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Manganese-mediated resistance to aluminium and antibiotics in strains of Azospirillum brasilense and their interaction with rice genotypes in acid soil

Published online by Cambridge University Press:  27 March 2009

R. Rai
R. Rai
Affiliation:
Rajendra Agricultural University, Dholi Campus, Dholi, Muzaffarpur-843121, Bihar, India
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Summary

Manganese-tolerant strains Mnr-1 and Mnr-5 of Azospirillum brasilense (RAU 1075) were isolated through step-wise transfer to higher levels of manganese chloride. One of the strains (Mnr-5) showed more growth at low pH and greater nitrogenase activity in 10 mM MnCl2. 4H2O than parental and Mnr-1 strains. These tolerant strains also showed cross-resistance to antibiotics such as gentamycin and neomycin but not to streptomycin. Mn-adapted strains also tolerated elevated levels of aluminium (2·5–3·5 mg/kg) and the stability of the Mn-resistance required the presence of Mn2+ ions in the growth medium. It has been demonstrated that the effect of inoculation with Mn-adapted strains was variable with different genotypes of rice in acid soil and that initial low level of nitrogen was essential for maximum associative N2-fixation.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 106 , Issue 2 , April 1986 , pp. 279 - 285
Copyright
Copyright © Cambridge University Press 1986

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