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Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum

Published online by Cambridge University Press:  27 March 2009

J. Balcells ,
J. A. Guada ,
C. Castrillo  and
J. Gasa
J. Balcells
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
J. A. Guada
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
C. Castrillo
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
J. Gasa
Affiliation:
Departamento de Production Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza 50013, Spain
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Summary

Two experiments were carried out to determine endogenous losses and the response of urinary purine derivatives to increased duodenal inputs of purine bases. Four ewes each fitted with a re-entrant cannula at the proximal duodenum, and conventionally fed, were subjected to full replacement of duodenal digesta followed by the administration of a solution either free of purines (Expt 1) or enriched with increasing amounts of purines, to supply 0·48–21·27 mmol/animal per day (Expt 2). Basal daily urinary excretions of allantoin, uric acid, hypoxanthine and xanthine were 11·5 ± 0·94, 9·9 ± 0·67, 6·9 ± 0·46 and 1·2 ±0·16 mg/kg W0·75. Allantoin was the only purine derivative which increased in response to incremental inputs of duodenal purines. The relationship between allantoin excretion and infused purines showed a urinary recovery of 0·8 for purines infused at > 220 μmol/kg W0·76. Lower rates of infusion did not alter allantoin excretion. The results show urinary allantoin to be a useful index to estimate duodenal input of purines when animals are fed close to or above their energy maintenance requirements.

Type
Animals
Information
The Journal of Agricultural Science , Volume 116 , Issue 2 , April 1991 , pp. 309 - 317
Copyright
Copyright © Cambridge University Press 1991

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