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Genetic structure and diversity of a collection of Brassica rapa subsp. rapa L. revealed by simple sequence repeat markers

  • P. SOENGAS (a1), M. E. CARTEA (a1), M. FRANCISCO (a1), M. LEMA (a1) and P. VELASCO (a1)...

Summary

Brassica rapa subsp. rapa L. includes three different crops: turnips (roots), turnip greens (leaves) and turnip tops (inflorescences). A collection of B. rapa subsp. rapa from north-western Spain is currently kept at ‘Misión Biológica de Galicia’ (a research centre of the Consejo Superior de Investigaciones Científicas (CSIC), Spain). This collection has been characterized based on morphological and agronomical traits. A better understanding of the genetic diversity present in the collection is necessary in order to optimize its use and maintenance. The objectives of the present work were to assess the genetic diversity present in the B. rapa subsp. rapa collection, to establish genetic relationships among populations and to study the genetic structure of the collection. Eighty populations were analysed based on 18 simple sequence repeats (SSRs). Populations showed a broad range of genetic diversity, thus offering good potential for further genetic improvement. Most of the variability was found within the population level, probably due to high rates of allogamy, to migration and/or interchange of seed among local growers. Populations showed a low level of differentiation, grouping in just one cluster, and therefore they can be considered as samples of a highly variable metapopulation that can be used for B. rapa breeding programmes.

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Corresponding author

*To whom all correspondence should be addressed. Email: psoengas@mbg.cesga.es

References

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Evanno, G., Regnaut, S. & Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14, 26112620.
Excoffier, L., Smouse, P. E. & Quattro, J. M. (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479491.
Excoffier, L., Laval, G. & Schneider, S. (2005). Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1, 4750.
Fu, Y.-B. & Gugel, R. K. (2009). Genetic variability of Canadian elite cultivars of summer turnip rape (Brassica rapa L.) revealed by simple sequence repeat markers. Canadian Journal of Plant Science 89, 865874.
Gómez-Campo, C. & Prakash, S. (1999). Origin and domestication. In Biology of Brassica Coenospecies (Ed. Gómez-Campo, C.), pp. 3358. Amsterdam, The Netherlands: Elsevier.
Iniguez-Luy, F. L., Voort, A. V. & Osborn, T. C. (2008). Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype. Theoretical and Applied Genetics 117, 977985.
Liu, Y. G. & Whittier, R. F. (1994). Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads. Nucleic Acids Research 22, 21682169.
Lowe, A. J., Moule, C., Trick, M. & Edwards, K. J. (2004). Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species. Theoretical and Applied Genetics 108, 11031112.
Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Science of the United States of America 70, 33213323.
Nei, M. & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Science of the United States of America 76, 52695273.
Novakova, K., Hamouz, P., Holec, J. & Soukup, J. (2008). Genetic diversity of Beta-complex assessed by RAPD markers. Journal of Plant Diseases and Protection 21 (Special Issue), 437440.
Ofori, A., Becker, H. C. & Kopisch-Obuch, F. J. (2008). Effect of crop improvement on genetic diversity in oilseed Brassica rapa (turnip-rape) cultivars, detected by SSR markers. Journal of Applied Genetics 49, 207212.
Padilla, G., Cartea, M. E., Rodríquez, V. M. & Ordás, A. (2005). Genetic diversity in a germplasm collection of Brassica rapa subsp rapa L. from northwestern Spain. Euphytica 145, 171180.
Persson, K., Fält, A.-S. & Von Bothmer, R. (2001). Genetic diversity of allozymes in turnip (Brassica rapa L. var rapa) from the Nordic area. Hereditas 134, 4352.
Pritchard, J. K., Stephens, M. & Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics 155, 945959.
Reif, J. C., Melchinger, A. E., Xia, X. C., Warburton, M. L., Hoisington, D. A., Vasal, S. K., Srinivasan, G., Bohn, M. & Frisch, M. (2003). Genetic distance based on simple sequence repeats and heterosis in tropical maize populations. Crop Science 43, 12751282.
Rohlf, F. J. (1998). NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Version 2.1. New York: EXETER software.
Suwabe, K., Iketani, H., Nunome, T., Kage, T. & Hirai, M. (2002). Isolation and characterization of microsatellites in Brassica rapa L. Theoretical and Applied Genetics 104, 10921098.
Wright, S. (1969). Evolution and the Genetics of Populations, 2: The Theory of Gene Frequencies. Chicago, IL: University of Chicago Press.
Yap, I. V. & Nelson, R. J. (1996). Winboot: a Program for Performing Bootstrap Analysis of Binary Data to Determine the Confidence Limits of UPGMA-based Dendograms. Discussion Paper Series No. 14. Manila, The Philippines: IRRI.
Zhao, J., Wang, X., Deng, B., Lou, P., Wu, J., Sun, R., Xu, Z., Vromans, J., Koornneef, M. & Bonnema, G. (2005). Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theoretical and Applied Genetics 110, 13011314.

Genetic structure and diversity of a collection of Brassica rapa subsp. rapa L. revealed by simple sequence repeat markers

  • P. SOENGAS (a1), M. E. CARTEA (a1), M. FRANCISCO (a1), M. LEMA (a1) and P. VELASCO (a1)...

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