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Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

  • W. Li (a1) (a2), H. Gong (a2) (a3), H. Zhou (a1) (a2) (a3), J. Wang (a1) (a2), X. Liu (a1) (a2), S. Li (a1) (a2), Y. Luo (a1) (a2) and J. G. H. Hickford (a2) (a3)...

Abstract

Keratin-associated proteins (KAPs) are constituents of wool and hair fibres and are believed to play an important role in determining the characteristics of the fibres. In the current study, a polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) approach was used to screen for variation in the ovine KAP15-1 gene (KRTAP15-1). Four PCR-SSCP banding patterns, representing four different variants (named A to D), were detected. Four single nucleotide polymorphisms were found within the coding region and three of these were non-synonymous. The effect of this genetic variation on wool traits was investigated in 396 Merino × Southdown-cross sheep. Of the three variants found in these sheep (A, B and C), the presence of B was found to be associated with decreased wool yield, while C was associated with increased wool yield and decreased fibre diameter standard deviation. Sheep of genotype AC had a higher wool yield than those of genotype AA or AB.

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Corresponding author

Authors for correspondence: J. Wang, E-mail: wangjq@gsau.edu.cn J. G. Hickford, E-mail: Jonathan.Hickford@Lincoln.ac.nz

References

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The Journal of Agricultural Science
  • ISSN: 0021-8596
  • EISSN: 1469-5146
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