on the crop and season. Responses were still considerable 4 years after the initial loosening and there was no evidence of any decline with time. Deep incorporation of nutrients did not improve yields.

Loosening gave a durable increase in the volume of coarse pores and decreases in bulk density and penetrometer resistance. It also nearly doubled the rate of root extension when estimates were made for one crop, consistently increased water extraction from below 30 cm and, on occasion, reduced plant water stress.

It is concluded that most of the benefits from deep loosening resulted from improvements i the rate at which plants could extract water rather than nutrients from the subsoil.

Experiments were made between 1977 and 1979 to investigate the possibility of raising potato yields by mixing together cultivars having different growth patterns. The experiments also included mixtures of unsprouted and sprouted seed of the same or different cultivars. In the first experiment (1977) Pentland Crown was mixed with Arran Pilot, Red Craig's Royal or Désirée and the binary mixtures (1:1) compared with the pure stands of each. Yield of tubers from the mixtures did not differ significantly from the mean of two components planted alone at wide or narrow spacing of the seed tubers. Two experiments with identical treatments (1978) included mixtures of Majestic and Désirée and of each cultivar with unsprouted and sprouted seed. Mixtures (1:1) yielded more tubers than the mean of the two components planted alone and at final harvest in one experiment unsprouted and sprouted Majesties gave 7·6 t/ha more than the mean of these and 6·9 t/ha more than the more productive culture (P < 0·05). Similar differences were obtained in the second experiment.

In the final experiment (1979) the degree of sprout development at planting time was varied in the cultivar Majestic. Short or long sprouted seed was mixed with unsprouted seed and long sprouted Désirée was also mixed with unsprouted Majestic. Mixtures of the unsprouted cultivar with either long sprouted Majestic or Désiré exceeded, significantly, the mean yield of components planted alone by 4·1 and 5·8 t/ha, respectively, at a high level of fertilizer. As a mean of three experiments the mixture of sprouted and unsprouted Majesties yielded 6·26 t/ha more tubers than the mean of the components and 4·27 t/ha more than the higher yielding component in pure culture. Better light interception due to higher leaf area duration and a more persistent leaf canopy appeared to produce these effects.

The yield and chemical composition of Sabrina hybrid ryegrass and RvP Italian ryegrass were compared, with special reference to regrowth after defoliation in spring and early summer. Initial cuts were taken on 6, 20 April and 4. and 18 May; subsequently a conservation crop was harvested at either 5 or 7 weeks, and a second regrowth 30 days later. There were four fertilizer treatments. Tiller density, stem development and individual tiller dry weight were recorded in selected treatments.

At the initial cuts and in the conservation crops after the first three initial cuts, varietal differences in yield and dry-matter digestibility (DMD) were relatively small. In all other harvests, RvP was appreciably higher yielding than Sabrina, and it showed greater responses to fertilizer in all second regrowth harvests. The water-soluble carbohydrate (WSC) content of RvP was consistently higher than that of Sabrina in regrowth harvests; the DMD was higher in Sabrina.

In primary growth of the two cultivars, tillering was suppressed at the time of head emergence, but active tillering was resumed earlier in RvP, the later-heading cultivar. In RvP, tillers arising early in the regrowth periods attained head emergence within 5 weeks. In Sabrina there was a marked decline in head production during June, and an increase in the size of individual vegetative tillers.

In regrowth harvests both the yield and the % WSC were markedly reduced when drought conditions were most severe; the most marked varietal differences occurred at this time, both in terms of yield of dry matter and % crude protein responses to fertilizer treatment.

In the two cultivars the DMD of 7-week conservation crops harvested in late May was at least equal to that of 5-week crops harvested later during drought conditions. A distinctly more advanced stage of development was attained in the earlier, heavieryielding crops.

Substrate and nitrogen metabolism of ruminant muscle was studied in vitro using a perfused sheep hemidiaphragm preparation. Glucose, acetate, propionate and butyrate were taken up by the perfused muscle at a constant rate over 2 h. Rate of acetoacetate uptake declined over the perfusion period. Net production of 3-hydroxybutyrate over the perfusion period was observed.

Release of alanine and glycine by the perfused muscle could not be accounted for by protein breakdown, implicating muscle as a site of synthesis of these amino acids. The perfused muscle metabolized aspartate and arginine to a considerable extent, but the branched-chain amino acids only to a small extent. Only a small proportion, approximately 5%, of the alanine produced by the perfused muscle was derived from glucose.

In the presence of amino acids, at twice the concentration found in plasma, and insulin, some amino acids showed a net uptake by the diaphragm but when cysteine was omitted from the perfusate, all amino acids with the exception of arginine showed a net output. Evidence was obtained that protein synthesis was reduced in the absence of cysteine. The output of Nτ methylhistidine by the preparation was much higher than could be accounted for by estimates of protein breakdown using isotope dilution techniques. These data are, however, consistent with the view that Nτ methylhistidine is not a good index of muscle protein breakdown in sheep.