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Pollen-mediated transgene flow in maize grown in the Huang-huai-hai region in China

Published online by Cambridge University Press:  12 August 2010

K. ZHANG ,
Y. LI  and
L. LIAN
K. ZHANG*
Affiliation:
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, 27 Shanda South Road, Jinan 250100, P. R. China School of Life Science, Shandong University, 27 Shanda South Road, Jinan 250100, P. R. China
Y. LI
Affiliation:
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, 27 Shanda South Road, Jinan 250100, P. R. China School of Life Science, Shandong University, 27 Shanda South Road, Jinan 250100, P. R. China
L. LIAN
Affiliation:
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, 27 Shanda South Road, Jinan 250100, P. R. China School of Life Science, Shandong University, 27 Shanda South Road, Jinan 250100, P. R. China
*
*To whom all correspondence should be addressed. E-mail: zhangkw@sdu.edu.cn
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Summary

In order to study pollen-mediated gene flow in transgenic maize (Zea mays L.) in the Huang-huai-hai region of China, field trials were conducted in Jinan, Shandong Province in 2006 and 2007. The frequencies of gene flow from the donor plots, planted with transgenic maize as a pollen source, to the receptor plots, planted with non-transgenic maize, under different temporal or spatial separations were evaluated. The results showed that the frequency of pollen-mediated gene flow of the als gene from transgenic maize to non-transgenic maize decreased significantly with increasing distance. No gene flow was detected at 300 m. At a distance of 30 m, delaying the planting date of the transgenic maize by 1 week decreased the frequency of gene flow by 70%. A delay of 2 weeks decreased the gene flow frequency by more than 90%, while no gene flow was seen when the sowing date was delayed by 3 weeks. The results suggest that an appropriate isolation distance of 300 m or a temporal separation of 3 weeks could prevent gene flow from transgenic maize to non-transgenic maize in the Huang-huai-hai region.

Type
Crops and Soils
Information
The Journal of Agricultural Science , Volume 149 , Issue 2 , April 2011 , pp. 205 - 216
Copyright
Copyright © Cambridge University Press 2010

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