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Pathogenic fungal protein-induced resistance and its effects on vegetable diseases

Published online by Cambridge University Press:  31 March 2017

T.-C. LIN ,
C.-L. LIN ,
W.-C. CHUNG ,
K.-R. CHUNG  and
J.-W. HUANG
T.-C. LIN
Affiliation:
Department of Plant Pathology, College of Agriculture and Natural Resources, National Chung-Hsing University (NCHU), Taichung 40227, Taiwan Plant Pathology Division, Taiwan Agricultural Research Institute, Wufeng, Taichung 41362, Taiwan
C.-L. LIN
Affiliation:
Department of Plant Pathology, College of Agriculture and Natural Resources, National Chung-Hsing University (NCHU), Taichung 40227, Taiwan
W.-C. CHUNG
Affiliation:
Section of Biotechnology, Seed Improvement and Propagation Station, Hsinshe, Taichung 42642, Taiwan
K.-R. CHUNG
Affiliation:
Department of Plant Pathology, College of Agriculture and Natural Resources, National Chung-Hsing University (NCHU), Taichung 40227, Taiwan Biotechnology Center, NCHU, Taichung 40227, Taiwan NCHU-UCD Plant and Food Biotechnology Center, NCHU, Taichung 40227, Taiwan
J.-W. HUANG*
Affiliation:
Department of Plant Pathology, College of Agriculture and Natural Resources, National Chung-Hsing University (NCHU), Taichung 40227, Taiwan NCHU-UCD Plant and Food Biotechnology Center, NCHU, Taichung 40227, Taiwan
*
*To whom all correspondence should be addressed. Email: jwhuang@dragon.nchu.edu.tw
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Summary

Plant defence systems can be induced by biotic and abiotic stress. Experiments were undertaken to explore the feasibility of different fungal proteins for the reduction of vegetable diseases. Total proteins purified from three soil-borne and five foliar fungal pathogens had no fungistatic effects nor did they trigger hypersensitive reactions on test plants. The abilities to promote plant growth and to reduce disease severity varied among test proteins and plants. Depending on test proteins, experiments have demonstrated that exogenous application of fungal proteins could reduce Alternaria brassicicola-induced black spot severity on cabbage, Colletotrichum spp.-induced anthracnose on Chinese cabbage and cucumber, Rhizoctonia solani-induced damping-off on sweet pepper and Chinese cabbage, and powdery mildew on cucumber seedlings. An Alternariaprotein effector 1 (Ape1)-coding gene was cloned from two Alternaria spp. and expressed in Escherichia coli. The expressed Ape1 reduced anthracnose incidence on cucumber leaves, indicating that Ape1 was the primary activator in the crude protein extracts responsible for disease reduction. Application of Alternaria proteins onto Chinese cabbage seedlings caused an increase of phenylalanine ammonia lyase and peroxidase activities in treated seedlings, which may have played a role in host defence.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 7 , September 2017 , pp. 1069 - 1081
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Copyright
Copyright © Cambridge University Press 2017 

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